Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices

Detalhes bibliográficos
Autor(a) principal: Fernandes, Ellen Eduarda
Data de Publicação: 2023
Outros Autores: Oliveira, Daniela Fernandes Lobo Molica, Jóias, Renata Pilli, Diniz, Pamela Aparecida, Barros, Patrícia Pimentel de, Jorge, Antonio Olavo Cardoso, Oliveira, Wagner de, Rode, Sigmar de Mello
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Science
Texto Completo: https://ojs.ict.unesp.br/index.php/cob/article/view/3440
Resumo: Objective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms. KEYWORDS Biofilms, Candida albicans, Orthodontic appliances, Orthodontic brackets, Scanning electron microscopy.
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spelling Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devicesObjective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms. KEYWORDS Biofilms, Candida albicans, Orthodontic appliances, Orthodontic brackets, Scanning electron microscopy.Institute of Science and Technology of São José dos Campos2023-04-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.ict.unesp.br/index.php/cob/article/view/344010.4322/bds.2023.e3440Brazilian Dental Science; Vol. 26 No. 2 (2023): Apr - Jun / 2023 - Published Mar 2023Brazilian Dental Science; v. 26 n. 2 (2023): Apr - Jun / 2023 - Published Mar 20232178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/3440/4624Copyright (c) 2023 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessFernandes, Ellen EduardaOliveira, Daniela Fernandes Lobo Molica Jóias, Renata PilliDiniz, Pamela Aparecida Barros, Patrícia Pimentel deJorge, Antonio Olavo Cardoso Oliveira, Wagner de Rode, Sigmar de Mello2023-06-13T17:03:30Zoai:ojs.pkp.sfu.ca:article/3440Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2023-06-13T17:03:30Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)false
dc.title.none.fl_str_mv Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
title Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
spellingShingle Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
Fernandes, Ellen Eduarda
title_short Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
title_full Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
title_fullStr Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
title_full_unstemmed Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
title_sort Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
author Fernandes, Ellen Eduarda
author_facet Fernandes, Ellen Eduarda
Oliveira, Daniela Fernandes Lobo Molica
Jóias, Renata Pilli
Diniz, Pamela Aparecida
Barros, Patrícia Pimentel de
Jorge, Antonio Olavo Cardoso
Oliveira, Wagner de
Rode, Sigmar de Mello
author_role author
author2 Oliveira, Daniela Fernandes Lobo Molica
Jóias, Renata Pilli
Diniz, Pamela Aparecida
Barros, Patrícia Pimentel de
Jorge, Antonio Olavo Cardoso
Oliveira, Wagner de
Rode, Sigmar de Mello
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fernandes, Ellen Eduarda
Oliveira, Daniela Fernandes Lobo Molica
Jóias, Renata Pilli
Diniz, Pamela Aparecida
Barros, Patrícia Pimentel de
Jorge, Antonio Olavo Cardoso
Oliveira, Wagner de
Rode, Sigmar de Mello
description Objective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms. KEYWORDS Biofilms, Candida albicans, Orthodontic appliances, Orthodontic brackets, Scanning electron microscopy.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-18
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.ict.unesp.br/index.php/cob/article/view/3440
10.4322/bds.2023.e3440
url https://ojs.ict.unesp.br/index.php/cob/article/view/3440
identifier_str_mv 10.4322/bds.2023.e3440
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.ict.unesp.br/index.php/cob/article/view/3440/4624
dc.rights.driver.fl_str_mv Copyright (c) 2023 Brazilian Dental Science
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Brazilian Dental Science
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Institute of Science and Technology of São José dos Campos
publisher.none.fl_str_mv Institute of Science and Technology of São José dos Campos
dc.source.none.fl_str_mv Brazilian Dental Science; Vol. 26 No. 2 (2023): Apr - Jun / 2023 - Published Mar 2023
Brazilian Dental Science; v. 26 n. 2 (2023): Apr - Jun / 2023 - Published Mar 2023
2178-6011
reponame:Brazilian Dental Science
instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Brazilian Dental Science
collection Brazilian Dental Science
repository.name.fl_str_mv Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
repository.mail.fl_str_mv sergio@fosjc.unesp.br||sergio@fosjc.unesp.br
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