Efeito da inibição da expressão da srebp-1c na reversão da doença do fígado gorduroso induzido por dieta hiperlipídica

Detalhes bibliográficos
Autor(a) principal: Cesconetto, Patricia Acordi
Data de Publicação: 2012
Idioma: por
Título da fonte: Repositório Institucional da UNESC
Texto Completo: http://repositorio.unesc.net/handle/1/1969
Resumo: Trabalho de Conclusão de Curso, apresentado para obtenção do grau de Bacharel no curso de Farmácia da Universidade do Extremo Sul Catarinense, UNESC.
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spelling Cesconetto, Patricia AcordiSouza, Claudio Teodoro deUniversidade do Extremo Sul Catarinense2013-11-18T23:39:34Z2013-11-18T23:39:34Z2012-122013-11-18http://repositorio.unesc.net/handle/1/1969Trabalho de Conclusão de Curso, apresentado para obtenção do grau de Bacharel no curso de Farmácia da Universidade do Extremo Sul Catarinense, UNESC.Objective. The present study investigated the level of Sterol regulatory element binding proteins (SREBP-1c) and related proteins in obese mice treated with SREBP-1c oligonucleotide to observe a reversal of steatosis. Materials and methods. Swiss mice were fed on chow containing 61 kJ% saturated fat for 8 weeks to develop obesity. After this period, one group of animals was used to assess the molecular effects of SREBP-1c antisense oligonucleotide treatment by immunoblot analysis in a dose–response curve (0; 1.0; 2.0; 3.0; 4.0 nmol/day). After the dose (3.0 nmol/day) was determined, another group was treated for 14 days. Twenty-four hours after the last injection mice were killed and plasma and hepatic tissue were obtained to evaluate plasma triglycerides and total liver fat. Samples were blotted with anti-SREBP-1c, FAS, SCD-1, PPAR_, ACC[Ser79] phosphorylation, CPT1, and [Thr172]-phosphorylation of AMPK antibodies. Livers were stained using the haematoxylin method for histological analysis. Results. Body weight, epididymal fat and glucose levels were not affected by one daily dose of SREBP-1c/AS. However, total plasma triglycerides and total liver fat were significantly reduced. Also, this treatment inhibited SREBP-1c and reduced protein levels of a series of proteins involved in lipogenesis, including ACC, FAS, SCD-1. Moreover, mice treated with SREBP-1c/AS presented a significant reduction in macroscopic and microscopic features of hepatic steatosis. Conclusion. Our results demonstrate that the inhibition of SREBP-1c decreased the expression of lipogenic enzymes, reducing the accumulation of triglycerides and, finally, reversing hepatic steatosis. Thus, SREBP-1c is an attractive target for pharmacological therapeutics in fatty liver.ObesidadeDieta hiperlipídicaDoenças do fígadoEfeito da inibição da expressão da srebp-1c na reversão da doença do fígado gorduroso induzido por dieta hiperlipídicainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisporreponame:Repositório Institucional da UNESCinstname:Universidade do Extremo Sul Catarinense (Unesc)instacron:UNESCinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-8894http://repositorio.unesc.net/bitstream/1/1969/2/license.txt09af850207095721090e50e21d50d1efMD52ORIGINALPatrícia Acordi Cesconetto.pdfPatrícia Acordi Cesconetto.pdfArtigoapplication/pdf3189426http://repositorio.unesc.net/bitstream/1/1969/3/Patr%c3%adcia%20Acordi%20Cesconetto.pdfe1ad3d1ec1c459b819ac785346c78e42MD53TEXTPatrícia Acordi Cesconetto.pdf.txtPatrícia Acordi Cesconetto.pdf.txtExtracted texttext/plain103456http://repositorio.unesc.net/bitstream/1/1969/4/Patr%c3%adcia%20Acordi%20Cesconetto.pdf.txt629c2269bfdc88c04d26188ebaf9a7daMD54THUMBNAILPatrícia Acordi Cesconetto.pdf.jpgPatrícia Acordi Cesconetto.pdf.jpgGenerated Thumbnailimage/jpeg1322http://repositorio.unesc.net/bitstream/1/1969/5/Patr%c3%adcia%20Acordi%20Cesconetto.pdf.jpg208e1a5839a88018661c6521164f5540MD551/19692015-08-24 23:15:19.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ório de Publicaçõeshttp://repositorio.unesc.net/
dc.title.pt_BR.fl_str_mv Efeito da inibição da expressão da srebp-1c na reversão da doença do fígado gorduroso induzido por dieta hiperlipídica
title Efeito da inibição da expressão da srebp-1c na reversão da doença do fígado gorduroso induzido por dieta hiperlipídica
spellingShingle Efeito da inibição da expressão da srebp-1c na reversão da doença do fígado gorduroso induzido por dieta hiperlipídica
Cesconetto, Patricia Acordi
Obesidade
Dieta hiperlipídica
Doenças do fígado
title_short Efeito da inibição da expressão da srebp-1c na reversão da doença do fígado gorduroso induzido por dieta hiperlipídica
title_full Efeito da inibição da expressão da srebp-1c na reversão da doença do fígado gorduroso induzido por dieta hiperlipídica
title_fullStr Efeito da inibição da expressão da srebp-1c na reversão da doença do fígado gorduroso induzido por dieta hiperlipídica
title_full_unstemmed Efeito da inibição da expressão da srebp-1c na reversão da doença do fígado gorduroso induzido por dieta hiperlipídica
title_sort Efeito da inibição da expressão da srebp-1c na reversão da doença do fígado gorduroso induzido por dieta hiperlipídica
author Cesconetto, Patricia Acordi
author_facet Cesconetto, Patricia Acordi
author_role author
dc.contributor.author.fl_str_mv Cesconetto, Patricia Acordi
dc.contributor.advisor1.fl_str_mv Souza, Claudio Teodoro de
contributor_str_mv Souza, Claudio Teodoro de
dc.subject.por.fl_str_mv Obesidade
Dieta hiperlipídica
Doenças do fígado
topic Obesidade
Dieta hiperlipídica
Doenças do fígado
dc.description.pt_BR.fl_txt_mv Trabalho de Conclusão de Curso, apresentado para obtenção do grau de Bacharel no curso de Farmácia da Universidade do Extremo Sul Catarinense, UNESC.
dc.description.abstract.por.fl_txt_mv Objective. The present study investigated the level of Sterol regulatory element binding proteins (SREBP-1c) and related proteins in obese mice treated with SREBP-1c oligonucleotide to observe a reversal of steatosis. Materials and methods. Swiss mice were fed on chow containing 61 kJ% saturated fat for 8 weeks to develop obesity. After this period, one group of animals was used to assess the molecular effects of SREBP-1c antisense oligonucleotide treatment by immunoblot analysis in a dose–response curve (0; 1.0; 2.0; 3.0; 4.0 nmol/day). After the dose (3.0 nmol/day) was determined, another group was treated for 14 days. Twenty-four hours after the last injection mice were killed and plasma and hepatic tissue were obtained to evaluate plasma triglycerides and total liver fat. Samples were blotted with anti-SREBP-1c, FAS, SCD-1, PPAR_, ACC[Ser79] phosphorylation, CPT1, and [Thr172]-phosphorylation of AMPK antibodies. Livers were stained using the haematoxylin method for histological analysis. Results. Body weight, epididymal fat and glucose levels were not affected by one daily dose of SREBP-1c/AS. However, total plasma triglycerides and total liver fat were significantly reduced. Also, this treatment inhibited SREBP-1c and reduced protein levels of a series of proteins involved in lipogenesis, including ACC, FAS, SCD-1. Moreover, mice treated with SREBP-1c/AS presented a significant reduction in macroscopic and microscopic features of hepatic steatosis. Conclusion. Our results demonstrate that the inhibition of SREBP-1c decreased the expression of lipogenic enzymes, reducing the accumulation of triglycerides and, finally, reversing hepatic steatosis. Thus, SREBP-1c is an attractive target for pharmacological therapeutics in fatty liver.
description Trabalho de Conclusão de Curso, apresentado para obtenção do grau de Bacharel no curso de Farmácia da Universidade do Extremo Sul Catarinense, UNESC.
publishDate 2012
dc.date.created.fl_str_mv 2012-12
dc.date.accessioned.fl_str_mv 2013-11-18T23:39:34Z
dc.date.available.fl_str_mv 2013-11-18T23:39:34Z
dc.date.issued.fl_str_mv 2013-11-18
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.unesc.net/handle/1/1969
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dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.coverage.spatial.pt_BR.fl_str_mv Universidade do Extremo Sul Catarinense
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNESC
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