Relationship between Helicobacter pylori detection and an increased risk of infection in childhood

Detalhes bibliográficos
Autor(a) principal: Venerando,R
Data de Publicação: 2012
Outros Autores: Rasmussen,LT, de Labio,RW, Gatti,LL, Francisco,O, Viani,GA, Rivera,LML, Payão,SLM
Tipo de documento: Artigo
Idioma: eng
Título da fonte: The Journal of venomous animals and toxins including tropical diseases (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400004
Resumo: The risk of developing gastric cancer is believed to be related to differences among Helicobacter pylori strains and the inflammatory responses mediated by host genetic factors. H. pylori infection is acquired at an early age and in the absence of appropriate antibiotic therapy, it generally persists for life. Tp53 gene regulates the transcription of several cytokines and chemokines involved in innate immunity and its action may be influenced by the presence of different H. pylori strains. The present study aimed to detect H. pylori in pediatric patients, to access Tp53 polymorphism at codon 72 and to correlate such findings with age and histopathological results. Three hundred and forty-two patients were analyzed. DNA from their gastric biopsies was extracted and the detection of H. pylori was performed through polymerase chain reaction assays, urease test and histopathologic examination. Allelic discrimination of SNP rs1042522 (Tp53) was performed by real-time polymerase chain reaction. Our results suggest a possible relationship between the presence of H. pylori and chronic gastritis in children and young patients, and showed a significant association between ageing and positivity for H. pylori. It was verified that patients aged < 10 years were 1.3 times more likely to have infection by H. pylori when compared with those aged &gt; 10 years. Finally, no association was found between Tp53 polymorphisms and the presence of H. pylori.
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spelling Relationship between Helicobacter pylori detection and an increased risk of infection in childhoodHelicobacter pylorichildhoodTp53 polymorphimsPCRThe risk of developing gastric cancer is believed to be related to differences among Helicobacter pylori strains and the inflammatory responses mediated by host genetic factors. H. pylori infection is acquired at an early age and in the absence of appropriate antibiotic therapy, it generally persists for life. Tp53 gene regulates the transcription of several cytokines and chemokines involved in innate immunity and its action may be influenced by the presence of different H. pylori strains. The present study aimed to detect H. pylori in pediatric patients, to access Tp53 polymorphism at codon 72 and to correlate such findings with age and histopathological results. Three hundred and forty-two patients were analyzed. DNA from their gastric biopsies was extracted and the detection of H. pylori was performed through polymerase chain reaction assays, urease test and histopathologic examination. Allelic discrimination of SNP rs1042522 (Tp53) was performed by real-time polymerase chain reaction. Our results suggest a possible relationship between the presence of H. pylori and chronic gastritis in children and young patients, and showed a significant association between ageing and positivity for H. pylori. It was verified that patients aged < 10 years were 1.3 times more likely to have infection by H. pylori when compared with those aged &gt; 10 years. Finally, no association was found between Tp53 polymorphisms and the presence of H. pylori.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2012-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400004Journal of Venomous Animals and Toxins including Tropical Diseases v.18 n.4 2012reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992012000400004info:eu-repo/semantics/openAccessVenerando,RRasmussen,LTde Labio,RWGatti,LLFrancisco,OViani,GARivera,LMLPayão,SLMeng2012-12-07T00:00:00Zoai:scielo:S1678-91992012000400004Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2012-12-07T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Relationship between Helicobacter pylori detection and an increased risk of infection in childhood
title Relationship between Helicobacter pylori detection and an increased risk of infection in childhood
spellingShingle Relationship between Helicobacter pylori detection and an increased risk of infection in childhood
Venerando,R
Helicobacter pylori
childhood
Tp53 polymorphims
PCR
title_short Relationship between Helicobacter pylori detection and an increased risk of infection in childhood
title_full Relationship between Helicobacter pylori detection and an increased risk of infection in childhood
title_fullStr Relationship between Helicobacter pylori detection and an increased risk of infection in childhood
title_full_unstemmed Relationship between Helicobacter pylori detection and an increased risk of infection in childhood
title_sort Relationship between Helicobacter pylori detection and an increased risk of infection in childhood
author Venerando,R
author_facet Venerando,R
Rasmussen,LT
de Labio,RW
Gatti,LL
Francisco,O
Viani,GA
Rivera,LML
Payão,SLM
author_role author
author2 Rasmussen,LT
de Labio,RW
Gatti,LL
Francisco,O
Viani,GA
Rivera,LML
Payão,SLM
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Venerando,R
Rasmussen,LT
de Labio,RW
Gatti,LL
Francisco,O
Viani,GA
Rivera,LML
Payão,SLM
dc.subject.por.fl_str_mv Helicobacter pylori
childhood
Tp53 polymorphims
PCR
topic Helicobacter pylori
childhood
Tp53 polymorphims
PCR
description The risk of developing gastric cancer is believed to be related to differences among Helicobacter pylori strains and the inflammatory responses mediated by host genetic factors. H. pylori infection is acquired at an early age and in the absence of appropriate antibiotic therapy, it generally persists for life. Tp53 gene regulates the transcription of several cytokines and chemokines involved in innate immunity and its action may be influenced by the presence of different H. pylori strains. The present study aimed to detect H. pylori in pediatric patients, to access Tp53 polymorphism at codon 72 and to correlate such findings with age and histopathological results. Three hundred and forty-two patients were analyzed. DNA from their gastric biopsies was extracted and the detection of H. pylori was performed through polymerase chain reaction assays, urease test and histopathologic examination. Allelic discrimination of SNP rs1042522 (Tp53) was performed by real-time polymerase chain reaction. Our results suggest a possible relationship between the presence of H. pylori and chronic gastritis in children and young patients, and showed a significant association between ageing and positivity for H. pylori. It was verified that patients aged < 10 years were 1.3 times more likely to have infection by H. pylori when compared with those aged &gt; 10 years. Finally, no association was found between Tp53 polymorphisms and the presence of H. pylori.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000400004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1678-91992012000400004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
publisher.none.fl_str_mv Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)
dc.source.none.fl_str_mv Journal of Venomous Animals and Toxins including Tropical Diseases v.18 n.4 2012
reponame:The Journal of venomous animals and toxins including tropical diseases (Online)
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str The Journal of venomous animals and toxins including tropical diseases (Online)
collection The Journal of venomous animals and toxins including tropical diseases (Online)
repository.name.fl_str_mv The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv ||editorial@jvat.org.br
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