Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis

Detalhes bibliográficos
Autor(a) principal: Chies, Jocelei Maria
Data de Publicação: 2006
Outros Autores: Dias, Ana Christina de Oliveira, Maia, Hélio Mauro Moreira, Braga, Luis Paulo da Silva, Astolfi-Filho, Spartaco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/26782
https://dx.doi.org/10.1590/S1517-83822006000400027
Resumo: The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5'- AT<FONT FACE=Symbol>¯</FONT>CGAT - 3', was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37ºC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a value of 39 kDa.
id UNB_780fd3022320434040efe794542aec9e
oai_identifier_str oai:repositorio.unb.br:10482/26782
network_acronym_str UNB
network_name_str Repositório Institucional da UnB
repository_id_str
spelling Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformisIsolamento e caracterização de BliAI, um isoesquisômero de ClaI de Bacillus licheniformisEndonuclease de restrição do tipo IIisoesquisômeroBacillus licheniformisThe restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5'- AT<FONT FACE=Symbol>¯</FONT>CGAT - 3', was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37ºC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a value of 39 kDa.A endonuclease de restrição BliAI, um isoesquisômero de ClaI, que reconhece a seqüência 5'- AT<FONT FACE=Symbol>¯</FONT>CGAT - 3', foi purificada de um isolado natural identificado como Bacillus licheniformis. A endonuclease de restrição em questão foi isolada a partir de um extrato celular em um único passo cromatográfico utilizando uma coluna contendo a resina fosfocelulose. A endonuclease de restrição é ativa à 37ºC e em uma ampla escala de pH e concentração de sais. O peso molecular da enzima purificada corresponde a um valor de kDa 39.Em processamentoSociedade Brasileira de Microbiologia2017-12-07T04:45:51Z2017-12-07T04:45:51Z2006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBraz. J. Microbiol.,v.37,n.4,p.551-555,2006http://repositorio.unb.br/handle/10482/26782https://dx.doi.org/10.1590/S1517-83822006000400027Chies, Jocelei MariaDias, Ana Christina de OliveiraMaia, Hélio Mauro MoreiraBraga, Luis Paulo da SilvaAstolfi-Filho, Spartacoinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T16:26:09Zoai:repositorio.unb.br:10482/26782Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T16:26:09Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis
Isolamento e caracterização de BliAI, um isoesquisômero de ClaI de Bacillus licheniformis
title Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis
spellingShingle Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis
Chies, Jocelei Maria
Endonuclease de restrição do tipo II
isoesquisômero
Bacillus licheniformis
title_short Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis
title_full Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis
title_fullStr Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis
title_full_unstemmed Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis
title_sort Isolation and characterization of BliAI, an isoschizomer of ClaI from Bacillus licheniformis
author Chies, Jocelei Maria
author_facet Chies, Jocelei Maria
Dias, Ana Christina de Oliveira
Maia, Hélio Mauro Moreira
Braga, Luis Paulo da Silva
Astolfi-Filho, Spartaco
author_role author
author2 Dias, Ana Christina de Oliveira
Maia, Hélio Mauro Moreira
Braga, Luis Paulo da Silva
Astolfi-Filho, Spartaco
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Chies, Jocelei Maria
Dias, Ana Christina de Oliveira
Maia, Hélio Mauro Moreira
Braga, Luis Paulo da Silva
Astolfi-Filho, Spartaco
dc.subject.por.fl_str_mv Endonuclease de restrição do tipo II
isoesquisômero
Bacillus licheniformis
topic Endonuclease de restrição do tipo II
isoesquisômero
Bacillus licheniformis
description The restriction endonuclease BliAI, an isoschizomer of ClaI, which recognizes the sequence 5'- AT<FONT FACE=Symbol>¯</FONT>CGAT - 3', was purified from a natural isolate identified as Bacillus licheniformis. The restriction endonuclease was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. The restriction endonuclease is active at 37ºC and over a wide range of pH and salt concentration. The molecular weight of the purified restriction enzyme is consistent with a value of 39 kDa.
publishDate 2006
dc.date.none.fl_str_mv 2006
2017-12-07T04:45:51Z
2017-12-07T04:45:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Braz. J. Microbiol.,v.37,n.4,p.551-555,2006
http://repositorio.unb.br/handle/10482/26782
https://dx.doi.org/10.1590/S1517-83822006000400027
identifier_str_mv Braz. J. Microbiol.,v.37,n.4,p.551-555,2006
url http://repositorio.unb.br/handle/10482/26782
https://dx.doi.org/10.1590/S1517-83822006000400027
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
_version_ 1814508203358027776

Registros relacionados