Samsum ant venom modulates the immune response and redox status at the acute toxic dose in vivo

• Autor(a) principal: Ebaid,Hossam
• Data de Publicação: 2019
• Outros Autores: Abdel-Salam,Bahaa, Alhazza,Ibrahim, Al-Tamimi,Jameel, Hassan,Iftekhar, Rady,Ahmed, Mashaly,Ashraf, Mahmoud,Ahmed, Sammour,Reda
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: The Journal of venomous animals and toxins including tropical diseases (Online)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100208
• topic: Samsum ant venom; Polymorphonuclear cells (PMNs); Costimulatory molecules (CD80 and CD86); Major histocompatibility complex (MHC); MHC-II; Interferon gamma (INF-γ); Interleukin-17 (IL-17)

Abstract Background: Ant venoms express surface molecules that participate in antigen presentation involving pro- and anti-inflammatory cytokines. This work aims to investigate the expression of MHC-II, CD80 and CD86 on the polymorphonuclear cells (PMNs) in rats injected with samsum ant venom (SAV). Methods: Rats were divided into three groups - control, SAV-treated (intraperitoneal route, 600 μg/kg), and SAV-treated (subcutaneous route, 600 μg/kg). After five doses, animals were euthanized and samples collected for analysis. Results: The subcutaneous SAV-trated rats presented decreased levels of glutathione with increased cholesterol and triglyceride levels. Intraperitoneal SAV-treated animals displayed significantly reduced concentrations of both IFN-γ and IL-17 in comparison with the control group. However, intraperitoneal and subcutaneous SAV-treated rats were able to upregulate the expressions of MHC-II, CD80 and CD86 on PMNs in comparison with the control respectively. The histological examination showed severe lymphocyte depletion in the splenic white pulp of the intraperitoneal SAV-injected rats. Conclusion: Stimulation of PMNs by SAV leads to upregulation of MHC-II, CD 80, and CD 86, which plays critical roles in antigen presentation and consequently proliferation of T-cells. Subcutaneous route was more efficient than intraperitoneal by elevating MHC-II, CD80 and CD86 expression, disturbing oxidative stability and increasing lipogram concentration.