Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest

Detalhes bibliográficos
Autor(a) principal: de Oliveira, Jonatas Rafae
Data de Publicação: 2017
Outros Autores: Jesus, Daiane de, Oliveira, Luciane Dias de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Dental Science
Texto Completo: https://ojs.ict.unesp.br/index.php/cob/article/view/1317
Resumo: Objective: This study evaluated the effect of rosemary extract on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa monomicrobial biofilms viability, as well as on C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms. Material and Methods: In microtiter plate, mono- and polymicrobial biofilms for 48 h were formed. Then, they were exposed for 5 min to rosemary extract (200 mg/mL). Saline (0.9% NaCl) was used as control. After, washes were done with saline to remove the affected cells. Biofilm viability was checked by MTT colorimetric assay, after treatment. Absorbance of the wells was read in microplate spectrophotometer (570 nm) and data were converted to reduction percentage and statistically analyzed by ANOVA and Tukey test (p ? 0.05). Results: After application of rosemary extract, with exception of the E. faecalis biofilm, significant reductions in mono- and polymicrobial biofilms viability were observed. Conclusion: C. albicans, S. aureus, S. mutans and P. aeruginosa monomicrobial biofilms were affected by rosemary extract, as well as C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms, presenting significant viability reductions.   
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spelling Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interestObjective: This study evaluated the effect of rosemary extract on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa monomicrobial biofilms viability, as well as on C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms. Material and Methods: In microtiter plate, mono- and polymicrobial biofilms for 48 h were formed. Then, they were exposed for 5 min to rosemary extract (200 mg/mL). Saline (0.9% NaCl) was used as control. After, washes were done with saline to remove the affected cells. Biofilm viability was checked by MTT colorimetric assay, after treatment. Absorbance of the wells was read in microplate spectrophotometer (570 nm) and data were converted to reduction percentage and statistically analyzed by ANOVA and Tukey test (p ? 0.05). Results: After application of rosemary extract, with exception of the E. faecalis biofilm, significant reductions in mono- and polymicrobial biofilms viability were observed. Conclusion: C. albicans, S. aureus, S. mutans and P. aeruginosa monomicrobial biofilms were affected by rosemary extract, as well as C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms, presenting significant viability reductions.   Institute of Science and Technology of São José dos Campos2017-02-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfimage/jpegimage/jpegimage/jpegimage/jpegimage/jpeghttps://ojs.ict.unesp.br/index.php/cob/article/view/131710.14295/bds.2017.v20i1.1317Brazilian Dental Science; Vol. 20 No. 1 (2017): Jan. - Mar. / 2017 - Published February 2017; 64-69Brazilian Dental Science; v. 20 n. 1 (2017): Jan. - Mar. / 2017 - Published February 2017; 64-692178-6011reponame:Brazilian Dental Scienceinstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESPenghttps://ojs.ict.unesp.br/index.php/cob/article/view/1317/1103https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2811https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2812https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2813https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2814https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2815https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2816https://ojs.ict.unesp.br/index.php/cob/article/view/1317/2817Copyright (c) 2017 Brazilian Dental Scienceinfo:eu-repo/semantics/openAccessde Oliveira, Jonatas RafaeJesus, Daiane deOliveira, Luciane Dias de2020-01-28T12:15:37Zoai:ojs.pkp.sfu.ca:article/1317Revistahttp://bds.ict.unesp.br/PUBhttp://ojs.fosjc.unesp.br/index.php/index/oaisergio@fosjc.unesp.br||sergio@fosjc.unesp.br2178-60112178-6011opendoar:2022-11-08T16:30:09.277620Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)true
dc.title.none.fl_str_mv Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest
title Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest
spellingShingle Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest
de Oliveira, Jonatas Rafae
title_short Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest
title_full Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest
title_fullStr Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest
title_full_unstemmed Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest
title_sort Rosmarinus officinalis L. (rosemary) extract decreases the biofilms viability of oral health interest
author de Oliveira, Jonatas Rafae
author_facet de Oliveira, Jonatas Rafae
Jesus, Daiane de
Oliveira, Luciane Dias de
author_role author
author2 Jesus, Daiane de
Oliveira, Luciane Dias de
author2_role author
author
dc.contributor.author.fl_str_mv de Oliveira, Jonatas Rafae
Jesus, Daiane de
Oliveira, Luciane Dias de
description Objective: This study evaluated the effect of rosemary extract on Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and Pseudomonas aeruginosa monomicrobial biofilms viability, as well as on C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms. Material and Methods: In microtiter plate, mono- and polymicrobial biofilms for 48 h were formed. Then, they were exposed for 5 min to rosemary extract (200 mg/mL). Saline (0.9% NaCl) was used as control. After, washes were done with saline to remove the affected cells. Biofilm viability was checked by MTT colorimetric assay, after treatment. Absorbance of the wells was read in microplate spectrophotometer (570 nm) and data were converted to reduction percentage and statistically analyzed by ANOVA and Tukey test (p ? 0.05). Results: After application of rosemary extract, with exception of the E. faecalis biofilm, significant reductions in mono- and polymicrobial biofilms viability were observed. Conclusion: C. albicans, S. aureus, S. mutans and P. aeruginosa monomicrobial biofilms were affected by rosemary extract, as well as C. albicans associated with S. aureus, E. faecalis, S. mutans or P. aeruginosa in polymicrobial biofilms, presenting significant viability reductions.   
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dc.rights.driver.fl_str_mv Copyright (c) 2017 Brazilian Dental Science
info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Institute of Science and Technology of São José dos Campos
publisher.none.fl_str_mv Institute of Science and Technology of São José dos Campos
dc.source.none.fl_str_mv Brazilian Dental Science; Vol. 20 No. 1 (2017): Jan. - Mar. / 2017 - Published February 2017; 64-69
Brazilian Dental Science; v. 20 n. 1 (2017): Jan. - Mar. / 2017 - Published February 2017; 64-69
2178-6011
reponame:Brazilian Dental Science
instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
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instname_str Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
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reponame_str Brazilian Dental Science
collection Brazilian Dental Science
repository.name.fl_str_mv Brazilian Dental Science - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
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