Culture of human dental pulp cells at variable times post-tooth extraction
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UnB |
Texto Completo: | http://repositorio.unb.br/handle/10482/33615 http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003 |
Resumo: | The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential. |
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Culture of human dental pulp cells at variable times post-tooth extractionCélulas-troncoPolpa dentáriaThe aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential.Sociedade Brasileira de Pesquisa Odontológica - SBPqO2019-01-02T13:54:57Z2019-01-02T13:54:57Z2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfBENÍCIO, Daniela Ferreira Araújo et al. Culture of human dental pulp cells at variable times post-tooth extraction. Brazilian Oral Research, São Paulo, v. 32, e003, 2018. DOI: http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202&lng=en&nrm=iso. Acesso em: 30 jan. 2019. Epub Feb 01, 2018.http://repositorio.unb.br/handle/10482/33615http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003Brazilian Oral Research - (CC BY) - This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Fonte: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202&lng=en&nrm=iso&tlng=en. Acesso em: 30 jan. 2019.info:eu-repo/semantics/openAccessBenício, Daniela Ferreira AraújoPereira, Luciana OliveiraSilva, Izabel Cristina Rodrigues daAzevedo, Ricardo BentesBezerra, Ana Cristina Barretoengreponame:Repositório Institucional da UnBinstname:Universidade de Brasília (UnB)instacron:UNB2023-05-24T23:06:05Zoai:repositorio.unb.br:10482/33615Repositório InstitucionalPUBhttps://repositorio.unb.br/oai/requestrepositorio@unb.bropendoar:2023-05-24T23:06:05Repositório Institucional da UnB - Universidade de Brasília (UnB)false |
dc.title.none.fl_str_mv |
Culture of human dental pulp cells at variable times post-tooth extraction |
title |
Culture of human dental pulp cells at variable times post-tooth extraction |
spellingShingle |
Culture of human dental pulp cells at variable times post-tooth extraction Benício, Daniela Ferreira Araújo Células-tronco Polpa dentária |
title_short |
Culture of human dental pulp cells at variable times post-tooth extraction |
title_full |
Culture of human dental pulp cells at variable times post-tooth extraction |
title_fullStr |
Culture of human dental pulp cells at variable times post-tooth extraction |
title_full_unstemmed |
Culture of human dental pulp cells at variable times post-tooth extraction |
title_sort |
Culture of human dental pulp cells at variable times post-tooth extraction |
author |
Benício, Daniela Ferreira Araújo |
author_facet |
Benício, Daniela Ferreira Araújo Pereira, Luciana Oliveira Silva, Izabel Cristina Rodrigues da Azevedo, Ricardo Bentes Bezerra, Ana Cristina Barreto |
author_role |
author |
author2 |
Pereira, Luciana Oliveira Silva, Izabel Cristina Rodrigues da Azevedo, Ricardo Bentes Bezerra, Ana Cristina Barreto |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Benício, Daniela Ferreira Araújo Pereira, Luciana Oliveira Silva, Izabel Cristina Rodrigues da Azevedo, Ricardo Bentes Bezerra, Ana Cristina Barreto |
dc.subject.por.fl_str_mv |
Células-tronco Polpa dentária |
topic |
Células-tronco Polpa dentária |
description |
The aim of this study was to investigate the viability of human dental pulp cells from extracted teeth kept at standard room temperature and atmospheric pressure for different periods of time. Twenty-one healthy permanent teeth were used. They were divided into five groups according to the expected time from extraction to processing. One group was tested immediately after extraction; the other groups were each tested at one of the following time points: 30 minutes, 1 hour, 2 hours, and 5 hours post-extraction. Cell morphology was analysed by light microscopy; cell proliferation was analysed using MTT assay and by counting the viable cells in a haemocytometer. Similar results were observed in all groups (p < 0.05). A delay of up to five hours for tooth processing and tissue collection does not preclude the establishment of dental pulp cell cultures, affect the morphology of these cells, or reduce their proliferative potential. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2019-01-02T13:54:57Z 2019-01-02T13:54:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BENÍCIO, Daniela Ferreira Araújo et al. Culture of human dental pulp cells at variable times post-tooth extraction. Brazilian Oral Research, São Paulo, v. 32, e003, 2018. DOI: http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202&lng=en&nrm=iso. Acesso em: 30 jan. 2019. Epub Feb 01, 2018. http://repositorio.unb.br/handle/10482/33615 http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003 |
identifier_str_mv |
BENÍCIO, Daniela Ferreira Araújo et al. Culture of human dental pulp cells at variable times post-tooth extraction. Brazilian Oral Research, São Paulo, v. 32, e003, 2018. DOI: http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003. Disponível em: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100202&lng=en&nrm=iso. Acesso em: 30 jan. 2019. Epub Feb 01, 2018. |
url |
http://repositorio.unb.br/handle/10482/33615 http://dx.doi.org/10.1590/1807-3107bor-2018.vol32.0003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
publisher.none.fl_str_mv |
Sociedade Brasileira de Pesquisa Odontológica - SBPqO |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UnB instname:Universidade de Brasília (UnB) instacron:UNB |
instname_str |
Universidade de Brasília (UnB) |
instacron_str |
UNB |
institution |
UNB |
reponame_str |
Repositório Institucional da UnB |
collection |
Repositório Institucional da UnB |
repository.name.fl_str_mv |
Repositório Institucional da UnB - Universidade de Brasília (UnB) |
repository.mail.fl_str_mv |
repositorio@unb.br |
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1814508371564298240 |