Effect of Hamamelis virginiana, Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M plant extracts on the phenotypic expression of virulence factors in biofilms of the Candida albicans

Detalhes bibliográficos
Autor(a) principal: Santos, Juliana Guimarães
Data de Publicação: 2024
Outros Autores: Amêndola, Isabela, Lima Bessa, Ellen Roberta, Back-Brito, Grazialla Nuernberg, Oliveira, Luciane Dias
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Science
Texto Completo: https://ojs.ict.unesp.br/index.php/cob/article/view/4039
Resumo: Objective: Analyze the phenotypic expression of virulence factors in Candida albicans biofilms against plant glycolic extracts. Material and Methods: The biofilms of Candida albicans (ATCC 18804) obtained from incubation for 48 hours were exposed for 5 minutes and 24 hours to different concentrations of glycolic extracts of Hamamelis virginiana and Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M, in order to verify the antifungal activity of the proteinase, phospholipase and hemolysin. Results: All extracts were effective in reducing biofilm. In contact for 5 minutes. the extracts reduced 50% of the biofilm. After 24 hours, the Persea americana extract showed the biofilm at 90%, followed by Cynara scolymus, which interrupted it at 85%, There was a change in proteinase intensity after 5 minutes and 24 hours. with an average enzymatic activity of 0.69 compared to the control of 0.49. Cynara scolymus was the extract with the highest mean concentration of 100 mg/ml; the phospholipase intensity was changed with Stryphnodendron barbatiman being more effective in 24 hours compared to the control (p< 0.0001). The hemolysin secretion was modified by Hamamelis virginiana (12.5 mg/ml) after 5 minutes of exposure, and in 24 hours. all extracts were capable to cause changes in secretion. Conclusion: The tested extracts have antifungal potential in Candida albicans biofilms, implying a significant reduction in virulence factors. Thus, these can be indicated as an alternative therapeutic tool to reduce the morbidity of these infections, as in both investigated exposure times. they were able to reduce the enzymatic secretion of the fungus. KEYWORDS Antifungal agents; Candida albicans; Infection; Plant extracts; Virulence factors.
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spelling Effect of Hamamelis virginiana, Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M plant extracts on the phenotypic expression of virulence factors in biofilms of the Candida albicansObjective: Analyze the phenotypic expression of virulence factors in Candida albicans biofilms against plant glycolic extracts. Material and Methods: The biofilms of Candida albicans (ATCC 18804) obtained from incubation for 48 hours were exposed for 5 minutes and 24 hours to different concentrations of glycolic extracts of Hamamelis virginiana and Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M, in order to verify the antifungal activity of the proteinase, phospholipase and hemolysin. Results: All extracts were effective in reducing biofilm. In contact for 5 minutes. the extracts reduced 50% of the biofilm. After 24 hours, the Persea americana extract showed the biofilm at 90%, followed by Cynara scolymus, which interrupted it at 85%, There was a change in proteinase intensity after 5 minutes and 24 hours. with an average enzymatic activity of 0.69 compared to the control of 0.49. Cynara scolymus was the extract with the highest mean concentration of 100 mg/ml; the phospholipase intensity was changed with Stryphnodendron barbatiman being more effective in 24 hours compared to the control (p< 0.0001). The hemolysin secretion was modified by Hamamelis virginiana (12.5 mg/ml) after 5 minutes of exposure, and in 24 hours. all extracts were capable to cause changes in secretion. Conclusion: The tested extracts have antifungal potential in Candida albicans biofilms, implying a significant reduction in virulence factors. Thus, these can be indicated as an alternative therapeutic tool to reduce the morbidity of these infections, as in both investigated exposure times. they were able to reduce the enzymatic secretion of the fungus. KEYWORDS Antifungal agents; Candida albicans; Infection; Plant extracts; Virulence factors.Institute of Science and Technology of São José dos Campos2024-03-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://ojs.ict.unesp.br/index.php/cob/article/view/403910.4322/bds.2024.e4039Brazilian Dental Science; Vol. 27 No. 1 (2024): Jan - Mar / 2024 - Published Jan 2024Brazilian Dental Science; v. 27 n. 1 (2024): Jan - Mar / 2024 - Published Jan 20242178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/4039/4700https://ojs.ict.unesp.br/index.php/cob/article/view/4039/4827Copyright (c) 2024 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessSantos, Juliana GuimarãesAmêndola, IsabelaLima Bessa, Ellen RobertaBack-Brito, Grazialla NuernbergOliveira, Luciane Dias2024-04-01T17:45:19Zoai:ojs.pkp.sfu.ca:article/4039Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br||bds.ict@unesp.br2178-60112178-6011opendoar:2024-04-01T17:45:19Brazilian Dental Science - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effect of Hamamelis virginiana, Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M plant extracts on the phenotypic expression of virulence factors in biofilms of the Candida albicans
title Effect of Hamamelis virginiana, Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M plant extracts on the phenotypic expression of virulence factors in biofilms of the Candida albicans
spellingShingle Effect of Hamamelis virginiana, Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M plant extracts on the phenotypic expression of virulence factors in biofilms of the Candida albicans
Santos, Juliana Guimarães
title_short Effect of Hamamelis virginiana, Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M plant extracts on the phenotypic expression of virulence factors in biofilms of the Candida albicans
title_full Effect of Hamamelis virginiana, Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M plant extracts on the phenotypic expression of virulence factors in biofilms of the Candida albicans
title_fullStr Effect of Hamamelis virginiana, Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M plant extracts on the phenotypic expression of virulence factors in biofilms of the Candida albicans
title_full_unstemmed Effect of Hamamelis virginiana, Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M plant extracts on the phenotypic expression of virulence factors in biofilms of the Candida albicans
title_sort Effect of Hamamelis virginiana, Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M plant extracts on the phenotypic expression of virulence factors in biofilms of the Candida albicans
author Santos, Juliana Guimarães
author_facet Santos, Juliana Guimarães
Amêndola, Isabela
Lima Bessa, Ellen Roberta
Back-Brito, Grazialla Nuernberg
Oliveira, Luciane Dias
author_role author
author2 Amêndola, Isabela
Lima Bessa, Ellen Roberta
Back-Brito, Grazialla Nuernberg
Oliveira, Luciane Dias
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santos, Juliana Guimarães
Amêndola, Isabela
Lima Bessa, Ellen Roberta
Back-Brito, Grazialla Nuernberg
Oliveira, Luciane Dias
description Objective: Analyze the phenotypic expression of virulence factors in Candida albicans biofilms against plant glycolic extracts. Material and Methods: The biofilms of Candida albicans (ATCC 18804) obtained from incubation for 48 hours were exposed for 5 minutes and 24 hours to different concentrations of glycolic extracts of Hamamelis virginiana and Persea americana, Cynara scolymus L and Stryphnodendron barbatiman M, in order to verify the antifungal activity of the proteinase, phospholipase and hemolysin. Results: All extracts were effective in reducing biofilm. In contact for 5 minutes. the extracts reduced 50% of the biofilm. After 24 hours, the Persea americana extract showed the biofilm at 90%, followed by Cynara scolymus, which interrupted it at 85%, There was a change in proteinase intensity after 5 minutes and 24 hours. with an average enzymatic activity of 0.69 compared to the control of 0.49. Cynara scolymus was the extract with the highest mean concentration of 100 mg/ml; the phospholipase intensity was changed with Stryphnodendron barbatiman being more effective in 24 hours compared to the control (p< 0.0001). The hemolysin secretion was modified by Hamamelis virginiana (12.5 mg/ml) after 5 minutes of exposure, and in 24 hours. all extracts were capable to cause changes in secretion. Conclusion: The tested extracts have antifungal potential in Candida albicans biofilms, implying a significant reduction in virulence factors. Thus, these can be indicated as an alternative therapeutic tool to reduce the morbidity of these infections, as in both investigated exposure times. they were able to reduce the enzymatic secretion of the fungus. KEYWORDS Antifungal agents; Candida albicans; Infection; Plant extracts; Virulence factors.
publishDate 2024
dc.date.none.fl_str_mv 2024-03-05
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.ict.unesp.br/index.php/cob/article/view/4039
10.4322/bds.2024.e4039
url https://ojs.ict.unesp.br/index.php/cob/article/view/4039
identifier_str_mv 10.4322/bds.2024.e4039
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.ict.unesp.br/index.php/cob/article/view/4039/4700
https://ojs.ict.unesp.br/index.php/cob/article/view/4039/4827
dc.rights.driver.fl_str_mv Copyright (c) 2024 Brazilian Dental Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2024 Brazilian Dental Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Institute of Science and Technology of São José dos Campos
publisher.none.fl_str_mv Institute of Science and Technology of São José dos Campos
dc.source.none.fl_str_mv Brazilian Dental Science; Vol. 27 No. 1 (2024): Jan - Mar / 2024 - Published Jan 2024
Brazilian Dental Science; v. 27 n. 1 (2024): Jan - Mar / 2024 - Published Jan 2024
2178-6011
reponame:Brazilian Dental Science
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Brazilian Dental Science
collection Brazilian Dental Science
repository.name.fl_str_mv Brazilian Dental Science - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv sergio@fosjc.unesp.br||sergio@fosjc.unesp.br||bds.ict@unesp.br
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