Production of Brazilian human norovirus VLPs and comparison of purification methods

Detalhes bibliográficos
Autor(a) principal: Lamounier,Thais Alves da Costa
Data de Publicação: 2015
Outros Autores: Oliveira,Layssa Miranda de, Camargo,Brenda Rabello de, Rodrigues,Kelly Barreto, Noronha,Eliane Ferreira, Ribeiro,Bergmann Morais, Nagata,Tatsuya
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265
Resumo: Abstract Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
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spelling Production of Brazilian human norovirus VLPs and comparison of purification methodsNorwalk virusVLPion-exchange chromatographyAbstract Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.Sociedade Brasileira de Microbiologia2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265Brazilian Journal of Microbiology v.46 n.4 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246420140925info:eu-repo/semantics/openAccessLamounier,Thais Alves da CostaOliveira,Layssa Miranda deCamargo,Brenda Rabello deRodrigues,Kelly BarretoNoronha,Eliane FerreiraRibeiro,Bergmann MoraisNagata,Tatsuyaeng2015-12-17T00:00:00Zoai:scielo:S1517-83822015000401265Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-12-17T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Production of Brazilian human norovirus VLPs and comparison of purification methods
title Production of Brazilian human norovirus VLPs and comparison of purification methods
spellingShingle Production of Brazilian human norovirus VLPs and comparison of purification methods
Lamounier,Thais Alves da Costa
Norwalk virus
VLP
ion-exchange chromatography
title_short Production of Brazilian human norovirus VLPs and comparison of purification methods
title_full Production of Brazilian human norovirus VLPs and comparison of purification methods
title_fullStr Production of Brazilian human norovirus VLPs and comparison of purification methods
title_full_unstemmed Production of Brazilian human norovirus VLPs and comparison of purification methods
title_sort Production of Brazilian human norovirus VLPs and comparison of purification methods
author Lamounier,Thais Alves da Costa
author_facet Lamounier,Thais Alves da Costa
Oliveira,Layssa Miranda de
Camargo,Brenda Rabello de
Rodrigues,Kelly Barreto
Noronha,Eliane Ferreira
Ribeiro,Bergmann Morais
Nagata,Tatsuya
author_role author
author2 Oliveira,Layssa Miranda de
Camargo,Brenda Rabello de
Rodrigues,Kelly Barreto
Noronha,Eliane Ferreira
Ribeiro,Bergmann Morais
Nagata,Tatsuya
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lamounier,Thais Alves da Costa
Oliveira,Layssa Miranda de
Camargo,Brenda Rabello de
Rodrigues,Kelly Barreto
Noronha,Eliane Ferreira
Ribeiro,Bergmann Morais
Nagata,Tatsuya
dc.subject.por.fl_str_mv Norwalk virus
VLP
ion-exchange chromatography
topic Norwalk virus
VLP
ion-exchange chromatography
description Abstract Noroviruses (NVs) are responsible for most cases of human nonbacterial gastroenteritis worldwide. Some parameters for the purification of NV virus-like particles (VLPs) such as ease of production and yield were studied for future development of vaccines and diagnostic tools. In this study, VLPs were produced by the expression of the VP1 and VP2 gene cassette of the Brazilian NV isolate, and two purification methods were compared: cesium chloride (CsCl) gradient centrifugation and ion-exchange chromatography (IEC). IEC produced more and purer VLPs of NV compared to CsCl gradient centrifugation.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401265
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-838246420140925
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.46 n.4 2015
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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