Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Science |
Texto Completo: | https://ojs.ict.unesp.br/index.php/cob/article/view/3440 |
Resumo: | Objective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms. KEYWORDS Biofilms, Candida albicans, Orthodontic appliances, Orthodontic brackets, Scanning electron microscopy. |
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UNESP-20_2e47961961489f2a297fea27385701d3 |
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oai:ojs.pkp.sfu.ca:article/3440 |
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UNESP-20 |
network_name_str |
Brazilian Dental Science |
repository_id_str |
|
spelling |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devicesObjective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms. KEYWORDS Biofilms, Candida albicans, Orthodontic appliances, Orthodontic brackets, Scanning electron microscopy.Institute of Science and Technology of São José dos Campos2023-04-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.ict.unesp.br/index.php/cob/article/view/344010.4322/bds.2023.e3440Brazilian Dental Science; Vol. 26 No. 2 (2023): Apr - Jun / 2023 - Published Mar 2023Brazilian Dental Science; v. 26 n. 2 (2023): Apr - Jun / 2023 - Published Mar 20232178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/3440/4624Copyright (c) 2023 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessFernandes, Ellen EduardaOliveira, Daniela Fernandes Lobo Molica Jóias, Renata PilliDiniz, Pamela Aparecida Barros, Patrícia Pimentel deJorge, Antonio Olavo Cardoso Oliveira, Wagner de Rode, Sigmar de Mello2023-06-13T17:03:30Zoai:ojs.pkp.sfu.ca:article/3440Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2023-06-13T17:03:30Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
title |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
spellingShingle |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices Fernandes, Ellen Eduarda |
title_short |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
title_full |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
title_fullStr |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
title_full_unstemmed |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
title_sort |
Analysis of biofilm formation by Candida albicans in different types of orthodontic fixed appliances and devices |
author |
Fernandes, Ellen Eduarda |
author_facet |
Fernandes, Ellen Eduarda Oliveira, Daniela Fernandes Lobo Molica Jóias, Renata Pilli Diniz, Pamela Aparecida Barros, Patrícia Pimentel de Jorge, Antonio Olavo Cardoso Oliveira, Wagner de Rode, Sigmar de Mello |
author_role |
author |
author2 |
Oliveira, Daniela Fernandes Lobo Molica Jóias, Renata Pilli Diniz, Pamela Aparecida Barros, Patrícia Pimentel de Jorge, Antonio Olavo Cardoso Oliveira, Wagner de Rode, Sigmar de Mello |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, Ellen Eduarda Oliveira, Daniela Fernandes Lobo Molica Jóias, Renata Pilli Diniz, Pamela Aparecida Barros, Patrícia Pimentel de Jorge, Antonio Olavo Cardoso Oliveira, Wagner de Rode, Sigmar de Mello |
description |
Objective: in this study, biofilm formation by Candida albicans in fixed orthodontic appliances was evaluated. Material and Methods: a total of 300 conventional metal brackets (MC), ceramic (CB), self-ligation (SLB), nickel-titanium (NiTi), and nickel-chromium (NiCr) wires, and ligatures types were organized into thirty groups (n=10). To induce biofilm formation, brackets, wires, and ligatures were joined, sterilized, placed in 24-well plates, contaminated with standardized suspensions of C. albicans (107 cells/mL), and incubated at 37 °C for 48 h with shaking. The biofilms formed were detached using an ultrasonic homogenizer, and suspensions were serially diluted and plated on Sabouraud dextrose agar to determine colony-forming units per mL. Scanning electron microscopy was performed before and after the biofilm formation. Results: lower amount of biofilm formation was observed in the MC group than in the CB and SLB groups (p<0.0001). SLB and CB showed similar biofilm formation rates (p=0.855). In general, the cross-sectional wires .018”x.025” showed higher biofilm formation when associated with the three types of brackets. When brackets, wires, and ligatures were associated, the sets with NiCr wires and SSL ligatures with MC brackets (p=0.0008) and CB (p=0.0003) showed higher biofilm formation. Conclusion: thus, brackets of MC with NiTi and NiCr wires showed lower biofilm formation, regardless of the ligature and cross-sectional or gauge of the wire and, MC and CB brackets with NiCr wires and SSL ligatures were more likely to accumulate biofilms. KEYWORDS Biofilms, Candida albicans, Orthodontic appliances, Orthodontic brackets, Scanning electron microscopy. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-18 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/3440 10.4322/bds.2023.e3440 |
url |
https://ojs.ict.unesp.br/index.php/cob/article/view/3440 |
identifier_str_mv |
10.4322/bds.2023.e3440 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/3440/4624 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Brazilian Dental Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Brazilian Dental Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
dc.source.none.fl_str_mv |
Brazilian Dental Science; Vol. 26 No. 2 (2023): Apr - Jun / 2023 - Published Mar 2023 Brazilian Dental Science; v. 26 n. 2 (2023): Apr - Jun / 2023 - Published Mar 2023 2178-6011 reponame:Brazilian Dental Science instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Brazilian Dental Science |
collection |
Brazilian Dental Science |
repository.name.fl_str_mv |
Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
sergio@fosjc.unesp.br||sergio@fosjc.unesp.br |
_version_ |
1800214394610647040 |