Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys

Detalhes bibliográficos
Autor(a) principal: Ghorbanpur,M
Data de Publicação: 2010
Outros Autores: Zare Mirakabadi,A, Zokaee,F, Zolfagarrian,H
Tipo de documento: Artigo
Idioma: eng
Título da fonte: The Journal of venomous animals and toxins including tropical diseases (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100010
Resumo: An anticoagulant factor was purified from the venom of the Iranian snake Agkistrodon halys by gel filtration on Sephadex G-50 and ion-exchange chromatography on DEAE-Sepharose. In the final stage of purification, the percentage recovery of purified anticoagulant factor was found to be 83%. The purified anticoagulant factor revealed a single protein band in SDS-polyacrylamide electrophoresis under reducing conditions and its molecular weight was about 22 kDa. The purified peptide did not show any effect on casein, BApNA or plasma.
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spelling Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halyssnake venomAgkistrodon halysanticoagulant factorchromatographyAn anticoagulant factor was purified from the venom of the Iranian snake Agkistrodon halys by gel filtration on Sephadex G-50 and ion-exchange chromatography on DEAE-Sepharose. In the final stage of purification, the percentage recovery of purified anticoagulant factor was found to be 83%. The purified anticoagulant factor revealed a single protein band in SDS-polyacrylamide electrophoresis under reducing conditions and its molecular weight was about 22 kDa. The purified peptide did not show any effect on casein, BApNA or plasma.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100010Journal of Venomous Animals and Toxins including Tropical Diseases v.16 n.1 2010reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992010005000005info:eu-repo/semantics/openAccessGhorbanpur,MZare Mirakabadi,AZokaee,FZolfagarrian,Heng2010-03-18T00:00:00Zoai:scielo:S1678-91992010000100010Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2010-03-18T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
title Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
spellingShingle Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
Ghorbanpur,M
snake venom
Agkistrodon halys
anticoagulant factor
chromatography
title_short Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
title_full Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
title_fullStr Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
title_full_unstemmed Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
title_sort Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys
author Ghorbanpur,M
author_facet Ghorbanpur,M
Zare Mirakabadi,A
Zokaee,F
Zolfagarrian,H
author_role author
author2 Zare Mirakabadi,A
Zokaee,F
Zolfagarrian,H
author2_role author
author
author
dc.contributor.author.fl_str_mv Ghorbanpur,M
Zare Mirakabadi,A
Zokaee,F
Zolfagarrian,H
dc.subject.por.fl_str_mv snake venom
Agkistrodon halys
anticoagulant factor
chromatography
topic snake venom
Agkistrodon halys
anticoagulant factor
chromatography
description An anticoagulant factor was purified from the venom of the Iranian snake Agkistrodon halys by gel filtration on Sephadex G-50 and ion-exchange chromatography on DEAE-Sepharose. In the final stage of purification, the percentage recovery of purified anticoagulant factor was found to be 83%. The purified anticoagulant factor revealed a single protein band in SDS-polyacrylamide electrophoresis under reducing conditions and its molecular weight was about 22 kDa. The purified peptide did not show any effect on casein, BApNA or plasma.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100010
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000100010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-91992010005000005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
dc.source.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases v.16 n.1 2010
reponame:The Journal of venomous animals and toxins including tropical diseases (Online)
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str The Journal of venomous animals and toxins including tropical diseases (Online)
collection The Journal of venomous animals and toxins including tropical diseases (Online)
repository.name.fl_str_mv The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv ||editorial@jvat.org.br
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