Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | The Journal of venomous animals and toxins including tropical diseases (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300009 |
Resumo: | To establish Korea National Standards for venoms and antivenoms, it is necessary to have standardized assay methods. In this study, we standardized a method to evaluate the antihemorrhagic potency of two horse-derived antivenoms using rabbit intracutaneous injection. We expressed the capability of these antivenoms to neutralize the hemorrhagic activities triggered by the venoms of Agkistrodon halys from Japan and Jiangzhe Agkistrodon halys from China as Minimum Hemorrhagic Dose (MHD). We also performed cross-neutralization tests employing the parallel line assay on different pairings of venoms and antivenoms to check the possibility of using Jiangzhe Agkistrodon halys venom as a substitute for the standard Agkistrodon halys venom in measurements of the antihemorrhagic activity, since A. halys venom is not easily available. Slope function ratio (S.R.) was 0.957 for Agkistrodon halys venom either with Agkistrodon halys antivenom or with Jiangzhe Agkistrodon halys antivenom (p>0.05). Similarly, S.R. was 0.348 for Jiangzhe Agkistrodon halys venom either with Agkistrodon halys antivenom or with Jiangzhe Agkistrodon halys antivenom (p>0.05). Thus, in this study we established antihemorrhagic potency test methods for both Agkistrodon halys and Jiangzhe Agkistrodon halys antivenoms and we could also show it is possible to use Jiangzhe Agkistrodon halys venom as a standard. |
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The Journal of venomous animals and toxins including tropical diseases (Online) |
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Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venomsantihemorrhagic potencyminimum hemorrhagic doseJiangzhe Agkistrodon halysparallel line assayTo establish Korea National Standards for venoms and antivenoms, it is necessary to have standardized assay methods. In this study, we standardized a method to evaluate the antihemorrhagic potency of two horse-derived antivenoms using rabbit intracutaneous injection. We expressed the capability of these antivenoms to neutralize the hemorrhagic activities triggered by the venoms of Agkistrodon halys from Japan and Jiangzhe Agkistrodon halys from China as Minimum Hemorrhagic Dose (MHD). We also performed cross-neutralization tests employing the parallel line assay on different pairings of venoms and antivenoms to check the possibility of using Jiangzhe Agkistrodon halys venom as a substitute for the standard Agkistrodon halys venom in measurements of the antihemorrhagic activity, since A. halys venom is not easily available. Slope function ratio (S.R.) was 0.957 for Agkistrodon halys venom either with Agkistrodon halys antivenom or with Jiangzhe Agkistrodon halys antivenom (p>0.05). Similarly, S.R. was 0.348 for Jiangzhe Agkistrodon halys venom either with Agkistrodon halys antivenom or with Jiangzhe Agkistrodon halys antivenom (p>0.05). Thus, in this study we established antihemorrhagic potency test methods for both Agkistrodon halys and Jiangzhe Agkistrodon halys antivenoms and we could also show it is possible to use Jiangzhe Agkistrodon halys venom as a standard.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300009Journal of Venomous Animals and Toxins including Tropical Diseases v.12 n.3 2006reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1590/S1678-91992006000300009info:eu-repo/semantics/openAccessLee,K. H.Hur,S. J.Kim,S. N.Yoo,S. H.Shin,I. S.Won,H. J.Shin,K. H.Hong,S. H.Lee,S. H.Min,H. K.Park,S. N.eng2006-09-19T00:00:00Zoai:scielo:S1678-91992006000300009Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2006-09-19T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms |
title |
Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms |
spellingShingle |
Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms Lee,K. H. antihemorrhagic potency minimum hemorrhagic dose Jiangzhe Agkistrodon halys parallel line assay |
title_short |
Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms |
title_full |
Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms |
title_fullStr |
Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms |
title_full_unstemmed |
Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms |
title_sort |
Standardization of an antihemorrhagic potency test of antivenoms prepared from two different Agkistrodon halys venoms |
author |
Lee,K. H. |
author_facet |
Lee,K. H. Hur,S. J. Kim,S. N. Yoo,S. H. Shin,I. S. Won,H. J. Shin,K. H. Hong,S. H. Lee,S. H. Min,H. K. Park,S. N. |
author_role |
author |
author2 |
Hur,S. J. Kim,S. N. Yoo,S. H. Shin,I. S. Won,H. J. Shin,K. H. Hong,S. H. Lee,S. H. Min,H. K. Park,S. N. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lee,K. H. Hur,S. J. Kim,S. N. Yoo,S. H. Shin,I. S. Won,H. J. Shin,K. H. Hong,S. H. Lee,S. H. Min,H. K. Park,S. N. |
dc.subject.por.fl_str_mv |
antihemorrhagic potency minimum hemorrhagic dose Jiangzhe Agkistrodon halys parallel line assay |
topic |
antihemorrhagic potency minimum hemorrhagic dose Jiangzhe Agkistrodon halys parallel line assay |
description |
To establish Korea National Standards for venoms and antivenoms, it is necessary to have standardized assay methods. In this study, we standardized a method to evaluate the antihemorrhagic potency of two horse-derived antivenoms using rabbit intracutaneous injection. We expressed the capability of these antivenoms to neutralize the hemorrhagic activities triggered by the venoms of Agkistrodon halys from Japan and Jiangzhe Agkistrodon halys from China as Minimum Hemorrhagic Dose (MHD). We also performed cross-neutralization tests employing the parallel line assay on different pairings of venoms and antivenoms to check the possibility of using Jiangzhe Agkistrodon halys venom as a substitute for the standard Agkistrodon halys venom in measurements of the antihemorrhagic activity, since A. halys venom is not easily available. Slope function ratio (S.R.) was 0.957 for Agkistrodon halys venom either with Agkistrodon halys antivenom or with Jiangzhe Agkistrodon halys antivenom (p>0.05). Similarly, S.R. was 0.348 for Jiangzhe Agkistrodon halys venom either with Agkistrodon halys antivenom or with Jiangzhe Agkistrodon halys antivenom (p>0.05). Thus, in this study we established antihemorrhagic potency test methods for both Agkistrodon halys and Jiangzhe Agkistrodon halys antivenoms and we could also show it is possible to use Jiangzhe Agkistrodon halys venom as a standard. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000300009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1678-91992006000300009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases v.12 n.3 2006 reponame:The Journal of venomous animals and toxins including tropical diseases (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
collection |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository.name.fl_str_mv |
The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||editorial@jvat.org.br |
_version_ |
1748958537910845440 |