Actinobacteria with agricultural biotechnological potential isolated from fermented organic fertilizer (Bokashi)

Detalhes bibliográficos
Autor(a) principal: Santos, Leonardo da Silva
Data de Publicação: 2021
Outros Autores: Souza, Giselle Silva de, Silva, Luana Gomes da, Araújo, Lyslem Riquelem de, Silva, Mirca Melo Rodrigues da, Lopes, Esmeralda Aparecida Porto
Tipo de documento: Artigo
Idioma: por
Título da fonte: Diversitas Journal
Texto Completo: https://diversitasjournal.com.br/diversitas_journal/article/view/1946
Resumo: Bokashi is a type of organic fermented product formed by fungi and bacteria isolated from forests and which coexist synergistically in a liquid fermentative medium enriched with a sugar source. Among these microorganisms, some species of actinobacteria may have biotechnological potential to be used in agriculture. In this sense, the present work aimed to isolate and identify actinobacteria with agricultural biotechnological potential present in fermented organic fertilizer (Bokashi). Bokashi was produced by capturing microorganisms present in the forest soil. Actinobacteria were isolated through successive dilutions of Bokashi samples and grown in culture media Czapeck Modified (MC), Arginine Vitamin Agar (AY*), Inorganic Salt – starch Agar (ISP-4) containing antifungal, and incubated at 30° C for 21 days. Micromorphological analysis and identification was performed using the microculture on slides technique. In all, 22 strains of actinobacteria were isolated and identified, of which 77.27% were from the AY* medium; 13.64% of ISP-4 medium and 9.1% of MC medium. The presumptive identification of the genders showed that the strains were distributed between: 45.45% Nocardia; 36.36% Streptomyces; 9.1% Terrabacter; 4.54% Micromonospora; and 4.54% Mycobacterium.
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spelling Actinobacteria with agricultural biotechnological potential isolated from fermented organic fertilizer (Bokashi)Actinobactérias com potencial biotecnológico agrícola isoladas de adubo orgânico fermentado (Bokashi)Bokashi is a type of organic fermented product formed by fungi and bacteria isolated from forests and which coexist synergistically in a liquid fermentative medium enriched with a sugar source. Among these microorganisms, some species of actinobacteria may have biotechnological potential to be used in agriculture. In this sense, the present work aimed to isolate and identify actinobacteria with agricultural biotechnological potential present in fermented organic fertilizer (Bokashi). Bokashi was produced by capturing microorganisms present in the forest soil. Actinobacteria were isolated through successive dilutions of Bokashi samples and grown in culture media Czapeck Modified (MC), Arginine Vitamin Agar (AY*), Inorganic Salt – starch Agar (ISP-4) containing antifungal, and incubated at 30° C for 21 days. Micromorphological analysis and identification was performed using the microculture on slides technique. In all, 22 strains of actinobacteria were isolated and identified, of which 77.27% were from the AY* medium; 13.64% of ISP-4 medium and 9.1% of MC medium. The presumptive identification of the genders showed that the strains were distributed between: 45.45% Nocardia; 36.36% Streptomyces; 9.1% Terrabacter; 4.54% Micromonospora; and 4.54% Mycobacterium.RESUMO: O Bokashi é um tipo de fermentado orgânico formado por fungos e bactérias isolados de matas e que coexistem sinergicamente em meio líquido fermentativo. Dentre esses microrganismos, algumas espécies de actinobactérias apresentam potencial biotecnológico aplicado na produção de produtos naturais úteis para a agricultura. Neste sentido, o presente trabalho teve como objetivo isolar e identificar actinobactérias com potencial biotecnológico agrícola presentes em adubo orgânico fermentado (Bokashi). O Bokashi foi produzido através da captura de microrganismos presentes no solo de mata. As actinobactérias foram isoladas através de diluições sucessivas de amostras do Bokashi e crescidas nos meios de cultura Czapeck Modificado (MC), Arginina Vitamina Ágar (AY*), Inorganic Salt – starch Ágar (ISP-4) contendo antifúngico e incubadas a 30 °C por 21 dias. A análise micromofológica e identificação foi realizada através da técnica de microcultivo em lâminas. Ao todo, foram isoladas e identificadas 22 cepas de actinobactérias, das quais 77,27% foram provenientes do meio AY*; 13,64% do meio ISP-4 e 9,09% do meio MC. A identificação presuntiva dos gêneros mostrou que as cepas estavam distribuídas entre: 45,45% Nocardia; 36,36% Streptomyces; 9,10% Terrabacter; 4,54% Micromonospora; e 4,54% Mycobacterium. PALAVRAS-CHAVE: Microrganismos Eficazes, Agricultura sustentável, Biotecnologia.   ABSTRACT: Bokashi is a type of organic ferment formed by fungi and bacteria isolated from forests and which coexist synergistically in a liquid fermentative medium. Among these microorganisms, some species of actinobacteria have biotechnological potential applied in the production of natural products useful for agriculture. In thus sense, the present work aimed to isolate and identify actinobacteria with agricultural biotechnological potential present in fermented organic fertilizer (Bokashi). Bokashi was produced by capturing microorganisms present in the forest soil. Actinobacteria were isolated through successive dilutions of Bokashi samples and grown in culture media Czapeck Modified (MC), Arginine Vitamin Agar (AY*), Inorganic Salt – starch Agar (ISP-4) containing antifungal and incubated at 30 °C for 21 days. Micromorphological analysis and identification was performed using the microculture on slides technique. In all, 22 strains of actinobacteria were isolated and identified, of which 77.27% were from the AY* medium; 13.64% of ISP-4 medium and 9.09% of MC medium. The presumptive identification of the genders showed that the strains were distributed between: 45.45% Nocardia; 36.36% Streptomyces; 9.10% Terrabacter; 4.54% Micromonospora; and 4.54% Mycobacterium. KEYWORDS: Effective Microorganisms, Sustainable Agriculture, Biotechnology.Universidade Estadual de Alagoas - Eduneal2021-10-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://diversitasjournal.com.br/diversitas_journal/article/view/194610.48017/dj.v6i4.1946Diversitas Journal; v. 6 n. 4 (2021): Realidades da educação na Pandemia Sars-Covid 19 ; 3866-38812525-521510.48017/dj.v6i4reponame:Diversitas Journalinstname:Universidade Estadual de Alagoas (UNEAL)instacron:UNEALporhttps://diversitasjournal.com.br/diversitas_journal/article/view/1946/1513Copyright (c) 2021 Santos, L. S., Souza, G. S., Silva, L. G., Araújo, L. R., Silva, M. M. R., Lopes. E. A. P.https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSantos, Leonardo da SilvaSouza, Giselle Silva deSilva, Luana Gomes daAraújo, Lyslem Riquelem deSilva, Mirca Melo Rodrigues daLopes, Esmeralda Aparecida Porto2021-10-24T20:15:52Zoai:ojs.emnuvens.com.br:article/1946Revistahttps://diversitasjournal.com.br/diversitas_journal/indexPUBhttps://www.e-publicacoes.uerj.br/index.php/muralinternacional/oairevistadiversitasjournal@gmail.com2525-52152525-5215opendoar:2023-01-13T09:47:25.454883Diversitas Journal - Universidade Estadual de Alagoas (UNEAL)false
dc.title.none.fl_str_mv Actinobacteria with agricultural biotechnological potential isolated from fermented organic fertilizer (Bokashi)
Actinobactérias com potencial biotecnológico agrícola isoladas de adubo orgânico fermentado (Bokashi)
title Actinobacteria with agricultural biotechnological potential isolated from fermented organic fertilizer (Bokashi)
spellingShingle Actinobacteria with agricultural biotechnological potential isolated from fermented organic fertilizer (Bokashi)
Santos, Leonardo da Silva
title_short Actinobacteria with agricultural biotechnological potential isolated from fermented organic fertilizer (Bokashi)
title_full Actinobacteria with agricultural biotechnological potential isolated from fermented organic fertilizer (Bokashi)
title_fullStr Actinobacteria with agricultural biotechnological potential isolated from fermented organic fertilizer (Bokashi)
title_full_unstemmed Actinobacteria with agricultural biotechnological potential isolated from fermented organic fertilizer (Bokashi)
title_sort Actinobacteria with agricultural biotechnological potential isolated from fermented organic fertilizer (Bokashi)
author Santos, Leonardo da Silva
author_facet Santos, Leonardo da Silva
Souza, Giselle Silva de
Silva, Luana Gomes da
Araújo, Lyslem Riquelem de
Silva, Mirca Melo Rodrigues da
Lopes, Esmeralda Aparecida Porto
author_role author
author2 Souza, Giselle Silva de
Silva, Luana Gomes da
Araújo, Lyslem Riquelem de
Silva, Mirca Melo Rodrigues da
Lopes, Esmeralda Aparecida Porto
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Leonardo da Silva
Souza, Giselle Silva de
Silva, Luana Gomes da
Araújo, Lyslem Riquelem de
Silva, Mirca Melo Rodrigues da
Lopes, Esmeralda Aparecida Porto
description Bokashi is a type of organic fermented product formed by fungi and bacteria isolated from forests and which coexist synergistically in a liquid fermentative medium enriched with a sugar source. Among these microorganisms, some species of actinobacteria may have biotechnological potential to be used in agriculture. In this sense, the present work aimed to isolate and identify actinobacteria with agricultural biotechnological potential present in fermented organic fertilizer (Bokashi). Bokashi was produced by capturing microorganisms present in the forest soil. Actinobacteria were isolated through successive dilutions of Bokashi samples and grown in culture media Czapeck Modified (MC), Arginine Vitamin Agar (AY*), Inorganic Salt – starch Agar (ISP-4) containing antifungal, and incubated at 30° C for 21 days. Micromorphological analysis and identification was performed using the microculture on slides technique. In all, 22 strains of actinobacteria were isolated and identified, of which 77.27% were from the AY* medium; 13.64% of ISP-4 medium and 9.1% of MC medium. The presumptive identification of the genders showed that the strains were distributed between: 45.45% Nocardia; 36.36% Streptomyces; 9.1% Terrabacter; 4.54% Micromonospora; and 4.54% Mycobacterium.
publishDate 2021
dc.date.none.fl_str_mv 2021-10-19
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://diversitasjournal.com.br/diversitas_journal/article/view/1946
10.48017/dj.v6i4.1946
url https://diversitasjournal.com.br/diversitas_journal/article/view/1946
identifier_str_mv 10.48017/dj.v6i4.1946
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://diversitasjournal.com.br/diversitas_journal/article/view/1946/1513
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual de Alagoas - Eduneal
publisher.none.fl_str_mv Universidade Estadual de Alagoas - Eduneal
dc.source.none.fl_str_mv Diversitas Journal; v. 6 n. 4 (2021): Realidades da educação na Pandemia Sars-Covid 19 ; 3866-3881
2525-5215
10.48017/dj.v6i4
reponame:Diversitas Journal
instname:Universidade Estadual de Alagoas (UNEAL)
instacron:UNEAL
instname_str Universidade Estadual de Alagoas (UNEAL)
instacron_str UNEAL
institution UNEAL
reponame_str Diversitas Journal
collection Diversitas Journal
repository.name.fl_str_mv Diversitas Journal - Universidade Estadual de Alagoas (UNEAL)
repository.mail.fl_str_mv revistadiversitasjournal@gmail.com
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