Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture

Detalhes bibliográficos
Autor(a) principal: Diniz, S. F.
Data de Publicação: 2008
Outros Autores: Amorim, F. P. L. G., Cavalcante-Neto, F. F., Bocca, A. L., Batista, A. C., Simm, G. E. P. M., Silva, T. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/27213
https://dx.doi.org/10.1590/S0100-879X2008005000014
Resumo: A closed fracture was performed on the left tibia of 3-month-old Wistar rats weighing 250 to 350 g that were either healthy (N = 24) or made diabetic with alloxan (N = 24) to investigate the effect of alloxan-induced diabetes on the course of bone fracture healing. Histomorphometric analysis of the fracture site was performed at 7, 14, 25, and 35 days. After 7 days, diabetic rats had significantly less cartilage (P = 0.045) and greater fibrous connective (P = 0.006) tissue formation at the fracture site compared to controls. In contrast, marked callus formation was seen in diabetic rats with significant osteogenesis (P = 0.011, P = 0.010, P = 0.010, respectively, for 14, 25, and 35 days) and chondrogenesis (P = 0.028, P = 0.033, P = 0.019) compared to controls. Radiographic analysis revealed a displaced fracture with poor bone fragment alignment and delayed consolidation at these times in the diabetic group. The levels of alkaline phosphatase were significantly higher in diabetic rats at 25 days (P = 0.009). These results suggest that the initial excessive formation of fibrous connective tissue associated with delay in chondrogenesis and osteogenesis may not provide suitable stability of the fractured site, contributing to the inappropriate alignment of fragments and an increase in the volume of callus in later stages of repair. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation.
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spelling Alloxan-induced diabetes delays repair in a rat model of closed tibial fractureAlloxanDiabetesA closed fracture was performed on the left tibia of 3-month-old Wistar rats weighing 250 to 350 g that were either healthy (N = 24) or made diabetic with alloxan (N = 24) to investigate the effect of alloxan-induced diabetes on the course of bone fracture healing. Histomorphometric analysis of the fracture site was performed at 7, 14, 25, and 35 days. After 7 days, diabetic rats had significantly less cartilage (P = 0.045) and greater fibrous connective (P = 0.006) tissue formation at the fracture site compared to controls. In contrast, marked callus formation was seen in diabetic rats with significant osteogenesis (P = 0.011, P = 0.010, P = 0.010, respectively, for 14, 25, and 35 days) and chondrogenesis (P = 0.028, P = 0.033, P = 0.019) compared to controls. Radiographic analysis revealed a displaced fracture with poor bone fragment alignment and delayed consolidation at these times in the diabetic group. The levels of alkaline phosphatase were significantly higher in diabetic rats at 25 days (P = 0.009). These results suggest that the initial excessive formation of fibrous connective tissue associated with delay in chondrogenesis and osteogenesis may not provide suitable stability of the fractured site, contributing to the inappropriate alignment of fragments and an increase in the volume of callus in later stages of repair. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation.Em processamentoAssociação Brasileira de Divulgação Científica2017-12-07T04:49:29Z2017-12-07T04:49:29Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBraz J Med Biol Res,v.41,n.5,p.373-379,2008http://repositorio.unb.br/handle/10482/27213https://dx.doi.org/10.1590/S0100-879X2008005000014Diniz, S. F.Amorim, F. P. L. G.Cavalcante-Neto, F. F.Bocca, A. L.Batista, A. C.Simm, G. E. P. M.Silva, T. A.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-27T21:07:39Zoai:repositorio.unb.br:10482/27213Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-27T21:07:39Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
title Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
spellingShingle Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
Diniz, S. F.
Alloxan
Diabetes
title_short Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
title_full Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
title_fullStr Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
title_full_unstemmed Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
title_sort Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
author Diniz, S. F.
author_facet Diniz, S. F.
Amorim, F. P. L. G.
Cavalcante-Neto, F. F.
Bocca, A. L.
Batista, A. C.
Simm, G. E. P. M.
Silva, T. A.
author_role author
author2 Amorim, F. P. L. G.
Cavalcante-Neto, F. F.
Bocca, A. L.
Batista, A. C.
Simm, G. E. P. M.
Silva, T. A.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Diniz, S. F.
Amorim, F. P. L. G.
Cavalcante-Neto, F. F.
Bocca, A. L.
Batista, A. C.
Simm, G. E. P. M.
Silva, T. A.
dc.subject.por.fl_str_mv Alloxan
Diabetes
topic Alloxan
Diabetes
description A closed fracture was performed on the left tibia of 3-month-old Wistar rats weighing 250 to 350 g that were either healthy (N = 24) or made diabetic with alloxan (N = 24) to investigate the effect of alloxan-induced diabetes on the course of bone fracture healing. Histomorphometric analysis of the fracture site was performed at 7, 14, 25, and 35 days. After 7 days, diabetic rats had significantly less cartilage (P = 0.045) and greater fibrous connective (P = 0.006) tissue formation at the fracture site compared to controls. In contrast, marked callus formation was seen in diabetic rats with significant osteogenesis (P = 0.011, P = 0.010, P = 0.010, respectively, for 14, 25, and 35 days) and chondrogenesis (P = 0.028, P = 0.033, P = 0.019) compared to controls. Radiographic analysis revealed a displaced fracture with poor bone fragment alignment and delayed consolidation at these times in the diabetic group. The levels of alkaline phosphatase were significantly higher in diabetic rats at 25 days (P = 0.009). These results suggest that the initial excessive formation of fibrous connective tissue associated with delay in chondrogenesis and osteogenesis may not provide suitable stability of the fractured site, contributing to the inappropriate alignment of fragments and an increase in the volume of callus in later stages of repair. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation.
publishDate 2008
dc.date.none.fl_str_mv 2008
2017-12-07T04:49:29Z
2017-12-07T04:49:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Braz J Med Biol Res,v.41,n.5,p.373-379,2008
http://repositorio.unb.br/handle/10482/27213
https://dx.doi.org/10.1590/S0100-879X2008005000014
identifier_str_mv Braz J Med Biol Res,v.41,n.5,p.373-379,2008
url http://repositorio.unb.br/handle/10482/27213
https://dx.doi.org/10.1590/S0100-879X2008005000014
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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