Detection of bovine Clostridium perfringens by polymerase chain reaction

Detalhes bibliográficos
Autor(a) principal: Piatti,R. M.
Data de Publicação: 2004
Outros Autores: Ikuno,A. A., Baldassi,L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: The Journal of venomous animals and toxins including tropical diseases (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200005
Resumo: A polymerase chain reaction (PCR) assay to detect Clostridium perfringens alpha toxin gene (cpa) was used to identify eighty-nine C. perfringens strains obtained from bovine clinical material. The strains were biochemically characterized as C. perfringens. The isolated strains were cultured on plates containing brain heart infusion agar with 5% sheep blood under anaerobic conditions. DNA extraction was performed by boiling. The 324 bp amplification product of cpa was observed in all isolates. C. sordellii, C. botulinum, C. novyi, and C. septicum were also tested but did not produce any alpha toxin gene amplification. These findings suggest that PCR is a useful assay in identifying C. perfringens toxin types.
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spelling Detection of bovine Clostridium perfringens by polymerase chain reactionClostridium perfringensPCRalpha toxinpolymerase chain reactionA polymerase chain reaction (PCR) assay to detect Clostridium perfringens alpha toxin gene (cpa) was used to identify eighty-nine C. perfringens strains obtained from bovine clinical material. The strains were biochemically characterized as C. perfringens. The isolated strains were cultured on plates containing brain heart infusion agar with 5% sheep blood under anaerobic conditions. DNA extraction was performed by boiling. The 324 bp amplification product of cpa was observed in all isolates. C. sordellii, C. botulinum, C. novyi, and C. septicum were also tested but did not produce any alpha toxin gene amplification. These findings suggest that PCR is a useful assay in identifying C. perfringens toxin types.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200005Journal of Venomous Animals and Toxins including Tropical Diseases v.10 n.2 2004reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992004000200005info:eu-repo/semantics/openAccessPiatti,R. M.Ikuno,A. A.Baldassi,L.eng2004-06-30T00:00:00Zoai:scielo:S1678-91992004000200005Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2004-06-30T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection of bovine Clostridium perfringens by polymerase chain reaction
title Detection of bovine Clostridium perfringens by polymerase chain reaction
spellingShingle Detection of bovine Clostridium perfringens by polymerase chain reaction
Piatti,R. M.
Clostridium perfringens
PCR
alpha toxin
polymerase chain reaction
title_short Detection of bovine Clostridium perfringens by polymerase chain reaction
title_full Detection of bovine Clostridium perfringens by polymerase chain reaction
title_fullStr Detection of bovine Clostridium perfringens by polymerase chain reaction
title_full_unstemmed Detection of bovine Clostridium perfringens by polymerase chain reaction
title_sort Detection of bovine Clostridium perfringens by polymerase chain reaction
author Piatti,R. M.
author_facet Piatti,R. M.
Ikuno,A. A.
Baldassi,L.
author_role author
author2 Ikuno,A. A.
Baldassi,L.
author2_role author
author
dc.contributor.author.fl_str_mv Piatti,R. M.
Ikuno,A. A.
Baldassi,L.
dc.subject.por.fl_str_mv Clostridium perfringens
PCR
alpha toxin
polymerase chain reaction
topic Clostridium perfringens
PCR
alpha toxin
polymerase chain reaction
description A polymerase chain reaction (PCR) assay to detect Clostridium perfringens alpha toxin gene (cpa) was used to identify eighty-nine C. perfringens strains obtained from bovine clinical material. The strains were biochemically characterized as C. perfringens. The isolated strains were cultured on plates containing brain heart infusion agar with 5% sheep blood under anaerobic conditions. DNA extraction was performed by boiling. The 324 bp amplification product of cpa was observed in all isolates. C. sordellii, C. botulinum, C. novyi, and C. septicum were also tested but did not produce any alpha toxin gene amplification. These findings suggest that PCR is a useful assay in identifying C. perfringens toxin types.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000200005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-91992004000200005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
dc.source.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases v.10 n.2 2004
reponame:The Journal of venomous animals and toxins including tropical diseases (Online)
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str The Journal of venomous animals and toxins including tropical diseases (Online)
collection The Journal of venomous animals and toxins including tropical diseases (Online)
repository.name.fl_str_mv The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv ||editorial@jvat.org.br
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