Hepatitis C virus genotypes in blood donors from the Federal District, Central Brazil

• Autor(a) principal: Amorim, Regina MS
• Data de Publicação: 2004
• Outros Autores: Oliveira, Claudiner P, Wyant, Patrícia S, Cerqueira, Daniela M, Câmara, Geni NL, Flores, Luciano S, Martins, Regina MB, Martins, Cláudia RF
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UnB
• Texto Completo: http://repositorio.unb.br/handle/10482/26149; https://dx.doi.org/10.1590/S0074-02762004000800019
• topic: Hepatite C; Genótipos

The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.