Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Eclética Química |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702006000300008 |
Resumo: | Pothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth rate curve showed that the best growth indices were observed when media 2 submitted to the photoperiod regime was used, followed by the same media kept in darkness (stress condition). The results obtained also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxidase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1 were obtained for kM and v max,, respectively, using guaiacol as enzyme substrate. |
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Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzymePothomorphe umbellata (L.)cell cultureperoxidasePothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth rate curve showed that the best growth indices were observed when media 2 submitted to the photoperiod regime was used, followed by the same media kept in darkness (stress condition). The results obtained also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxidase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1 were obtained for kM and v max,, respectively, using guaiacol as enzyme substrate.Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702006000300008Eclética Química v.31 n.3 2006reponame:Eclética Químicainstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESP10.1590/S0100-46702006000300008info:eu-repo/semantics/openAccessKhalil,N. M.Mello,M. A. M.França,S. C.Oliveira,L. A. A.Oliveira,O. M. M. F.eng2006-12-14T00:00:00Zoai:scielo:S0100-46702006000300008Revistahttp://revista.iq.unesp.br/ojs/index.php/ecletica/PUBhttps://revista.iq.unesp.br/ojs/index.php/ecletica/oaiecletica@ctrlk.com.br||ecletica@iq.unesp.br1678-46181678-4618opendoar:2006-12-14T00:00Eclética Química - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)false |
dc.title.none.fl_str_mv |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
title |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
spellingShingle |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme Khalil,N. M. Pothomorphe umbellata (L.) cell culture peroxidase |
title_short |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
title_full |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
title_fullStr |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
title_full_unstemmed |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
title_sort |
Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme |
author |
Khalil,N. M. |
author_facet |
Khalil,N. M. Mello,M. A. M. França,S. C. Oliveira,L. A. A. Oliveira,O. M. M. F. |
author_role |
author |
author2 |
Mello,M. A. M. França,S. C. Oliveira,L. A. A. Oliveira,O. M. M. F. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Khalil,N. M. Mello,M. A. M. França,S. C. Oliveira,L. A. A. Oliveira,O. M. M. F. |
dc.subject.por.fl_str_mv |
Pothomorphe umbellata (L.) cell culture peroxidase |
topic |
Pothomorphe umbellata (L.) cell culture peroxidase |
description |
Pothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth rate curve showed that the best growth indices were observed when media 2 submitted to the photoperiod regime was used, followed by the same media kept in darkness (stress condition). The results obtained also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxidase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1 were obtained for kM and v max,, respectively, using guaiacol as enzyme substrate. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702006000300008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702006000300008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-46702006000300008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP |
publisher.none.fl_str_mv |
Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP |
dc.source.none.fl_str_mv |
Eclética Química v.31 n.3 2006 reponame:Eclética Química instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Eclética Química |
collection |
Eclética Química |
repository.name.fl_str_mv |
Eclética Química - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) |
repository.mail.fl_str_mv |
ecletica@ctrlk.com.br||ecletica@iq.unesp.br |
_version_ |
1754734618055016448 |