Production of Brazilian human norovirus VLPs and comparison of purification methods

Detalhes bibliográficos
Autor(a) principal: Lamounier, Thaís Alves da Costa
Data de Publicação: 2015
Outros Autores: Oliveira, Layssa Miranda de, Camargo, Brenda Rabello de, Rodrigues, Kelly Barreto, Noronha, Eliane Ferreira, Ribeiro, Bergmann Morais, Nagata, Tatsuya
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/29849
http://dx.doi.org/10.1590/S1517-838246420140925
Resumo: Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
id UNB_d598a9fd0ab4fd38956d196957beec30
oai_identifier_str oai:repositorio.unb.br:10482/29849
network_acronym_str UNB
network_name_str Repositório Institucional da UnB
repository_id_str
spelling Production of Brazilian human norovirus VLPs and comparison of purification methodsVírusCromatografia de troca iônicaNoroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.Sociedade Brasileira de Microbiologia2017-12-07T05:13:27Z2017-12-07T05:13:27Z2015-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfLAMOUNIER, Thais Alves da Costa et al. Production of Brazilian human norovirus VLPs and comparison of purification methods. Brazilian Journal of Microbiology, São Paulo, v. 46, n. 4, p. 1265-1268, out./dez. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso>. Acesso em: 2 abr. 2018. Epub Oct 09, 2015. doi: http://dx.doi.org/10.1590/S1517-838246420140925.http://repositorio.unb.br/handle/10482/29849http://dx.doi.org/10.1590/S1517-838246420140925Brazilian Journal of Microbiology - All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC-BY (CC BY NC 4.0). Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso. Acesso em: 2 abr. 2018.info:eu-repo/semantics/openAccessLamounier, Thaís Alves da CostaOliveira, Layssa Miranda deCamargo, Brenda Rabello deRodrigues, Kelly BarretoNoronha, Eliane FerreiraRibeiro, Bergmann MoraisNagata, Tatsuyaengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-05-24T23:34:34Zoai:repositorio.unb.br:10482/29849Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:34:34Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Production of Brazilian human norovirus VLPs and comparison of purification methods
title Production of Brazilian human norovirus VLPs and comparison of purification methods
spellingShingle Production of Brazilian human norovirus VLPs and comparison of purification methods
Lamounier, Thaís Alves da Costa
Vírus
Cromatografia de troca iônica
title_short Production of Brazilian human norovirus VLPs and comparison of purification methods
title_full Production of Brazilian human norovirus VLPs and comparison of purification methods
title_fullStr Production of Brazilian human norovirus VLPs and comparison of purification methods
title_full_unstemmed Production of Brazilian human norovirus VLPs and comparison of purification methods
title_sort Production of Brazilian human norovirus VLPs and comparison of purification methods
author Lamounier, Thaís Alves da Costa
author_facet Lamounier, Thaís Alves da Costa
Oliveira, Layssa Miranda de
Camargo, Brenda Rabello de
Rodrigues, Kelly Barreto
Noronha, Eliane Ferreira
Ribeiro, Bergmann Morais
Nagata, Tatsuya
author_role author
author2 Oliveira, Layssa Miranda de
Camargo, Brenda Rabello de
Rodrigues, Kelly Barreto
Noronha, Eliane Ferreira
Ribeiro, Bergmann Morais
Nagata, Tatsuya
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lamounier, Thaís Alves da Costa
Oliveira, Layssa Miranda de
Camargo, Brenda Rabello de
Rodrigues, Kelly Barreto
Noronha, Eliane Ferreira
Ribeiro, Bergmann Morais
Nagata, Tatsuya
dc.subject.por.fl_str_mv Vírus
Cromatografia de troca iônica
topic Vírus
Cromatografia de troca iônica
description Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
publishDate 2015
dc.date.none.fl_str_mv 2015-10
2017-12-07T05:13:27Z
2017-12-07T05:13:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv LAMOUNIER, Thais Alves da Costa et al. Production of Brazilian human norovirus VLPs and comparison of purification methods. Brazilian Journal of Microbiology, São Paulo, v. 46, n. 4, p. 1265-1268, out./dez. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso>. Acesso em: 2 abr. 2018. Epub Oct 09, 2015. doi: http://dx.doi.org/10.1590/S1517-838246420140925.
http://repositorio.unb.br/handle/10482/29849
http://dx.doi.org/10.1590/S1517-838246420140925
identifier_str_mv LAMOUNIER, Thais Alves da Costa et al. Production of Brazilian human norovirus VLPs and comparison of purification methods. Brazilian Journal of Microbiology, São Paulo, v. 46, n. 4, p. 1265-1268, out./dez. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265&lng=en&nrm=iso>. Acesso em: 2 abr. 2018. Epub Oct 09, 2015. doi: http://dx.doi.org/10.1590/S1517-838246420140925.
url http://repositorio.unb.br/handle/10482/29849
http://dx.doi.org/10.1590/S1517-838246420140925
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
_version_ 1810580818792284160

Registros relacionados