Schinus terebinthifolius (Brazilian Peppertree) extract used as antifungal to control Candida spp. in planktonic cultures and biofilms
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Science |
Texto Completo: | https://ojs.ict.unesp.br/index.php/cob/article/view/2077 |
Resumo: | Objective: The use of medicinal plants may be an alternative method for the control of Candida spp. responsible for human infections. This study evaluated the antifungal effect of Schinus terebinthifolius extract (Brazilian Peppertree) on C. albicans, C. dubliniensis, C. glabrata, and C. krusei planktonic cultures and biofilms. Material and Methods: Minimum inhibitory concentration (MIC) and minimum fungal concentration (MFC) of the plant extract were determined by the broth microdilution method. Biofilms formed in microplate wells were exposed to the extract for 5 min (50, 100 and 200 mg/mL) or 24 h (25, 50 and 100 mg/mL). After determination of colony-forming units per milliliter (CFU/mL), the data were analyzed by one-way ANOVA and Tukey’s Test (P ? 0.05). Results: Different MIC (mg/mL) were found, such as 0.39 (C. dubliniensis), 1.56 (C. albicans), and 3.13 (C. glabrata and C. krusei). Besides, MFC (mg/mL) of 0.78 (C. dubliniensis) and 3.13 (C. albicans, C. glabrata and C. krusei) were also observed. Regarding the biofilms, significant reductions (log10) were found after 5 min and 24 h exposure to the plant extract, compared to the control group. However, C. dubliniensis was significantly affected only in 24 h treatment. Conclusion: S. terebinthifolius extract presented a significant antifungal effect on C. albicans, C. dubliniensis, C. glabrata, and C. Krusei both in planktonic cultures and biofilms. |
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oai:ojs.pkp.sfu.ca:article/2077 |
network_acronym_str |
UNESP-20 |
network_name_str |
Brazilian Dental Science |
repository_id_str |
|
spelling |
Schinus terebinthifolius (Brazilian Peppertree) extract used as antifungal to control Candida spp. in planktonic cultures and biofilmsObjective: The use of medicinal plants may be an alternative method for the control of Candida spp. responsible for human infections. This study evaluated the antifungal effect of Schinus terebinthifolius extract (Brazilian Peppertree) on C. albicans, C. dubliniensis, C. glabrata, and C. krusei planktonic cultures and biofilms. Material and Methods: Minimum inhibitory concentration (MIC) and minimum fungal concentration (MFC) of the plant extract were determined by the broth microdilution method. Biofilms formed in microplate wells were exposed to the extract for 5 min (50, 100 and 200 mg/mL) or 24 h (25, 50 and 100 mg/mL). After determination of colony-forming units per milliliter (CFU/mL), the data were analyzed by one-way ANOVA and Tukey’s Test (P ? 0.05). Results: Different MIC (mg/mL) were found, such as 0.39 (C. dubliniensis), 1.56 (C. albicans), and 3.13 (C. glabrata and C. krusei). Besides, MFC (mg/mL) of 0.78 (C. dubliniensis) and 3.13 (C. albicans, C. glabrata and C. krusei) were also observed. Regarding the biofilms, significant reductions (log10) were found after 5 min and 24 h exposure to the plant extract, compared to the control group. However, C. dubliniensis was significantly affected only in 24 h treatment. Conclusion: S. terebinthifolius extract presented a significant antifungal effect on C. albicans, C. dubliniensis, C. glabrata, and C. Krusei both in planktonic cultures and biofilms.Institute of Science and Technology of São José dos Campos2020-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.ict.unesp.br/index.php/cob/article/view/207710.14295/bds.2020.v23i4.2077Brazilian Dental Science; Vol. 23 No. 4 (2020): Oct - Dec / 2020 - published Sep 2020; 7pBrazilian Dental Science; v. 23 n. 4 (2020): Oct - Dec / 2020 - published Sep 2020; 7p2178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/2077/4176Copyright (c) 2020 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessViegas, Daiane de JesusAmêndola, IsabelaBotrel, Tássia Marchettide Oliveira, Felipe EduardoFigueira, Leandro WagnerSper, Fábia Luglide Oliveira, Jonatas Rafaelde Oliveira, Luciane Dias2020-09-30T23:03:30Zoai:ojs.pkp.sfu.ca:article/2077Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:30:31.039114Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true |
dc.title.none.fl_str_mv |
Schinus terebinthifolius (Brazilian Peppertree) extract used as antifungal to control Candida spp. in planktonic cultures and biofilms |
title |
Schinus terebinthifolius (Brazilian Peppertree) extract used as antifungal to control Candida spp. in planktonic cultures and biofilms |
spellingShingle |
Schinus terebinthifolius (Brazilian Peppertree) extract used as antifungal to control Candida spp. in planktonic cultures and biofilms Viegas, Daiane de Jesus |
title_short |
Schinus terebinthifolius (Brazilian Peppertree) extract used as antifungal to control Candida spp. in planktonic cultures and biofilms |
title_full |
Schinus terebinthifolius (Brazilian Peppertree) extract used as antifungal to control Candida spp. in planktonic cultures and biofilms |
title_fullStr |
Schinus terebinthifolius (Brazilian Peppertree) extract used as antifungal to control Candida spp. in planktonic cultures and biofilms |
title_full_unstemmed |
Schinus terebinthifolius (Brazilian Peppertree) extract used as antifungal to control Candida spp. in planktonic cultures and biofilms |
title_sort |
Schinus terebinthifolius (Brazilian Peppertree) extract used as antifungal to control Candida spp. in planktonic cultures and biofilms |
author |
Viegas, Daiane de Jesus |
author_facet |
Viegas, Daiane de Jesus Amêndola, Isabela Botrel, Tássia Marchetti de Oliveira, Felipe Eduardo Figueira, Leandro Wagner Sper, Fábia Lugli de Oliveira, Jonatas Rafael de Oliveira, Luciane Dias |
author_role |
author |
author2 |
Amêndola, Isabela Botrel, Tássia Marchetti de Oliveira, Felipe Eduardo Figueira, Leandro Wagner Sper, Fábia Lugli de Oliveira, Jonatas Rafael de Oliveira, Luciane Dias |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Viegas, Daiane de Jesus Amêndola, Isabela Botrel, Tássia Marchetti de Oliveira, Felipe Eduardo Figueira, Leandro Wagner Sper, Fábia Lugli de Oliveira, Jonatas Rafael de Oliveira, Luciane Dias |
description |
Objective: The use of medicinal plants may be an alternative method for the control of Candida spp. responsible for human infections. This study evaluated the antifungal effect of Schinus terebinthifolius extract (Brazilian Peppertree) on C. albicans, C. dubliniensis, C. glabrata, and C. krusei planktonic cultures and biofilms. Material and Methods: Minimum inhibitory concentration (MIC) and minimum fungal concentration (MFC) of the plant extract were determined by the broth microdilution method. Biofilms formed in microplate wells were exposed to the extract for 5 min (50, 100 and 200 mg/mL) or 24 h (25, 50 and 100 mg/mL). After determination of colony-forming units per milliliter (CFU/mL), the data were analyzed by one-way ANOVA and Tukey’s Test (P ? 0.05). Results: Different MIC (mg/mL) were found, such as 0.39 (C. dubliniensis), 1.56 (C. albicans), and 3.13 (C. glabrata and C. krusei). Besides, MFC (mg/mL) of 0.78 (C. dubliniensis) and 3.13 (C. albicans, C. glabrata and C. krusei) were also observed. Regarding the biofilms, significant reductions (log10) were found after 5 min and 24 h exposure to the plant extract, compared to the control group. However, C. dubliniensis was significantly affected only in 24 h treatment. Conclusion: S. terebinthifolius extract presented a significant antifungal effect on C. albicans, C. dubliniensis, C. glabrata, and C. Krusei both in planktonic cultures and biofilms. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/2077 10.14295/bds.2020.v23i4.2077 |
url |
https://ojs.ict.unesp.br/index.php/cob/article/view/2077 |
identifier_str_mv |
10.14295/bds.2020.v23i4.2077 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/2077/4176 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Brazilian Dental Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Brazilian Dental Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
dc.source.none.fl_str_mv |
Brazilian Dental Science; Vol. 23 No. 4 (2020): Oct - Dec / 2020 - published Sep 2020; 7p Brazilian Dental Science; v. 23 n. 4 (2020): Oct - Dec / 2020 - published Sep 2020; 7p 2178-6011 reponame:Brazilian Dental Science instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Brazilian Dental Science |
collection |
Brazilian Dental Science |
repository.name.fl_str_mv |
Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
sergio@fosjc.unesp.br||sergio@fosjc.unesp.br |
_version_ |
1788346901751398400 |