Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Dental Science |
Texto Completo: | https://ojs.ict.unesp.br/index.php/cob/article/view/1317 |
Resumo: | Objective: This study evaluated the effect of rosemary extract on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa monomicrobial biofilms viability, as well as on C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms. Material and Methods: In microtiter plate, mono- and polymicrobial biofilms for 48 h were formed. Then, they were exposed for 5 min to rosemary extract (200 mg/mL). Saline (0.9% NaCl) was used as control. After, washes were done with saline to remove the affected cells. Biofilm viability was checked by MTT colorimetric assay, after treatment. Absorbance of the wells was read in microplate spectrophotometer (570 nm) and data were converted to reduction percentage and statistically analyzed by ANOVA and Tukey test (p ? 0.05). Results: After application of rosemary extract, with exception of the E. faecalis biofilm, significant reductions in mono- and polymicrobial biofilms viability were observed. Conclusion: C. albicans, S. aureus, S. mutans and P. aeruginosa monomicrobial biofilms were affected by rosemary extract, as well as C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms, presenting significant viability reductions. |
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UNESP-20_fbe7bc627f1debd902715aa383b50fca |
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oai:ojs.pkp.sfu.ca:article/1317 |
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UNESP-20 |
network_name_str |
Brazilian Dental Science |
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spelling |
Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interestObjective: This study evaluated the effect of rosemary extract on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa monomicrobial biofilms viability, as well as on C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms. Material and Methods: In microtiter plate, mono- and polymicrobial biofilms for 48 h were formed. Then, they were exposed for 5 min to rosemary extract (200 mg/mL). Saline (0.9% NaCl) was used as control. After, washes were done with saline to remove the affected cells. Biofilm viability was checked by MTT colorimetric assay, after treatment. Absorbance of the wells was read in microplate spectrophotometer (570 nm) and data were converted to reduction percentage and statistically analyzed by ANOVA and Tukey test (p ? 0.05). Results: After application of rosemary extract, with exception of the E. faecalis biofilm, significant reductions in mono- and polymicrobial biofilms viability were observed. Conclusion: C. albicans, S. aureus, S. mutans and P. aeruginosa monomicrobial biofilms were affected by rosemary extract, as well as C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms, presenting significant viability reductions. Institute of Science and Technology of São José dos Campos2017-02-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfimage/jpegimage/jpegimage/jpegimage/jpegimage/jpeghttps://ojs.ict.unesp.br/index.php/cob/article/view/131710.14295/bds.2017.v20i1.1317Brazilian Dental Science; Vol. 20 No. 1 (2017): Jan. - Mar. / 2017 - Published February 2017; 64-69Brazilian Dental Science; v. 20 n. 1 (2017): Jan. - Mar. / 2017 - Published February 2017; 64-692178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/1317/1103https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2811https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2812https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2813https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2814https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2815https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2816https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2817Copyright (c) 2017 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessde Oliveira, Jonatas RafaeJesus, Daiane deOliveira, Luciane Dias de2020-01-28T12:15:37Zoai:ojs.pkp.sfu.ca:article/1317Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:30:09.277620Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true |
dc.title.none.fl_str_mv |
Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest |
title |
Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest |
spellingShingle |
Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest de Oliveira, Jonatas Rafae |
title_short |
Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest |
title_full |
Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest |
title_fullStr |
Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest |
title_full_unstemmed |
Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest |
title_sort |
Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest |
author |
de Oliveira, Jonatas Rafae |
author_facet |
de Oliveira, Jonatas Rafae Jesus, Daiane de Oliveira, Luciane Dias de |
author_role |
author |
author2 |
Jesus, Daiane de Oliveira, Luciane Dias de |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
de Oliveira, Jonatas Rafae Jesus, Daiane de Oliveira, Luciane Dias de |
description |
Objective: This study evaluated the effect of rosemary extract on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa monomicrobial biofilms viability, as well as on C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms. Material and Methods: In microtiter plate, mono- and polymicrobial biofilms for 48 h were formed. Then, they were exposed for 5 min to rosemary extract (200 mg/mL). Saline (0.9% NaCl) was used as control. After, washes were done with saline to remove the affected cells. Biofilm viability was checked by MTT colorimetric assay, after treatment. Absorbance of the wells was read in microplate spectrophotometer (570 nm) and data were converted to reduction percentage and statistically analyzed by ANOVA and Tukey test (p ? 0.05). Results: After application of rosemary extract, with exception of the E. faecalis biofilm, significant reductions in mono- and polymicrobial biofilms viability were observed. Conclusion: C. albicans, S. aureus, S. mutans and P. aeruginosa monomicrobial biofilms were affected by rosemary extract, as well as C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms, presenting significant viability reductions. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-02-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1317 10.14295/bds.2017.v20i1.1317 |
url |
https://ojs.ict.unesp.br/index.php/cob/article/view/1317 |
identifier_str_mv |
10.14295/bds.2017.v20i1.1317 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.ict.unesp.br/index.php/cob/article/view/1317/1103 https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2811 https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2812 https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2813 https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2814 https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2815 https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2816 https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2817 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Brazilian Dental Science info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Brazilian Dental Science |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document application/pdf image/jpeg image/jpeg image/jpeg image/jpeg image/jpeg |
dc.publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
publisher.none.fl_str_mv |
Institute of Science and Technology of São José dos Campos |
dc.source.none.fl_str_mv |
Brazilian Dental Science; Vol. 20 No. 1 (2017): Jan. - Mar. / 2017 - Published February 2017; 64-69 Brazilian Dental Science; v. 20 n. 1 (2017): Jan. - Mar. / 2017 - Published February 2017; 64-69 2178-6011 reponame:Brazilian Dental Science instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Brazilian Dental Science |
collection |
Brazilian Dental Science |
repository.name.fl_str_mv |
Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
sergio@fosjc.unesp.br||sergio@fosjc.unesp.br |
_version_ |
1788346899785318400 |