Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss

Detalhes bibliográficos
Autor(a) principal: Malard, Patrícia Furtado
Data de Publicação: 2020
Outros Autores: Peixer, Mauricio Antônio da Silva, Grázia, João Gabriel Viana de, Brunel, Hilana dos Santos Sena, Féres, Luiz Fernando Rodrigues, Villarroel, Carla Lujan Pereira, Siqueira, Luiz Gustavo Bruno, Dode, Margot Alves Nunes, Pogue, Robert, Viana, João Henrique Moreira, Carvalho, Juliana Lott de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio2.unb.br/jspui/handle/10482/48004
https://doi.org/10.1038/s41598-020-64810-x
https://orcid.org/0000-0002-3073-4748
https://orcid.org/0000-0002-9118-7768
https://orcid.org/0000-0002-9976-681X
https://orcid.org/0000-0002-9425-1593
https://orcid.org/0000-0001-7566-2859
https://orcid.org/0000-0002-5125-9798
https://orcid.org/0000-0002-2800-5829
https://orcid.org/0000-0002-1096-0457
https://orcid.org/0000-0002-8789-3512
https://orcid.org/0000-0002-3742-2368
https://orcid.org/0000-0002-1423-0523
Resumo: Valuable female cattle are continuously subject to follicular puncture (ovum pick-up - OPU). This technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative efects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5×106 MSCs immediately after the 4th OPU procedure (n=5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P<0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC+FSH in both ovaries after the frst OPU. In the two following OPU sessions, the MSC and MSC +FSH - treated groups failed to present any signifcant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have benefcial efects on the fertility of OPU lesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions.
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spelling Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility lossReprodução animalCélulas-tronco mesenquimaisBovino - embriãoValuable female cattle are continuously subject to follicular puncture (ovum pick-up - OPU). This technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative efects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5×106 MSCs immediately after the 4th OPU procedure (n=5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P<0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC+FSH in both ovaries after the frst OPU. In the two following OPU sessions, the MSC and MSC +FSH - treated groups failed to present any signifcant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have benefcial efects on the fertility of OPU lesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions.Faculdade de Medicina (FM)Springer NatureUniversidade Católica de BrasíliaBio Biotecnologia da Reprodução AnimalUniversidade Católica de BrasíliaBio Biotecnologia da Reprodução AnimalCenatte EmbrioesBio Biotecnologia da Reprodução AnimalUniversidade de AlfenasBio Biotecnologia da Reprodução AnimalEmpresa Brasileira de Pesquisa Agropecuária - EMBRAPA Gado de LeiteEmpresa Brasileira de Pesquisa Agropecuária - EMBRAPA Recursos Genéticos e BiotecnologiaUniversidade Católica de BrasíliaEmpresa Brasileira de Pesquisa Agropecuária - EMBRAPA Recursos Genéticos e BiotecnologiaUniversidade Católica de BrasíliaUniversidade de BrasíliaMalard, Patrícia FurtadoPeixer, Mauricio Antônio da SilvaGrázia, João Gabriel Viana deBrunel, Hilana dos Santos SenaFéres, Luiz Fernando RodriguesVillarroel, Carla Lujan PereiraSiqueira, Luiz Gustavo BrunoDode, Margot Alves NunesPogue, RobertViana, João Henrique MoreiraCarvalho, Juliana Lott de2024-03-19T13:21:58Z2024-03-19T13:21:58Z2020-05-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfMALARD, Patrícia F. et al. Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. Scientific Reports, v. 10, art. n. 8018, 2020. DOI: https://doi.org/10.1038/s41598-020-64810-x. Disponível em: https://www.nature.com/articles/s41598-020-64810-x. Acesso em: 19 mar. 2024.http://repositorio2.unb.br/jspui/handle/10482/48004https://doi.org/10.1038/s41598-020-64810-xhttps://orcid.org/0000-0002-3073-4748https://orcid.org/0000-0002-9118-7768https://orcid.org/0000-0002-9976-681Xhttps://orcid.org/0000-0002-9425-1593https://orcid.org/0000-0001-7566-2859https://orcid.org/0000-0002-5125-9798https://orcid.org/0000-0002-2800-5829https://orcid.org/0000-0002-1096-0457https://orcid.org/0000-0002-8789-3512https://orcid.org/0000-0002-3742-2368https://orcid.org/0000-0002-1423-0523eng(CC BY) Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre ative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2024-03-19T13:21:58Zoai:repositorio.unb.br:10482/48004Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2024-03-19T13:21:58Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss
title Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss
spellingShingle Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss
Malard, Patrícia Furtado
Reprodução animal
Células-tronco mesenquimais
Bovino - embrião
title_short Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss
title_full Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss
title_fullStr Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss
title_full_unstemmed Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss
title_sort Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss
author Malard, Patrícia Furtado
author_facet Malard, Patrícia Furtado
Peixer, Mauricio Antônio da Silva
Grázia, João Gabriel Viana de
Brunel, Hilana dos Santos Sena
Féres, Luiz Fernando Rodrigues
Villarroel, Carla Lujan Pereira
Siqueira, Luiz Gustavo Bruno
Dode, Margot Alves Nunes
Pogue, Robert
Viana, João Henrique Moreira
Carvalho, Juliana Lott de
author_role author
author2 Peixer, Mauricio Antônio da Silva
Grázia, João Gabriel Viana de
Brunel, Hilana dos Santos Sena
Féres, Luiz Fernando Rodrigues
Villarroel, Carla Lujan Pereira
Siqueira, Luiz Gustavo Bruno
Dode, Margot Alves Nunes
Pogue, Robert
Viana, João Henrique Moreira
Carvalho, Juliana Lott de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Católica de Brasília
Bio Biotecnologia da Reprodução Animal
Universidade Católica de Brasília
Bio Biotecnologia da Reprodução Animal
Cenatte Embrioes
Bio Biotecnologia da Reprodução Animal
Universidade de Alfenas
Bio Biotecnologia da Reprodução Animal
Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA Gado de Leite
Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA Recursos Genéticos e Biotecnologia
Universidade Católica de Brasília
Empresa Brasileira de Pesquisa Agropecuária - EMBRAPA Recursos Genéticos e Biotecnologia
Universidade Católica de Brasília
Universidade de Brasília
dc.contributor.author.fl_str_mv Malard, Patrícia Furtado
Peixer, Mauricio Antônio da Silva
Grázia, João Gabriel Viana de
Brunel, Hilana dos Santos Sena
Féres, Luiz Fernando Rodrigues
Villarroel, Carla Lujan Pereira
Siqueira, Luiz Gustavo Bruno
Dode, Margot Alves Nunes
Pogue, Robert
Viana, João Henrique Moreira
Carvalho, Juliana Lott de
dc.subject.por.fl_str_mv Reprodução animal
Células-tronco mesenquimais
Bovino - embrião
topic Reprodução animal
Células-tronco mesenquimais
Bovino - embrião
description Valuable female cattle are continuously subject to follicular puncture (ovum pick-up - OPU). This technique is commonly used for in-vitro embryo production, but may result in ovarian lesion. Mesenchymal stem cells (MSC) ameliorate the function of injured tissues, but their use to treat ovarian lesions in cattle has not been established. We investigated whether a local injection of MSC would reduce the negative efects of repeated OPU under acute and chronic scenarios in bovines. First, we performed four OPU sessions and injected 2.5×106 MSCs immediately after the 4th OPU procedure (n=5). The treated organs (right ovary) were compared to their saline-treated counterparts (left), and presented superior production of oocytes and embryos in the three following OPU sessions (P<0.05). Then, cows with progressive fertility loss went through three OPU sessions. Animals received MSC, saline, or MSC+FSH in both ovaries after the frst OPU. In the two following OPU sessions, the MSC and MSC +FSH - treated groups failed to present any signifcant alteration in the number of oocytes and embryos compared to saline-treated animals. Thus, MSC have benefcial efects on the fertility of OPU lesioned cows, but not in cows with cystic ovarian disease and chronic ovarian lesions.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-15
2024-03-19T13:21:58Z
2024-03-19T13:21:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv MALARD, Patrícia F. et al. Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. Scientific Reports, v. 10, art. n. 8018, 2020. DOI: https://doi.org/10.1038/s41598-020-64810-x. Disponível em: https://www.nature.com/articles/s41598-020-64810-x. Acesso em: 19 mar. 2024.
http://repositorio2.unb.br/jspui/handle/10482/48004
https://doi.org/10.1038/s41598-020-64810-x
https://orcid.org/0000-0002-3073-4748
https://orcid.org/0000-0002-9118-7768
https://orcid.org/0000-0002-9976-681X
https://orcid.org/0000-0002-9425-1593
https://orcid.org/0000-0001-7566-2859
https://orcid.org/0000-0002-5125-9798
https://orcid.org/0000-0002-2800-5829
https://orcid.org/0000-0002-1096-0457
https://orcid.org/0000-0002-8789-3512
https://orcid.org/0000-0002-3742-2368
https://orcid.org/0000-0002-1423-0523
identifier_str_mv MALARD, Patrícia F. et al. Intraovarian injection of mesenchymal stem cells improves oocyte yield and in vitro embryo production in a bovine model of fertility loss. Scientific Reports, v. 10, art. n. 8018, 2020. DOI: https://doi.org/10.1038/s41598-020-64810-x. Disponível em: https://www.nature.com/articles/s41598-020-64810-x. Acesso em: 19 mar. 2024.
url http://repositorio2.unb.br/jspui/handle/10482/48004
https://doi.org/10.1038/s41598-020-64810-x
https://orcid.org/0000-0002-3073-4748
https://orcid.org/0000-0002-9118-7768
https://orcid.org/0000-0002-9976-681X
https://orcid.org/0000-0002-9425-1593
https://orcid.org/0000-0001-7566-2859
https://orcid.org/0000-0002-5125-9798
https://orcid.org/0000-0002-2800-5829
https://orcid.org/0000-0002-1096-0457
https://orcid.org/0000-0002-8789-3512
https://orcid.org/0000-0002-3742-2368
https://orcid.org/0000-0002-1423-0523
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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