Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting

Detalhes bibliográficos
Autor(a) principal: Trindade, Loiselene C.
Data de Publicação: 2005
Outros Autores: Lima, Mirtes F., Ferreira, Marisa A. S. V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/26357
https://dx.doi.org/10.1590/S0100-41582005000100008
Resumo: Bacterial canker of grapevine (Vitis vinifera), caused by Xanthomonas campestris pv. viticola was first detected in Brazil in 1998, affecting grapevines in the São Francisco river basin, state of Pernambuco. The disease was also reported in Juazeiro, Bahia and later in Piauí and Ceará. Due to its limited geographical distribution and relatively recent detection in Brazil, very little is known about the pathogen's biology and diversity. Repetitive DNA based-PCR (rep-PCR) profiles were generated from purified bacterial DNA of 40 field strains of X. campestris pv. viticola, collected between 1998 and 2001 in the states of Pernambuco, Bahia and Piauí. Combined analysis of the PCR patterns obtained with primers REP, ERIC and BOX, showed a high degree of similarity among Brazilian strains and the Indian type strain NCPPB 2475. Similar genomic patterns with several diagnostic bands, present in all strains, could be detected. Fingerprints were distinct from those of strains representing other pathovars and from a yellow non-pathogenic isolate from grape leaves. The polymorphism observed among the Brazilian strains allowed their separation into five subgroups, although with no correlation with cultivar of origin, geographic location or year collected.
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spelling Trindade, Loiselene C.Lima, Mirtes F.Ferreira, Marisa A. S. V.2017-12-07T04:42:09Z2017-12-07T04:42:09Z2005Fitopatol. bras.,v.30,n.1,p.46-54,2005http://repositorio.unb.br/handle/10482/26357https://dx.doi.org/10.1590/S0100-41582005000100008Bacterial canker of grapevine (Vitis vinifera), caused by Xanthomonas campestris pv. viticola was first detected in Brazil in 1998, affecting grapevines in the São Francisco river basin, state of Pernambuco. The disease was also reported in Juazeiro, Bahia and later in Piauí and Ceará. Due to its limited geographical distribution and relatively recent detection in Brazil, very little is known about the pathogen's biology and diversity. Repetitive DNA based-PCR (rep-PCR) profiles were generated from purified bacterial DNA of 40 field strains of X. campestris pv. viticola, collected between 1998 and 2001 in the states of Pernambuco, Bahia and Piauí. Combined analysis of the PCR patterns obtained with primers REP, ERIC and BOX, showed a high degree of similarity among Brazilian strains and the Indian type strain NCPPB 2475. Similar genomic patterns with several diagnostic bands, present in all strains, could be detected. Fingerprints were distinct from those of strains representing other pathovars and from a yellow non-pathogenic isolate from grape leaves. The polymorphism observed among the Brazilian strains allowed their separation into five subgroups, although with no correlation with cultivar of origin, geographic location or year collected.O cancro bacteriano da videira (Vitis vinifera), causado por Xanthomonas campestris pv. viticola, foi primeiramente detectado no Brasil em 1998, em parreirais no município de Petrolina, Vale do São Francisco, Pernambuco. A doença foi também observada em Juazeiro, Bahia, e mais tarde nos estados de Piauí e Ceará. Devido à sua limitada distribuição geográfica e relativamente recente detecção no Brasil, pouco se conhece sobre a biologia e diversidade do patógeno. Padrões genômicos de rep-PCR foram gerados a partir do DNA purificado de 40 isolados de X. campestris pv. viticola, coletados entre 1998 e 2001, em diferentes localidades dos estados de Pernambuco, Bahia e Piauí. A análise combinada dos padrões obtidos com os "primers" REP, ERIC e BOX, mostraram alta similaridade entre os isolados brasileiros e o isolado tipo NCPPB 2475, originário da Índia. Os isolados brasileiros apresentaram padrões similares e algumas bandas diagnósticas, presentes em todos os isolados. Os padrões foram distintos dos isolados de outros patovares e de um isolado não-patogênico obtido de folhas de videira. O polimorfismo observado entre os isolados brasileiros permitiu a diferenciação de cinco subgrupos, sem nenhuma relação com cultivar de origem, local ou época de coleta.Em processamentoSociedade Brasileira de FitopatologiaMolecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprintingCaracterização molecular de isolados brasileiros de Xanthomonas campestris pv. viticola através de rep-PCRinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlediversitygrapevine bacterial cankerVitis viniferarepetitive DNADiversidadeCancro bacterianoVitis viniferaDNA repetitivoinfo:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNBORIGINALa08v30n1.pdfapplication/pdf588404http://repositorio2.unb.br/jspui/bitstream/10482/26357/1/a08v30n1.pdffac781a4669ae650abfc8bc2115e154eMD51open access10482/263572024-01-28 15:06:35.307open accessoai:repositorio2.unb.br:10482/26357Biblioteca Digital de Teses e DissertaçõesPUBhttps://repositorio.unb.br/oai/requestopendoar:2024-01-28T18:06:35Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.pt_BR.fl_str_mv Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
Caracterização molecular de isolados brasileiros de Xanthomonas campestris pv. viticola através de rep-PCR
title Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
spellingShingle Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
Trindade, Loiselene C.
diversity
grapevine bacterial canker
Vitis vinifera
repetitive DNA
Diversidade
Cancro bacteriano
Vitis vinifera
DNA repetitivo
title_short Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
title_full Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
title_fullStr Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
title_full_unstemmed Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
title_sort Molecular characterization of Brazilian strains of Xanthomonas campestris pv. viticola by rep-PCR fingerprinting
author Trindade, Loiselene C.
author_facet Trindade, Loiselene C.
Lima, Mirtes F.
Ferreira, Marisa A. S. V.
author_role author
author2 Lima, Mirtes F.
Ferreira, Marisa A. S. V.
author2_role author
author
dc.contributor.author.fl_str_mv Trindade, Loiselene C.
Lima, Mirtes F.
Ferreira, Marisa A. S. V.
dc.subject.keyword.pt_BR.fl_str_mv diversity
grapevine bacterial canker
Vitis vinifera
repetitive DNA
Diversidade
Cancro bacteriano
Vitis vinifera
DNA repetitivo
topic diversity
grapevine bacterial canker
Vitis vinifera
repetitive DNA
Diversidade
Cancro bacteriano
Vitis vinifera
DNA repetitivo
description Bacterial canker of grapevine (Vitis vinifera), caused by Xanthomonas campestris pv. viticola was first detected in Brazil in 1998, affecting grapevines in the São Francisco river basin, state of Pernambuco. The disease was also reported in Juazeiro, Bahia and later in Piauí and Ceará. Due to its limited geographical distribution and relatively recent detection in Brazil, very little is known about the pathogen's biology and diversity. Repetitive DNA based-PCR (rep-PCR) profiles were generated from purified bacterial DNA of 40 field strains of X. campestris pv. viticola, collected between 1998 and 2001 in the states of Pernambuco, Bahia and Piauí. Combined analysis of the PCR patterns obtained with primers REP, ERIC and BOX, showed a high degree of similarity among Brazilian strains and the Indian type strain NCPPB 2475. Similar genomic patterns with several diagnostic bands, present in all strains, could be detected. Fingerprints were distinct from those of strains representing other pathovars and from a yellow non-pathogenic isolate from grape leaves. The polymorphism observed among the Brazilian strains allowed their separation into five subgroups, although with no correlation with cultivar of origin, geographic location or year collected.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2017-12-07T04:42:09Z
dc.date.available.fl_str_mv 2017-12-07T04:42:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv Fitopatol. bras.,v.30,n.1,p.46-54,2005
dc.identifier.uri.fl_str_mv http://repositorio.unb.br/handle/10482/26357
dc.identifier.doi.pt_BR.fl_str_mv https://dx.doi.org/10.1590/S0100-41582005000100008
identifier_str_mv Fitopatol. bras.,v.30,n.1,p.46-54,2005
url http://repositorio.unb.br/handle/10482/26357
https://dx.doi.org/10.1590/S0100-41582005000100008
dc.language.iso.fl_str_mv eng
language eng
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eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
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