Paternity test in "Mangalarga-Marchador" equines by DNA-fingerprinting

• Autor(a) principal: ANUNCIAÇÃO, CARLOS EDUARDO
• Data de Publicação: 2000
• Outros Autores: ASTOLFI-FILHO, SPARTACO
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UnB
• Texto Completo: http://repositorio.unb.br/handle/10482/25598; https://dx.doi.org/10.1590/S0100-204X2000001000012
• topic: Métodos de melhoramento; Clonagem molecular; Teste de progênie; Cavalos; Identificação; Polimorfismo; Genética

GC-rich molecular minisatellite probes isolated from the human genome have presented a poor ability for individualization in horses. In this study new DNA sequences were isolated which could be used in paternity tests in horses. Genomic DNA from "Mangalarga-Marchador" horses was treated with restriction enzymes that preferentially digest non-repetitive sequences, so preserving the structure where mini and microsatellites are located. Four clones (S01, S05, S07 and S09) selected from a genomic library screened with a (TG)n oligonucleotide showed similar hybridization profiles generating bands of DNA-fingerprinting type. Using these probes the individualization power obtained was 10-8, which is 10(5)fold higher than that obtained with M13, another GC-rich type probe. All clones were efficient in parentage detection in crossbreedings and presented a 27 bp consensus sequence, GTTTCATTTATTATTCTTTGGAAGAAA, which was repeated 12, 18, 11 and 21 times in clones S01, S05, S07 and S09, respectively.