Standardization of anti-lethal toxin potency test of antivenoms prepared from two different Agkistrodon halys venoms
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | The Journal of venomous animals and toxins including tropical diseases (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400004 |
Resumo: | In Korea, antivenoms for the treatment of patients bitten by venomous snakes have been imported from Japan or China. Although there is cross-reactivity between these antibodies and venoms from snakes indigenous to Korea (e.g. Agkistrodon genus), protection is not optimal. Antivenoms specifically prepared to neutralize Korean snake venoms could be more effective, with fewer side effects. To this end, we established an infrastructure to develop national standards and created a standardized method to evaluate the efficacy of two horse-derived antivenoms using mouse lethal toxin test. Additionally, we determined the antivenoms neutralizing activity against lethal doses (LD50) of Agkistrodon halys (from Japan) and Jiangzhe Agkistrodon halys (from China) venoms. We also performed cross-neutralization tests using probit analysis on each pairing of venom and antivenom in order to check the possibility of using Jiangzhe A. halys venom as a substitute for A. halys venom, the current standard. Slope of A. halys venom with A. halys antivenom was 10.2 and that of A. halys venom with Jiangzhe A. halys antivenom was 9.6. However, Slope of Jiangzhe A. halys venom with A. halys antivenom was 4.7 while that of Jiangzhe A. halys venom with Jiangzhe A. halys antivenom was 11.5. Therefore, the significant difference in slope patterns suggests that Jiangzhe A. halys venom cannot be used as a substitute for the standard venom to test the anti-lethal toxin activity of antivenoms (p<0.05). |
id |
UNESP-11_5d3b2ac9fc5abaf4c3181bde53d6c4c0 |
---|---|
oai_identifier_str |
oai:scielo:S1678-91992006000400004 |
network_acronym_str |
UNESP-11 |
network_name_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository_id_str |
|
spelling |
Standardization of anti-lethal toxin potency test of antivenoms prepared from two different Agkistrodon halys venomsanti-lethal toxin potencyJiangzhe Angkistrodon halyslethal activityprobit analysisslopeIn Korea, antivenoms for the treatment of patients bitten by venomous snakes have been imported from Japan or China. Although there is cross-reactivity between these antibodies and venoms from snakes indigenous to Korea (e.g. Agkistrodon genus), protection is not optimal. Antivenoms specifically prepared to neutralize Korean snake venoms could be more effective, with fewer side effects. To this end, we established an infrastructure to develop national standards and created a standardized method to evaluate the efficacy of two horse-derived antivenoms using mouse lethal toxin test. Additionally, we determined the antivenoms neutralizing activity against lethal doses (LD50) of Agkistrodon halys (from Japan) and Jiangzhe Agkistrodon halys (from China) venoms. We also performed cross-neutralization tests using probit analysis on each pairing of venom and antivenom in order to check the possibility of using Jiangzhe A. halys venom as a substitute for A. halys venom, the current standard. Slope of A. halys venom with A. halys antivenom was 10.2 and that of A. halys venom with Jiangzhe A. halys antivenom was 9.6. However, Slope of Jiangzhe A. halys venom with A. halys antivenom was 4.7 while that of Jiangzhe A. halys venom with Jiangzhe A. halys antivenom was 11.5. Therefore, the significant difference in slope patterns suggests that Jiangzhe A. halys venom cannot be used as a substitute for the standard venom to test the anti-lethal toxin activity of antivenoms (p<0.05).Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400004Journal of Venomous Animals and Toxins including Tropical Diseases v.12 n.4 2006reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992006000400004info:eu-repo/semantics/openAccessLee,K. H.Won,H. J.Kim,S. N.Yoo,S. H.Shin,I. S.Shin,K. H.Hong,S. H.Lee,S. H.Min,H. K.Park,S. N.Hur,S. J.eng2007-01-11T00:00:00Zoai:scielo:S1678-91992006000400004Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2007-01-11T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Standardization of anti-lethal toxin potency test of antivenoms prepared from two different Agkistrodon halys venoms |
title |
Standardization of anti-lethal toxin potency test of antivenoms prepared from two different Agkistrodon halys venoms |
spellingShingle |
Standardization of anti-lethal toxin potency test of antivenoms prepared from two different Agkistrodon halys venoms Lee,K. H. anti-lethal toxin potency Jiangzhe Angkistrodon halys lethal activity probit analysis slope |
title_short |
Standardization of anti-lethal toxin potency test of antivenoms prepared from two different Agkistrodon halys venoms |
title_full |
Standardization of anti-lethal toxin potency test of antivenoms prepared from two different Agkistrodon halys venoms |
title_fullStr |
Standardization of anti-lethal toxin potency test of antivenoms prepared from two different Agkistrodon halys venoms |
title_full_unstemmed |
Standardization of anti-lethal toxin potency test of antivenoms prepared from two different Agkistrodon halys venoms |
title_sort |
Standardization of anti-lethal toxin potency test of antivenoms prepared from two different Agkistrodon halys venoms |
author |
Lee,K. H. |
author_facet |
Lee,K. H. Won,H. J. Kim,S. N. Yoo,S. H. Shin,I. S. Shin,K. H. Hong,S. H. Lee,S. H. Min,H. K. Park,S. N. Hur,S. J. |
author_role |
author |
author2 |
Won,H. J. Kim,S. N. Yoo,S. H. Shin,I. S. Shin,K. H. Hong,S. H. Lee,S. H. Min,H. K. Park,S. N. Hur,S. J. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lee,K. H. Won,H. J. Kim,S. N. Yoo,S. H. Shin,I. S. Shin,K. H. Hong,S. H. Lee,S. H. Min,H. K. Park,S. N. Hur,S. J. |
dc.subject.por.fl_str_mv |
anti-lethal toxin potency Jiangzhe Angkistrodon halys lethal activity probit analysis slope |
topic |
anti-lethal toxin potency Jiangzhe Angkistrodon halys lethal activity probit analysis slope |
description |
In Korea, antivenoms for the treatment of patients bitten by venomous snakes have been imported from Japan or China. Although there is cross-reactivity between these antibodies and venoms from snakes indigenous to Korea (e.g. Agkistrodon genus), protection is not optimal. Antivenoms specifically prepared to neutralize Korean snake venoms could be more effective, with fewer side effects. To this end, we established an infrastructure to develop national standards and created a standardized method to evaluate the efficacy of two horse-derived antivenoms using mouse lethal toxin test. Additionally, we determined the antivenoms neutralizing activity against lethal doses (LD50) of Agkistrodon halys (from Japan) and Jiangzhe Agkistrodon halys (from China) venoms. We also performed cross-neutralization tests using probit analysis on each pairing of venom and antivenom in order to check the possibility of using Jiangzhe A. halys venom as a substitute for A. halys venom, the current standard. Slope of A. halys venom with A. halys antivenom was 10.2 and that of A. halys venom with Jiangzhe A. halys antivenom was 9.6. However, Slope of Jiangzhe A. halys venom with A. halys antivenom was 4.7 while that of Jiangzhe A. halys venom with Jiangzhe A. halys antivenom was 11.5. Therefore, the significant difference in slope patterns suggests that Jiangzhe A. halys venom cannot be used as a substitute for the standard venom to test the anti-lethal toxin activity of antivenoms (p<0.05). |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000400004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1678-91992006000400004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases v.12 n.4 2006 reponame:The Journal of venomous animals and toxins including tropical diseases (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
collection |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository.name.fl_str_mv |
The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||editorial@jvat.org.br |
_version_ |
1748958537922379776 |