BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'

Detalhes bibliográficos
Autor(a) principal: Chies, Jocelei M.
Data de Publicação: 2006
Outros Autores: Dias, Ana C. de O., Maia, Hélio M.M., Astolfi-Filho, Spartaco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/26781
https://dx.doi.org/10.1590/S1517-83822006000100018
Resumo: A strain of Bacillus pumilus was isolated and identified from water samples collected from a small affluent of the Amazon River. Type II restriction endonuclease activity was detected in these bacteria. The enzyme was purified and the molecular weight of the native protein estimated by gel filtration and SDS-PAGE. The optimum pH, temperature and salt requirements were determined. Quality control assays showed the complete absence of "nonspecific nucleases." Restriction cleavage analysis and DNA sequencing of restriction fragments allowed the unequivocal demonstration of 5´GAG<FONT FACE=Symbol>¯</FONT>CTC3´ as the recognition sequence. This enzyme was named BpuAmI and is apparently a neoschizomer of the prototype restriction endonuclease SacI. This is the first report of an isoschizomer and/or neoschizomer of the prototype SacI identified in the genus Bacillus.
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spelling BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'BpuAmI: um novo neoesquisomero de SacI de Bacillus pumilus descoberta em um isolado oriundo da Bacia Amazônica, reconhecendo 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'Endonuclease de Restrição Tipo IiNeoesquizomersBacillus pumilusA strain of Bacillus pumilus was isolated and identified from water samples collected from a small affluent of the Amazon River. Type II restriction endonuclease activity was detected in these bacteria. The enzyme was purified and the molecular weight of the native protein estimated by gel filtration and SDS-PAGE. The optimum pH, temperature and salt requirements were determined. Quality control assays showed the complete absence of "nonspecific nucleases." Restriction cleavage analysis and DNA sequencing of restriction fragments allowed the unequivocal demonstration of 5´GAG<FONT FACE=Symbol>¯</FONT>CTC3´ as the recognition sequence. This enzyme was named BpuAmI and is apparently a neoschizomer of the prototype restriction endonuclease SacI. This is the first report of an isoschizomer and/or neoschizomer of the prototype SacI identified in the genus Bacillus.Uma linhagem de Bacillus pumilus foi isolada e identificada de amostras de águas coletadas em um pequeno Igarapé do Rio Amazonas. Foi detectada atividade de restrição do tipo II nesta bactéria. A enzima foi purificada e o peso molecular da proteína nativa foi estimado por gel filtração e por eletroforese em gel de poliacrilamida. Foram determinados, o pH e temperatura ótimos e as necessidades de sais. Os ensaios do controle de qualidade mostraram uma ausência completa de "nucleases não específicas". As analises das clivagens e o seqüenciamento do DNA dos fragmentos de restrição permitiram uma demonstração inequívoca de que 5´GAG<FONT FACE=Symbol>¯</FONT>CTC 3´ é a seqüência de reconhecimento da enzima. Esta enzima foi denominada de BpuAmI e aparentemente é um neoesquisômero da enzima protótipo SacI. Este é o primeiro relato de um isoesquisômero e/ou neoesquisômero da enzima protótipo SacI identificada no gênero Bacillus.Em processamentoSociedade Brasileira de Microbiologia2017-12-07T04:45:51Z2017-12-07T04:45:51Z2006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBraz. J. Microbiol.,v.37,n.1,p.96-100,2006http://repositorio.unb.br/handle/10482/26781https://dx.doi.org/10.1590/S1517-83822006000100018Chies, Jocelei M.Dias, Ana C. de O.Maia, Hélio M.M.Astolfi-Filho, Spartacoinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-28T19:04:07Zoai:repositorio.unb.br:10482/26781Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-28T19:04:07Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'
BpuAmI: um novo neoesquisomero de SacI de Bacillus pumilus descoberta em um isolado oriundo da Bacia Amazônica, reconhecendo 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'
title BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'
spellingShingle BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'
Chies, Jocelei M.
Endonuclease de Restrição Tipo Ii
Neoesquizomers
Bacillus pumilus
title_short BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'
title_full BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'
title_fullStr BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'
title_full_unstemmed BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'
title_sort BpuAmI: a novel SacI neoschizomer from Bacillus pumilus discovered in an isolate from Amazon Basin, recognizing 5'-GAG<FONT FACE=Symbol>¯</FONT>CTC-3'
author Chies, Jocelei M.
author_facet Chies, Jocelei M.
Dias, Ana C. de O.
Maia, Hélio M.M.
Astolfi-Filho, Spartaco
author_role author
author2 Dias, Ana C. de O.
Maia, Hélio M.M.
Astolfi-Filho, Spartaco
author2_role author
author
author
dc.contributor.author.fl_str_mv Chies, Jocelei M.
Dias, Ana C. de O.
Maia, Hélio M.M.
Astolfi-Filho, Spartaco
dc.subject.por.fl_str_mv Endonuclease de Restrição Tipo Ii
Neoesquizomers
Bacillus pumilus
topic Endonuclease de Restrição Tipo Ii
Neoesquizomers
Bacillus pumilus
description A strain of Bacillus pumilus was isolated and identified from water samples collected from a small affluent of the Amazon River. Type II restriction endonuclease activity was detected in these bacteria. The enzyme was purified and the molecular weight of the native protein estimated by gel filtration and SDS-PAGE. The optimum pH, temperature and salt requirements were determined. Quality control assays showed the complete absence of "nonspecific nucleases." Restriction cleavage analysis and DNA sequencing of restriction fragments allowed the unequivocal demonstration of 5´GAG<FONT FACE=Symbol>¯</FONT>CTC3´ as the recognition sequence. This enzyme was named BpuAmI and is apparently a neoschizomer of the prototype restriction endonuclease SacI. This is the first report of an isoschizomer and/or neoschizomer of the prototype SacI identified in the genus Bacillus.
publishDate 2006
dc.date.none.fl_str_mv 2006
2017-12-07T04:45:51Z
2017-12-07T04:45:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Braz. J. Microbiol.,v.37,n.1,p.96-100,2006
http://repositorio.unb.br/handle/10482/26781
https://dx.doi.org/10.1590/S1517-83822006000100018
identifier_str_mv Braz. J. Microbiol.,v.37,n.1,p.96-100,2006
url http://repositorio.unb.br/handle/10482/26781
https://dx.doi.org/10.1590/S1517-83822006000100018
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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