Limb immobilization alters functional electrophysiological parameters of sciatic nerve
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio.unb.br/handle/10482/28739 https://dx.doi.org/10.1590/1414-431X20132626 |
Resumo: | Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), animals were killed and the right sciatic nerve was dissected and mounted in a moist chamber. Nerves were stimulated at a baseline frequency of 0.2 Hz and tested for 2 min at 20, 50, and 100 Hz. Immobilization altered nerve excitability. Rheobase and chronaxy changed from 3.13±0.05 V and 52.31±1.95 µs (control group, n=13) to 2.84±0.06 V and 59.71±2.79 µs (immobilized group, n=15), respectively. Immobilization altered the amplitude of CAP waves and decreased the conduction velocity of the first CAP wave (from 93.63±7.49 to 79.14±5.59 m/s) but not of the second wave. Transmission electron microscopy showed fragmentation of the myelin sheath of the sciatic nerve of immobilized limbs and degeneration of the axon. In conclusion, we demonstrated that long-lasting leg immobilization can induce alterations in nerve function. |
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Limb immobilization alters functional electrophysiological parameters of sciatic nerveRatosNervo ciáticoEstimulação elétricaReobaseCronaxiaImmobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), animals were killed and the right sciatic nerve was dissected and mounted in a moist chamber. Nerves were stimulated at a baseline frequency of 0.2 Hz and tested for 2 min at 20, 50, and 100 Hz. Immobilization altered nerve excitability. Rheobase and chronaxy changed from 3.13±0.05 V and 52.31±1.95 µs (control group, n=13) to 2.84±0.06 V and 59.71±2.79 µs (immobilized group, n=15), respectively. Immobilization altered the amplitude of CAP waves and decreased the conduction velocity of the first CAP wave (from 93.63±7.49 to 79.14±5.59 m/s) but not of the second wave. Transmission electron microscopy showed fragmentation of the myelin sheath of the sciatic nerve of immobilized limbs and degeneration of the axon. In conclusion, we demonstrated that long-lasting leg immobilization can induce alterations in nerve function.Associação Brasileira de Divulgação Científica2017-12-07T05:02:34Z2017-12-07T05:02:34Z2013-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfALVES, J. S. M. et al. Limb immobilization alters functional electrophysiological parameters of sciatic nerve. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 46, n. 8, p. 715-721, ago. 2013. DOI: https://doi.org/10.1590/1414-431X20132626. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800715&lng=en&nrm=iso. Acesso em: 04 dez. 2020.http://repositorio.unb.br/handle/10482/28739https://dx.doi.org/10.1590/1414-431X20132626Brazilian Journal of Medical and Biological Research - All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License (CC BY 4.0). Fonte: https://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800715&lng=en&tlng=en. Acesso em: 04 dez. 2020.info:eu-repo/semantics/openAccessAlves, J. S. M.Leal-Cardoso, J. H.Santos-Júnior, F. F. U.Carlos, P. S.Silva, Renata CarvalhoLucci, Carolina MadeiraBáo, Sônia NairCeccatto, V. M.Barbosa, R.engreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-05-24T23:34:37Zoai:repositorio.unb.br:10482/28739Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:34:37Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.none.fl_str_mv |
Limb immobilization alters functional electrophysiological parameters of sciatic nerve |
title |
Limb immobilization alters functional electrophysiological parameters of sciatic nerve |
spellingShingle |
Limb immobilization alters functional electrophysiological parameters of sciatic nerve Alves, J. S. M. Ratos Nervo ciático Estimulação elétrica Reobase Cronaxia |
title_short |
Limb immobilization alters functional electrophysiological parameters of sciatic nerve |
title_full |
Limb immobilization alters functional electrophysiological parameters of sciatic nerve |
title_fullStr |
Limb immobilization alters functional electrophysiological parameters of sciatic nerve |
title_full_unstemmed |
Limb immobilization alters functional electrophysiological parameters of sciatic nerve |
title_sort |
Limb immobilization alters functional electrophysiological parameters of sciatic nerve |
author |
Alves, J. S. M. |
author_facet |
Alves, J. S. M. Leal-Cardoso, J. H. Santos-Júnior, F. F. U. Carlos, P. S. Silva, Renata Carvalho Lucci, Carolina Madeira Báo, Sônia Nair Ceccatto, V. M. Barbosa, R. |
author_role |
author |
author2 |
Leal-Cardoso, J. H. Santos-Júnior, F. F. U. Carlos, P. S. Silva, Renata Carvalho Lucci, Carolina Madeira Báo, Sônia Nair Ceccatto, V. M. Barbosa, R. |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Alves, J. S. M. Leal-Cardoso, J. H. Santos-Júnior, F. F. U. Carlos, P. S. Silva, Renata Carvalho Lucci, Carolina Madeira Báo, Sônia Nair Ceccatto, V. M. Barbosa, R. |
dc.subject.por.fl_str_mv |
Ratos Nervo ciático Estimulação elétrica Reobase Cronaxia |
topic |
Ratos Nervo ciático Estimulação elétrica Reobase Cronaxia |
description |
Immobilization, used in clinical practice to treat traumatologic problems, causes changes in muscle, but it is not known whether changes also occur in nerves. We investigated the effects of immobilization on excitability and compound action potential (CAP) and the ultrastructure of the rat sciatic nerve. Fourteen days after immobilization of the right leg of adult male Wistar rats (n=34), animals were killed and the right sciatic nerve was dissected and mounted in a moist chamber. Nerves were stimulated at a baseline frequency of 0.2 Hz and tested for 2 min at 20, 50, and 100 Hz. Immobilization altered nerve excitability. Rheobase and chronaxy changed from 3.13±0.05 V and 52.31±1.95 µs (control group, n=13) to 2.84±0.06 V and 59.71±2.79 µs (immobilized group, n=15), respectively. Immobilization altered the amplitude of CAP waves and decreased the conduction velocity of the first CAP wave (from 93.63±7.49 to 79.14±5.59 m/s) but not of the second wave. Transmission electron microscopy showed fragmentation of the myelin sheath of the sciatic nerve of immobilized limbs and degeneration of the axon. In conclusion, we demonstrated that long-lasting leg immobilization can induce alterations in nerve function. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08 2017-12-07T05:02:34Z 2017-12-07T05:02:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ALVES, J. S. M. et al. Limb immobilization alters functional electrophysiological parameters of sciatic nerve. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 46, n. 8, p. 715-721, ago. 2013. DOI: https://doi.org/10.1590/1414-431X20132626. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800715&lng=en&nrm=iso. Acesso em: 04 dez. 2020. http://repositorio.unb.br/handle/10482/28739 https://dx.doi.org/10.1590/1414-431X20132626 |
identifier_str_mv |
ALVES, J. S. M. et al. Limb immobilization alters functional electrophysiological parameters of sciatic nerve. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 46, n. 8, p. 715-721, ago. 2013. DOI: https://doi.org/10.1590/1414-431X20132626. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000800715&lng=en&nrm=iso. Acesso em: 04 dez. 2020. |
url |
http://repositorio.unb.br/handle/10482/28739 https://dx.doi.org/10.1590/1414-431X20132626 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
instname_str |
Universidade de Brasília (UnB) |
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UNB |
institution |
UNB |
reponame_str |
Repositório Institucional da UnB |
collection |
Repositório Institucional da UnB |
repository.name.fl_str_mv |
Repositório Institucional da UnB - Universidade de Brasília (UnB) |
repository.mail.fl_str_mv |
repositorio@unb.br |
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1810580908868108288 |