Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | The Journal of venomous animals and toxins including tropical diseases (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200311 |
Resumo: | Background:Calcium channel blockers such as conotoxins have shown a great potential to reduce brain and spinal cord injury. MVIIC neuroprotective effects analyzed in in vitromodels of brain and spinal cord ischemia suggest a potential role of this toxin in preventing injury after spinal cord trauma. However, previous clinical studies with MVIIC demonstrated that clinical side effects might limit the usefulness of this drug and there is no research on its systemic effects. Therefore, the present study aimed to investigate the potential toxic effects of MVIIC on organs and to evaluate clinical and blood profiles of rats submitted to spinal cord injury and treated with this marine toxin. Rats were treated with placebo or MVIIC (at doses of 15, 30, 60 or 120 pmol) intralesionally following spinal cord injury. Seven days after the toxin administration, kidney, brain, lung, heart, liver, adrenal, muscles, pancreas, spleen, stomach, and intestine were histopathologically investigated. In addition, blood samples collected from the rats were tested for any hematologic or biochemical changes.Results:The clinical, hematologic and biochemical evaluation revealed no significant abnormalities in all groups, even in high doses. There was no significant alteration in organs, except for degenerative changes in kidneys at a dose of 120 pmol.Conclusions:These findings suggest that MVIIC at 15, 30 and 60 pmol are safe for intralesional administration after spinal cord injury and could be further investigated in relation to its neuroprotective effects. However, 120 pmol doses of MVIIC may provoke adverse effects on kidney tissue. |
id |
UNESP-11_b8d5d6ccaf8dd74a7bb8d7e2d280137f |
---|---|
oai_identifier_str |
oai:scielo:S1678-91992014000200311 |
network_acronym_str |
UNESP-11 |
network_name_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository_id_str |
|
spelling |
Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in ratsConus magusCone snailHistopathologyHematologyBiochemistryBackground:Calcium channel blockers such as conotoxins have shown a great potential to reduce brain and spinal cord injury. MVIIC neuroprotective effects analyzed in in vitromodels of brain and spinal cord ischemia suggest a potential role of this toxin in preventing injury after spinal cord trauma. However, previous clinical studies with MVIIC demonstrated that clinical side effects might limit the usefulness of this drug and there is no research on its systemic effects. Therefore, the present study aimed to investigate the potential toxic effects of MVIIC on organs and to evaluate clinical and blood profiles of rats submitted to spinal cord injury and treated with this marine toxin. Rats were treated with placebo or MVIIC (at doses of 15, 30, 60 or 120 pmol) intralesionally following spinal cord injury. Seven days after the toxin administration, kidney, brain, lung, heart, liver, adrenal, muscles, pancreas, spleen, stomach, and intestine were histopathologically investigated. In addition, blood samples collected from the rats were tested for any hematologic or biochemical changes.Results:The clinical, hematologic and biochemical evaluation revealed no significant abnormalities in all groups, even in high doses. There was no significant alteration in organs, except for degenerative changes in kidneys at a dose of 120 pmol.Conclusions:These findings suggest that MVIIC at 15, 30 and 60 pmol are safe for intralesional administration after spinal cord injury and could be further investigated in relation to its neuroprotective effects. However, 120 pmol doses of MVIIC may provoke adverse effects on kidney tissue.Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP)2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200311Journal of Venomous Animals and Toxins including Tropical Diseases v.20 2014reponame:The Journal of venomous animals and toxins including tropical diseases (Online)instname:Universidade Estadual Paulista (UNESP)instacron:UNESP10.1186/1678-9199-20-15info:eu-repo/semantics/openAccessOliveira,Karen MSilva,Carla Maria OLavor,Mário Sérgio LRosado,Isabel RFukushima,Fabíola BAssumpção,Anna Luiza FVNeves,Saira MNMotta,Guilherme RGarcia,Fernanda FGomez,Marcus ViníciusMelo,Marília MMelo,Eliane Geng2018-08-17T00:00:00Zoai:scielo:S1678-91992014000200311Revistahttp://www.scielo.br/jvatitdPUBhttps://old.scielo.br/oai/scielo-oai.php||editorial@jvat.org.br1678-91991678-9180opendoar:2018-08-17T00:00The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats |
title |
Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats |
spellingShingle |
Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats Oliveira,Karen M Conus magus Cone snail Histopathology Hematology Biochemistry |
title_short |
Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats |
title_full |
Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats |
title_fullStr |
Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats |
title_full_unstemmed |
Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats |
title_sort |
Systemic effects induced by intralesional injection of ω-conotoxin MVIIC after spinal cord injury in rats |
author |
Oliveira,Karen M |
author_facet |
Oliveira,Karen M Silva,Carla Maria O Lavor,Mário Sérgio L Rosado,Isabel R Fukushima,Fabíola B Assumpção,Anna Luiza FV Neves,Saira MN Motta,Guilherme R Garcia,Fernanda F Gomez,Marcus Vinícius Melo,Marília M Melo,Eliane G |
author_role |
author |
author2 |
Silva,Carla Maria O Lavor,Mário Sérgio L Rosado,Isabel R Fukushima,Fabíola B Assumpção,Anna Luiza FV Neves,Saira MN Motta,Guilherme R Garcia,Fernanda F Gomez,Marcus Vinícius Melo,Marília M Melo,Eliane G |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira,Karen M Silva,Carla Maria O Lavor,Mário Sérgio L Rosado,Isabel R Fukushima,Fabíola B Assumpção,Anna Luiza FV Neves,Saira MN Motta,Guilherme R Garcia,Fernanda F Gomez,Marcus Vinícius Melo,Marília M Melo,Eliane G |
dc.subject.por.fl_str_mv |
Conus magus Cone snail Histopathology Hematology Biochemistry |
topic |
Conus magus Cone snail Histopathology Hematology Biochemistry |
description |
Background:Calcium channel blockers such as conotoxins have shown a great potential to reduce brain and spinal cord injury. MVIIC neuroprotective effects analyzed in in vitromodels of brain and spinal cord ischemia suggest a potential role of this toxin in preventing injury after spinal cord trauma. However, previous clinical studies with MVIIC demonstrated that clinical side effects might limit the usefulness of this drug and there is no research on its systemic effects. Therefore, the present study aimed to investigate the potential toxic effects of MVIIC on organs and to evaluate clinical and blood profiles of rats submitted to spinal cord injury and treated with this marine toxin. Rats were treated with placebo or MVIIC (at doses of 15, 30, 60 or 120 pmol) intralesionally following spinal cord injury. Seven days after the toxin administration, kidney, brain, lung, heart, liver, adrenal, muscles, pancreas, spleen, stomach, and intestine were histopathologically investigated. In addition, blood samples collected from the rats were tested for any hematologic or biochemical changes.Results:The clinical, hematologic and biochemical evaluation revealed no significant abnormalities in all groups, even in high doses. There was no significant alteration in organs, except for degenerative changes in kidneys at a dose of 120 pmol.Conclusions:These findings suggest that MVIIC at 15, 30 and 60 pmol are safe for intralesional administration after spinal cord injury and could be further investigated in relation to its neuroprotective effects. However, 120 pmol doses of MVIIC may provoke adverse effects on kidney tissue. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200311 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200311 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1186/1678-9199-20-15 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP/UNESP) |
dc.source.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases v.20 2014 reponame:The Journal of venomous animals and toxins including tropical diseases (Online) instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
The Journal of venomous animals and toxins including tropical diseases (Online) |
collection |
The Journal of venomous animals and toxins including tropical diseases (Online) |
repository.name.fl_str_mv |
The Journal of venomous animals and toxins including tropical diseases (Online) - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
||editorial@jvat.org.br |
_version_ |
1748958539590664192 |