Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic Therapy
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Science |
Texto Completo: | https://ojs.ict.unesp.br/index.php/cob/article/view/1753 |
Resumo: | Objective: The aim of this study was to evaluate the impact on the isolation and characterization of stem cells from pulp tissues obtained through rotary instrumentation techniques compared to the manual technique. Material and Methods: Thirty permanent teeth were included, 15 of which were instrumented with rotational technique (Protaper SX) and other 15 with manual technique. Cells obtained were characterized by flow cytometry and proliferation was evaluated by the MTT assay. The plasticity was evaluated for adipogenic, osteogenic and odontogenic differentiations. Results: Cells isolated from the pulp of permanent teeth, by manual techniques, presented fibroblast morphology and were able to differentiate successfully. All lineages expressed CD29, CD73, CD90, CD105, CD146, CD166 and were negative for CD31, CD34 and CD45. MTT assay showing significantly increased proliferation of hDPSCs in 5 and 7 days of the culture. Conclusion: The present study demonstrated that manual instrumentation technique is one of the best candidates to harvest dental pulp tissue as the dental stem cell source due to ability effective expanded with less tissue invasion. The technique of rotational instrumentation proved to be very harmful to the tissues of the dental pulp, and we can’t obtain cells using this technique.Keywords: Root Canal Therapy; Pulpectomy; Anatomy and Histology; Stem Cell. |
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Brazilian Dental Science |
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Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic TherapyObjective: The aim of this study was to evaluate the impact on the isolation and characterization of stem cells from pulp tissues obtained through rotary instrumentation techniques compared to the manual technique. Material and Methods: Thirty permanent teeth were included, 15 of which were instrumented with rotational technique (Protaper SX) and other 15 with manual technique. Cells obtained were characterized by flow cytometry and proliferation was evaluated by the MTT assay. The plasticity was evaluated for adipogenic, osteogenic and odontogenic differentiations. Results: Cells isolated from the pulp of permanent teeth, by manual techniques, presented fibroblast morphology and were able to differentiate successfully. All lineages expressed CD29, CD73, CD90, CD105, CD146, CD166 and were negative for CD31, CD34 and CD45. MTT assay showing significantly increased proliferation of hDPSCs in 5 and 7 days of the culture. Conclusion: The present study demonstrated that manual instrumentation technique is one of the best candidates to harvest dental pulp tissue as the dental stem cell source due to ability effective expanded with less tissue invasion. The technique of rotational instrumentation proved to be very harmful to the tissues of the dental pulp, and we can’t obtain cells using this technique.Keywords: Root Canal Therapy; Pulpectomy; Anatomy and Histology; Stem Cell.Institute of Science and Technology of São José dos Campos2020-01-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentimage/jpegimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffimage/tiffhttps://ojs.ict.unesp.br/index.php/cob/article/view/175310.14295/bds.2020.v23i1.1753Brazilian Dental Science; Vol. 23 No. 1 (2020): Jan. - Mar. - Published Jan. 2020; 10 p.Brazilian Dental Science; v. 23 n. 1 (2020): Jan. - Mar. - Published Jan. 2020; 10 p.2178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/1753/1428https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3676https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3677https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3678https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3679https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3680https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3681https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3682https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3683https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3684Copyright (c) 2020 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessWeiss, Jairo BarrosGonçalves, Fernanda da SilvaMaranduba, Carlos Magno da Costade Resende, Leandro Marquesdo Carmo, Antonio Marcio Resende2020-02-12T15:54:34Zoai:ojs.pkp.sfu.ca:article/1753Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:30:22.647550Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true |
dc.title.none.fl_str_mv |
Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic Therapy |
title |
Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic Therapy |
spellingShingle |
Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic Therapy Weiss, Jairo Barros |
title_short |
Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic Therapy |
title_full |
Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic Therapy |
title_fullStr |
Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic Therapy |
title_full_unstemmed |
Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic Therapy |
title_sort |
Isolation and Characterization of Stem Cells Derived by Human Dental Pulp from Harvest Based in Rotary and Manual Techniques used in Endodontic Therapy |
author |
Weiss, Jairo Barros |
author_facet |
Weiss, Jairo Barros Gonçalves, Fernanda da Silva Maranduba, Carlos Magno da Costa de Resende, Leandro Marques do Carmo, Antonio Marcio Resende |
author_role |
author |
author2 |
Gonçalves, Fernanda da Silva Maranduba, Carlos Magno da Costa de Resende, Leandro Marques do Carmo, Antonio Marcio Resende |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Weiss, Jairo Barros Gonçalves, Fernanda da Silva Maranduba, Carlos Magno da Costa de Resende, Leandro Marques do Carmo, Antonio Marcio Resende |
description |
Objective: The aim of this study was to evaluate the impact on the isolation and characterization of stem cells from pulp tissues obtained through rotary instrumentation techniques compared to the manual technique. Material and Methods: Thirty permanent teeth were included, 15 of which were instrumented with rotational technique (Protaper SX) and other 15 with manual technique. Cells obtained were characterized by flow cytometry and proliferation was evaluated by the MTT assay. The plasticity was evaluated for adipogenic, osteogenic and odontogenic differentiations. Results: Cells isolated from the pulp of permanent teeth, by manual techniques, presented fibroblast morphology and were able to differentiate successfully. All lineages expressed CD29, CD73, CD90, CD105, CD146, CD166 and were negative for CD31, CD34 and CD45. MTT assay showing significantly increased proliferation of hDPSCs in 5 and 7 days of the culture. Conclusion: The present study demonstrated that manual instrumentation technique is one of the best candidates to harvest dental pulp tissue as the dental stem cell source due to ability effective expanded with less tissue invasion. The technique of rotational instrumentation proved to be very harmful to the tissues of the dental pulp, and we can’t obtain cells using this technique.Keywords: Root Canal Therapy; Pulpectomy; Anatomy and Histology; Stem Cell. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1753 10.14295/bds.2020.v23i1.1753 |
url |
https://ojs.ict.unesp.br/index.php/cob/article/view/1753 |
identifier_str_mv |
10.14295/bds.2020.v23i1.1753 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1753/1428 https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3676 https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3677 https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3678 https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3679 https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3680 https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3681 https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3682 https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3683 https://ojs.ict.unesp.br/index.php/cob/article/view/1753/3684 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Brazilian Dental Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Brazilian Dental Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document image/jpeg image/tiff image/tiff image/tiff image/tiff image/tiff image/tiff image/tiff |
dc.publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
dc.source.none.fl_str_mv |
Brazilian Dental Science; Vol. 23 No. 1 (2020): Jan. - Mar. - Published Jan. 2020; 10 p. Brazilian Dental Science; v. 23 n. 1 (2020): Jan. - Mar. - Published Jan. 2020; 10 p. 2178-6011 reponame:Brazilian Dental Science instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Brazilian Dental Science |
collection |
Brazilian Dental Science |
repository.name.fl_str_mv |
Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
sergio@fosjc.unesp.br||sergio@fosjc.unesp.br |
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1788346900831797248 |