Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio.unb.br/handle/10482/27213 https://dx.doi.org/10.1590/S0100-879X2008005000014 |
Resumo: | A closed fracture was performed on the left tibia of 3-month-old Wistar rats weighing 250 to 350 g that were either healthy (N = 24) or made diabetic with alloxan (N = 24) to investigate the effect of alloxan-induced diabetes on the course of bone fracture healing. Histomorphometric analysis of the fracture site was performed at 7, 14, 25, and 35 days. After 7 days, diabetic rats had significantly less cartilage (P = 0.045) and greater fibrous connective (P = 0.006) tissue formation at the fracture site compared to controls. In contrast, marked callus formation was seen in diabetic rats with significant osteogenesis (P = 0.011, P = 0.010, P = 0.010, respectively, for 14, 25, and 35 days) and chondrogenesis (P = 0.028, P = 0.033, P = 0.019) compared to controls. Radiographic analysis revealed a displaced fracture with poor bone fragment alignment and delayed consolidation at these times in the diabetic group. The levels of alkaline phosphatase were significantly higher in diabetic rats at 25 days (P = 0.009). These results suggest that the initial excessive formation of fibrous connective tissue associated with delay in chondrogenesis and osteogenesis may not provide suitable stability of the fractured site, contributing to the inappropriate alignment of fragments and an increase in the volume of callus in later stages of repair. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation. |
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Alloxan-induced diabetes delays repair in a rat model of closed tibial fractureAlloxanDiabetesA closed fracture was performed on the left tibia of 3-month-old Wistar rats weighing 250 to 350 g that were either healthy (N = 24) or made diabetic with alloxan (N = 24) to investigate the effect of alloxan-induced diabetes on the course of bone fracture healing. Histomorphometric analysis of the fracture site was performed at 7, 14, 25, and 35 days. After 7 days, diabetic rats had significantly less cartilage (P = 0.045) and greater fibrous connective (P = 0.006) tissue formation at the fracture site compared to controls. In contrast, marked callus formation was seen in diabetic rats with significant osteogenesis (P = 0.011, P = 0.010, P = 0.010, respectively, for 14, 25, and 35 days) and chondrogenesis (P = 0.028, P = 0.033, P = 0.019) compared to controls. Radiographic analysis revealed a displaced fracture with poor bone fragment alignment and delayed consolidation at these times in the diabetic group. The levels of alkaline phosphatase were significantly higher in diabetic rats at 25 days (P = 0.009). These results suggest that the initial excessive formation of fibrous connective tissue associated with delay in chondrogenesis and osteogenesis may not provide suitable stability of the fractured site, contributing to the inappropriate alignment of fragments and an increase in the volume of callus in later stages of repair. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation.Em processamentoAssociação Brasileira de Divulgação Científica2017-12-07T04:49:29Z2017-12-07T04:49:29Z2008info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBraz J Med Biol Res,v.41,n.5,p.373-379,2008http://repositorio.unb.br/handle/10482/27213https://dx.doi.org/10.1590/S0100-879X2008005000014Diniz, S. F.Amorim, F. P. L. G.Cavalcante-Neto, F. F.Bocca, A. L.Batista, A. C.Simm, G. E. P. M.Silva, T. A.info:eu-repo/semantics/openAccessengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-08-27T21:07:39Zoai:repositorio.unb.br:10482/27213Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-08-27T21:07:39Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.none.fl_str_mv |
Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture |
title |
Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture |
spellingShingle |
Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture Diniz, S. F. Alloxan Diabetes |
title_short |
Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture |
title_full |
Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture |
title_fullStr |
Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture |
title_full_unstemmed |
Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture |
title_sort |
Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture |
author |
Diniz, S. F. |
author_facet |
Diniz, S. F. Amorim, F. P. L. G. Cavalcante-Neto, F. F. Bocca, A. L. Batista, A. C. Simm, G. E. P. M. Silva, T. A. |
author_role |
author |
author2 |
Amorim, F. P. L. G. Cavalcante-Neto, F. F. Bocca, A. L. Batista, A. C. Simm, G. E. P. M. Silva, T. A. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Diniz, S. F. Amorim, F. P. L. G. Cavalcante-Neto, F. F. Bocca, A. L. Batista, A. C. Simm, G. E. P. M. Silva, T. A. |
dc.subject.por.fl_str_mv |
Alloxan Diabetes |
topic |
Alloxan Diabetes |
description |
A closed fracture was performed on the left tibia of 3-month-old Wistar rats weighing 250 to 350 g that were either healthy (N = 24) or made diabetic with alloxan (N = 24) to investigate the effect of alloxan-induced diabetes on the course of bone fracture healing. Histomorphometric analysis of the fracture site was performed at 7, 14, 25, and 35 days. After 7 days, diabetic rats had significantly less cartilage (P = 0.045) and greater fibrous connective (P = 0.006) tissue formation at the fracture site compared to controls. In contrast, marked callus formation was seen in diabetic rats with significant osteogenesis (P = 0.011, P = 0.010, P = 0.010, respectively, for 14, 25, and 35 days) and chondrogenesis (P = 0.028, P = 0.033, P = 0.019) compared to controls. Radiographic analysis revealed a displaced fracture with poor bone fragment alignment and delayed consolidation at these times in the diabetic group. The levels of alkaline phosphatase were significantly higher in diabetic rats at 25 days (P = 0.009). These results suggest that the initial excessive formation of fibrous connective tissue associated with delay in chondrogenesis and osteogenesis may not provide suitable stability of the fractured site, contributing to the inappropriate alignment of fragments and an increase in the volume of callus in later stages of repair. The resulting displaced fracture in diabetic rats requires long periods for remodeling and complete bone consolidation. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2017-12-07T04:49:29Z 2017-12-07T04:49:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Braz J Med Biol Res,v.41,n.5,p.373-379,2008 http://repositorio.unb.br/handle/10482/27213 https://dx.doi.org/10.1590/S0100-879X2008005000014 |
identifier_str_mv |
Braz J Med Biol Res,v.41,n.5,p.373-379,2008 |
url |
http://repositorio.unb.br/handle/10482/27213 https://dx.doi.org/10.1590/S0100-879X2008005000014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
instname_str |
Universidade de Brasília (UnB) |
instacron_str |
UNB |
institution |
UNB |
reponame_str |
Repositório Institucional da UnB |
collection |
Repositório Institucional da UnB |
repository.name.fl_str_mv |
Repositório Institucional da UnB - Universidade de Brasília (UnB) |
repository.mail.fl_str_mv |
repositorio@unb.br |
_version_ |
1814508226483322880 |