Culture of human dental pulp cells at variable times post-tooth extraction

Detalhes bibliográficos
Autor(a) principal: Benício, Daniela Ferreira Araújo
Data de Publicação: 2018
Outros Autores: Pereira, Luciana Oliveira, Silva, Izabel Cristina Rodrigues da, Azevedo, Ricardo Bentes, Bezerra, Ana Cristina Barreto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UnB
Texto Completo: http://repositorio.unb.br/handle/10482/33615
http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003
Resumo: The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.
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spelling Culture of human dental pulp cells at variable times post-tooth extractionCélulas-troncoPolpa dentáriaThe aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2019-01-02T13:54:57Z2019-01-02T13:54:57Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBENÍCIO, Daniela Ferreira Araújo et al. Culture of human dental pulp cells at variable times post-tooth extraction. Brazilian Oral Research, São Paulo, v. 32, e003, 2018. DOI: http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202&lng=en&nrm=iso. Acesso em: 30 jan. 2019. Epub Feb 01, 2018.http://repositorio.unb.br/handle/10482/33615http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003Brazilian Oral Research - (CC BY) - This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202&lng=en&nrm=iso&tlng=en. Acesso em: 30 jan. 2019.info:eu-repo/semantics/openAccessBenício, Daniela Ferreira AraújoPereira, Luciana OliveiraSilva, Izabel Cristina Rodrigues daAzevedo, Ricardo BentesBezerra, Ana Cristina Barretoengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-05-24T23:06:05Zoai:repositorio.unb.br:10482/33615Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:06:05Repositório Institucional da UnB - Universidade de Brasília (UnB)false
dc.title.none.fl_str_mv Culture of human dental pulp cells at variable times post-tooth extraction
title Culture of human dental pulp cells at variable times post-tooth extraction
spellingShingle Culture of human dental pulp cells at variable times post-tooth extraction
Benício, Daniela Ferreira Araújo
Células-tronco
Polpa dentária
title_short Culture of human dental pulp cells at variable times post-tooth extraction
title_full Culture of human dental pulp cells at variable times post-tooth extraction
title_fullStr Culture of human dental pulp cells at variable times post-tooth extraction
title_full_unstemmed Culture of human dental pulp cells at variable times post-tooth extraction
title_sort Culture of human dental pulp cells at variable times post-tooth extraction
author Benício, Daniela Ferreira Araújo
author_facet Benício, Daniela Ferreira Araújo
Pereira, Luciana Oliveira
Silva, Izabel Cristina Rodrigues da
Azevedo, Ricardo Bentes
Bezerra, Ana Cristina Barreto
author_role author
author2 Pereira, Luciana Oliveira
Silva, Izabel Cristina Rodrigues da
Azevedo, Ricardo Bentes
Bezerra, Ana Cristina Barreto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Benício, Daniela Ferreira Araújo
Pereira, Luciana Oliveira
Silva, Izabel Cristina Rodrigues da
Azevedo, Ricardo Bentes
Bezerra, Ana Cristina Barreto
dc.subject.por.fl_str_mv Células-tronco
Polpa dentária
topic Células-tronco
Polpa dentária
description The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019-01-02T13:54:57Z
2019-01-02T13:54:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv BENÍCIO, Daniela Ferreira Araújo et al. Culture of human dental pulp cells at variable times post-tooth extraction. Brazilian Oral Research, São Paulo, v. 32, e003, 2018. DOI: http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202&lng=en&nrm=iso. Acesso em: 30 jan. 2019. Epub Feb 01, 2018.
http://repositorio.unb.br/handle/10482/33615
http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003
identifier_str_mv BENÍCIO, Daniela Ferreira Araújo et al. Culture of human dental pulp cells at variable times post-tooth extraction. Brazilian Oral Research, São Paulo, v. 32, e003, 2018. DOI: http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202&lng=en&nrm=iso. Acesso em: 30 jan. 2019. Epub Feb 01, 2018.
url http://repositorio.unb.br/handle/10482/33615
http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
publisher.none.fl_str_mv Sociedade Brasileira de Pesquisa Odontológica - SBPqO
dc.source.none.fl_str_mv reponame:Repositório Institucional da UnB
instname:Universidade de Brasília (UnB)
instacron:UNB
instname_str Universidade de Brasília (UnB)
instacron_str UNB
institution UNB
reponame_str Repositório Institucional da UnB
collection Repositório Institucional da UnB
repository.name.fl_str_mv Repositório Institucional da UnB - Universidade de Brasília (UnB)
repository.mail.fl_str_mv repositorio@unb.br
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