Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme

Detalhes bibliográficos
Autor(a) principal: Khalil,N. M.
Data de Publicação: 2006
Outros Autores: Mello,M. A. M., França,S. C., Oliveira,L. A. A., Oliveira,O. M. M. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Eclética Química
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702006000300008
Resumo: Pothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth rate curve showed that the best growth indices were observed when media 2 submitted to the photoperiod regime was used, followed by the same media kept in darkness (stress condition). The results obtained also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxidase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1 were obtained for kM and v max,, respectively, using guaiacol as enzyme substrate.
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spelling Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzymePothomorphe umbellata (L.)cell cultureperoxidasePothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth rate curve showed that the best growth indices were observed when media 2 submitted to the photoperiod regime was used, followed by the same media kept in darkness (stress condition). The results obtained also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxidase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1 were obtained for kM and v max,, respectively, using guaiacol as enzyme substrate.Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702006000300008Eclética Química v.31 n.3 2006reponame:Eclética Químicainstname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)instacron:UNESP10.1590/S0100-46702006000300008info:eu-repo/semantics/openAccessKhalil,N. M.Mello,M. A. M.França,S. C.Oliveira,L. A. A.Oliveira,O. M. M. F.eng2006-12-14T00:00:00Zoai:scielo:S0100-46702006000300008Revistahttp://revista.iq.unesp.br/ojs/index.php/ecletica/PUBhttps://revista.iq.unesp.br/ojs/index.php/ecletica/oaiecletica@ctrlk.com.br||ecletica@iq.unesp.br1678-46181678-4618opendoar:2006-12-14T00:00Eclética Química - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)false
dc.title.none.fl_str_mv Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
title Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
spellingShingle Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
Khalil,N. M.
Pothomorphe umbellata (L.)
cell culture
peroxidase
title_short Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
title_full Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
title_fullStr Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
title_full_unstemmed Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
title_sort Callus cell culture of Pothomorphe umbellata (L.) under stress condition leads to high content of peroxidase enzyme
author Khalil,N. M.
author_facet Khalil,N. M.
Mello,M. A. M.
França,S. C.
Oliveira,L. A. A.
Oliveira,O. M. M. F.
author_role author
author2 Mello,M. A. M.
França,S. C.
Oliveira,L. A. A.
Oliveira,O. M. M. F.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Khalil,N. M.
Mello,M. A. M.
França,S. C.
Oliveira,L. A. A.
Oliveira,O. M. M. F.
dc.subject.por.fl_str_mv Pothomorphe umbellata (L.)
cell culture
peroxidase
topic Pothomorphe umbellata (L.)
cell culture
peroxidase
description Pothomorphe umbellata (L.) known on Brazil as Caapeba has a number of popular medicinal use, and it has been studied in relation to its pharmacological activity. Peroxidase specific activity (units/mg protein) was evaluated in callus cell culture samples of the P.umbellata, grown in two different MS medium (media 1 and media 2), submitted to 16 hours photoperiod or kept in darkness. Cell growth rate curve showed that the best growth indices were observed when media 2 submitted to the photoperiod regime was used, followed by the same media kept in darkness (stress condition). The results obtained also showed that the cell culture grown under stress conditions (darkness) lead to high content of peroxidase enzyme (an increase of 700% was observed). Kinetic constant values of 3.3 mmol.L-1 and 2,8 sec-1 were obtained for kM and v max,, respectively, using guaiacol as enzyme substrate.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702006000300008
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-46702006000300008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-46702006000300008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP
publisher.none.fl_str_mv Fundação Editora da Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP
dc.source.none.fl_str_mv Eclética Química v.31 n.3 2006
reponame:Eclética Química
instname:Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Eclética Química
collection Eclética Química
repository.name.fl_str_mv Eclética Química - Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
repository.mail.fl_str_mv ecletica@ctrlk.com.br||ecletica@iq.unesp.br
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