Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic rats
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Brazilian Dental Science |
Texto Completo: | https://ojs.ict.unesp.br/index.php/cob/article/view/1089 |
Resumo: | Objective: This study aimed to quantify through immunohistochemistry the expression of bone formation marker osteocalcin during the repair process of autogenous onlay bone graft, associated or not to the resorbable collagen membrane and to compare these findings with the presence of Diabetes Mellitus. Material and Methods: Sixty rats (Rattus norvegicus, albinus variation, Wistar) aged 90 days, were divided into two groups with 30 animals: test group – rats with induced diabetes; control group - normoglycemic rats. All rats received grafts on the left and right hemi-mandible with or without collagen membrane coverage, respectively. The animals were euthanized at the following periods: 0h, 7, 14, 21, 45, and 60 days. Immunohistochemistry analysis was performed by the marker osteocalcin at receptor site-graft interface. To analyze osteocalcin immunohistochemical expression, a panoramic view photograph of the graft was taken followed by two photographs at larger magnification. Results: No statistically significances at 5% level were observed between diabetic and control group with and without membrane; and diabetic and control groups with membrane coverage. Conclusion: Within the limits of this present study, it can be concluded that the osteocalcin marker might be influenced by diabetes so that it was late expressed during this condition. However, the association of the graft with the membrane could improve this delay by reaching expression values similar to those of control group. |
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oai:ojs.pkp.sfu.ca:article/1089 |
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UNESP-20 |
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Brazilian Dental Science |
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spelling |
Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic ratsObjective: This study aimed to quantify through immunohistochemistry the expression of bone formation marker osteocalcin during the repair process of autogenous onlay bone graft, associated or not to the resorbable collagen membrane and to compare these findings with the presence of Diabetes Mellitus. Material and Methods: Sixty rats (Rattus norvegicus, albinus variation, Wistar) aged 90 days, were divided into two groups with 30 animals: test group – rats with induced diabetes; control group - normoglycemic rats. All rats received grafts on the left and right hemi-mandible with or without collagen membrane coverage, respectively. The animals were euthanized at the following periods: 0h, 7, 14, 21, 45, and 60 days. Immunohistochemistry analysis was performed by the marker osteocalcin at receptor site-graft interface. To analyze osteocalcin immunohistochemical expression, a panoramic view photograph of the graft was taken followed by two photographs at larger magnification. Results: No statistically significances at 5% level were observed between diabetic and control group with and without membrane; and diabetic and control groups with membrane coverage. Conclusion: Within the limits of this present study, it can be concluded that the osteocalcin marker might be influenced by diabetes so that it was late expressed during this condition. However, the association of the graft with the membrane could improve this delay by reaching expression values similar to those of control group.Institute of Science and Technology of São José dos Campos2015-05-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfapplication/mswordapplication/mswordhttps://ojs.ict.unesp.br/index.php/cob/article/view/108910.14295/bds.2015.v18i2.1089Brazilian Dental Science; Vol. 18 No. 2 (2015): Apr. - Jun. / 2015 - Published May 2015; 73-81Brazilian Dental Science; v. 18 n. 2 (2015): Apr. - Jun. / 2015 - Published May 2015; 73-812178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPengporhttps://ojs.ict.unesp.br/index.php/cob/article/view/1089/943https://ojs.ict.unesp.br/index.php/cob/article/view/1089/2247https://ojs.ict.unesp.br/index.php/cob/article/view/1089/2248https://ojs.ict.unesp.br/index.php/cob/article/view/1089/2249Bernardo, Daniella VicensottoTera, Tabata de Mellode Marco, Andrea CarvalhoMelo Filho, Antonio Braulino deSantamaria, Mauro PedrineJardini, Maria Aparecida Nevesinfo:eu-repo/semantics/openAccess2020-01-28T12:27:41Zoai:ojs.pkp.sfu.ca:article/1089Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:30:00.302651Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true |
dc.title.none.fl_str_mv |
Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic rats |
title |
Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic rats |
spellingShingle |
Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic rats Bernardo, Daniella Vicensotto |
title_short |
Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic rats |
title_full |
Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic rats |
title_fullStr |
Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic rats |
title_full_unstemmed |
Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic rats |
title_sort |
Osteoclacin expression during autogenous onlay bone grafts with or without resorbable collagen membrane in diabetic rats |
author |
Bernardo, Daniella Vicensotto |
author_facet |
Bernardo, Daniella Vicensotto Tera, Tabata de Mello de Marco, Andrea Carvalho Melo Filho, Antonio Braulino de Santamaria, Mauro Pedrine Jardini, Maria Aparecida Neves |
author_role |
author |
author2 |
Tera, Tabata de Mello de Marco, Andrea Carvalho Melo Filho, Antonio Braulino de Santamaria, Mauro Pedrine Jardini, Maria Aparecida Neves |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Bernardo, Daniella Vicensotto Tera, Tabata de Mello de Marco, Andrea Carvalho Melo Filho, Antonio Braulino de Santamaria, Mauro Pedrine Jardini, Maria Aparecida Neves |
description |
Objective: This study aimed to quantify through immunohistochemistry the expression of bone formation marker osteocalcin during the repair process of autogenous onlay bone graft, associated or not to the resorbable collagen membrane and to compare these findings with the presence of Diabetes Mellitus. Material and Methods: Sixty rats (Rattus norvegicus, albinus variation, Wistar) aged 90 days, were divided into two groups with 30 animals: test group – rats with induced diabetes; control group - normoglycemic rats. All rats received grafts on the left and right hemi-mandible with or without collagen membrane coverage, respectively. The animals were euthanized at the following periods: 0h, 7, 14, 21, 45, and 60 days. Immunohistochemistry analysis was performed by the marker osteocalcin at receptor site-graft interface. To analyze osteocalcin immunohistochemical expression, a panoramic view photograph of the graft was taken followed by two photographs at larger magnification. Results: No statistically significances at 5% level were observed between diabetic and control group with and without membrane; and diabetic and control groups with membrane coverage. Conclusion: Within the limits of this present study, it can be concluded that the osteocalcin marker might be influenced by diabetes so that it was late expressed during this condition. However, the association of the graft with the membrane could improve this delay by reaching expression values similar to those of control group. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1089 10.14295/bds.2015.v18i2.1089 |
url |
https://ojs.ict.unesp.br/index.php/cob/article/view/1089 |
identifier_str_mv |
10.14295/bds.2015.v18i2.1089 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1089/943 https://ojs.ict.unesp.br/index.php/cob/article/view/1089/2247 https://ojs.ict.unesp.br/index.php/cob/article/view/1089/2248 https://ojs.ict.unesp.br/index.php/cob/article/view/1089/2249 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/msword application/msword |
dc.publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
dc.source.none.fl_str_mv |
Brazilian Dental Science; Vol. 18 No. 2 (2015): Apr. - Jun. / 2015 - Published May 2015; 73-81 Brazilian Dental Science; v. 18 n. 2 (2015): Apr. - Jun. / 2015 - Published May 2015; 73-81 2178-6011 reponame:Brazilian Dental Science instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Brazilian Dental Science |
collection |
Brazilian Dental Science |
repository.name.fl_str_mv |
Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
sergio@fosjc.unesp.br||sergio@fosjc.unesp.br |
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1788346899298779136 |