Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu

Detalhes bibliográficos
Autor(a) principal: MIRANDA, C. C. B.
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UNIFENAS
Texto Completo: http://tede2.unifenas.br:8080/jspui/handle/jspui/223
Resumo: Potassium (K) is one of the most required nutrients on crops and their availability to plants depends on soil reserves and potassium fertilization. Brazil imports about 90% of K used in agriculture, requiring, therefore, studies related to the discovery of alternative sources of this nutrient, such as use of phonolite rock dust. However, this has slow release of K in the soil, making the utilization unfeasible in agriculture. In this sense, the objectives of this study were to evaluate the potential solubilization of potassium phonolite rock by strains of associative diazotrophs supplied with two carbon sources and verify the feasibility of the use of the phonolite rock powder Brachiaria brizantha cv. Marandu and if the inoculation with bacterial strains diazotrophic and potassium solubilizing contribute to the development of forage. For this, two experiments were conducted, the first one in vitro, in which different bacterial strains were inoculated in Aleksandrov culture modified containing phonolite rock dust. We used two carbon sources, sucrose and glucose to check the effect of these on K. solubilization. The experiment was installed in design completely randomized in a factorial scheme 13 x 2, being 12 bacterial strains and an uninoculated control and two carbon sources. In general, all strains contributed to increase the concentration of K in the culture, highlighting the UNIFENAS 100-13 and 100-94 UNIFENAS strains, which were selected for use in the second experiment, in which these strains were inoculated in Brachiaria brizantha fertilized with different doses of phonolite. This experiment was installed in pots with capacity of 15 soil dm3, using the randomized complete block design (RBD), with treatments arranged in a factorial scheme 6 x 3 x 3, with utilization of KCl and five doses of phonolite: 100, 75, 50 and 25% of the recommended dose of KCl and 3 inoculants variables (UNIFENAS 100-13 strain, UNIFENAS 100-94 strain and control without inoculation), being evaluated by 3 cutting periods. Were evaluated morphological parameters and bromatologics. The use of rock silicate powder phonolite associated with diazotrophs in the development of B. brizantha cv. Marandu promoted better nutritional value as a substitute for potassium chloride ( KCl) . The UNIFENAS 100-94 inculante with the treatment without inoculation had better nutritional value.
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spelling Florentino, Ligiane Aparecida048.747.976-95http://lattes.cnpq.br/3699622468467325REZENDE, Adauton Vilela de583.316.206-82http://lattes.cnpq.br/8772511485766191RUFINI, Márcia076.382.746-01http://lattes.cnpq.br/0183405541192870099.953.976-09http://lattes.cnpq.br/6542678313700066MIRANDA, C. C. B.2019-05-08T17:45:12Z2016-06-03MIRANDA, C. C. B.. Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu. 2016. 68 f. Dissertação( Programa de Mestrado em Ciência Animal) - Universidade José do Rosário Vellano, Alfenas .http://tede2.unifenas.br:8080/jspui/handle/jspui/223Potassium (K) is one of the most required nutrients on crops and their availability to plants depends on soil reserves and potassium fertilization. Brazil imports about 90% of K used in agriculture, requiring, therefore, studies related to the discovery of alternative sources of this nutrient, such as use of phonolite rock dust. However, this has slow release of K in the soil, making the utilization unfeasible in agriculture. In this sense, the objectives of this study were to evaluate the potential solubilization of potassium phonolite rock by strains of associative diazotrophs supplied with two carbon sources and verify the feasibility of the use of the phonolite rock powder Brachiaria brizantha cv. Marandu and if the inoculation with bacterial strains diazotrophic and potassium solubilizing contribute to the development of forage. For this, two experiments were conducted, the first one in vitro, in which different bacterial strains were inoculated in Aleksandrov culture modified containing phonolite rock dust. We used two carbon sources, sucrose and glucose to check the effect of these on K. solubilization. The experiment was installed in design completely randomized in a factorial scheme 13 x 2, being 12 bacterial strains and an uninoculated control and two carbon sources. In general, all strains contributed to increase the concentration of K in the culture, highlighting the UNIFENAS 100-13 and 100-94 UNIFENAS strains, which were selected for use in the second experiment, in which these strains were inoculated in Brachiaria brizantha fertilized with different doses of phonolite. This experiment was installed in pots with capacity of 15 soil dm3, using the randomized complete block design (RBD), with treatments arranged in a factorial scheme 6 x 3 x 3, with utilization of KCl and five doses of phonolite: 100, 75, 50 and 25% of the recommended dose of KCl and 3 inoculants variables (UNIFENAS 100-13 strain, UNIFENAS 100-94 strain and control without inoculation), being evaluated by 3 cutting periods. Were evaluated morphological parameters and bromatologics. The use of rock silicate powder phonolite associated with diazotrophs in the development of B. brizantha cv. Marandu promoted better nutritional value as a substitute for potassium chloride ( KCl) . The UNIFENAS 100-94 inculante with the treatment without inoculation had better nutritional value.O potássio (K) é um dos nutrientes essenciais às culturas e sua disponibilidade para as plantas depende das reservas do solo e aplicação de fertilizantes potássicos. O Brasil importa cerca de 90% do K utilizado na agricultura, necessitando, portanto, de estudos relacionados à descoberta de fontes alternativas deste nutriente, como uso de pó da rocha fonolito. No entanto, este apresenta lenta liberação de K no solo, inviabilizando sua utilização na agricultura. Nesse sentido, os objetivos desse trabalho foram avaliar a solubilização de potássio da rocha fonolito por estirpes de bactérias diazotróficas associativas supridas com duas fontes de carbono e analisar a viabilidade do uso de fonolito em Brachiaria brizantha cv. Marandu e, além disso, verificar se a inoculação com estirpes bacterianas diazotróficas e solubilizadoras de potássio contribuem para o desenvolvimento desta forrageira. Para isso, foram conduzidos dois experimentos; o primeiro, in vitro, em que diferentes estirpes bacterianas foram inoculadas em meio de cultura Aleksandrov modificado contendo pó de rocha fonolito. Foram utilizadas duas fontes de carbono, sacarose e glicose, para verificar o efeito destas na solubilização de K. O experimento foi instalado em delineamento inteiramente casualizado, em esquema fatorial 13 x 2, sendo 12 estirpes bacterianas e um controle sem inoculação e duas fontes de carbono. De um modo geral, todas as estirpes contribuíram para aumentar a concentração de K no meio de cultura, destacando-se as estirpes UNIFENAS 100-13 e UNIFENAS 100-94, as quais foram selecionadas para a utilização no segundo experimento, em que estas estirpes foram inoculadas em B. brizantha adubada com diferentes doses de fonolito. Esse experimento foi instalado em vasos com capacidade de 15 dm3 de solo, utilizando-se o delineamento experimental de blocos ao acaso (DBC) esquema fatorial e um tratamentos adicional (4x3+1), quatro doses do fonolito (Fonolito 100%; Fonolito 75%; Fonolito 50%; Fonolito 25%), 3 variáveis inoculantes (estirpe UNIFENAS 100-13; estirpe UNIFENAS 100-94 e o controle sem inoculação) e KCl (tratamento adicional). Foram avaliados parâmetros morfológicos e bromatológicos. O uso de pó de rocha silicatada fonolito, associado a bactérias diazotróficas no desenvolvimento de B. brizantha cv. Marandu, promoveu melhora no valor nutricional como substituto do cloreto de potássio (KCl). O inculante UNIFENAS 100-94, juntamente com a tratamento sem inoculante, apresentou melhores valores nutricionais.Submitted by Zélia Fernandes Ferreira Miranda (zelia.miranda@unifenas.br) on 2019-05-08T17:45:12Z No. of bitstreams: 1 Cássia Cristina Bachião Miranda.pdf: 1344207 bytes, checksum: d9df5148032fecc391bbeb03c59fb009 (MD5)Made available in DSpace on 2019-05-08T17:45:12Z (GMT). No. of bitstreams: 1 Cássia Cristina Bachião Miranda.pdf: 1344207 bytes, checksum: d9df5148032fecc391bbeb03c59fb009 (MD5) Previous issue date: 2016-06-03Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESFundação de Amparo à Pesquisa do Estado de Minas Gerais - FAPEMIGapplication/pdfporUniversidade José do Rosário VellanoPrograma de Mestrado em Ciência AnimalUNIFENASBrasilPós-GraduaçãoForragem; micro-organismos solubilizadores; rocha potássica.Fodder, solubilizing microorganisms, potassic rock.CIENCIAS AGRARIAS::AGRONOMIABiossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. MaranduBiossolubilization of phonolite by diazotrophic bacteria and potential use in Brachiaria Brizantha VC. 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dc.title.por.fl_str_mv Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu
dc.title.alternative.eng.fl_str_mv Biossolubilization of phonolite by diazotrophic bacteria and potential use in Brachiaria Brizantha VC. Marandu
title Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu
spellingShingle Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu
MIRANDA, C. C. B.
Forragem; micro-organismos solubilizadores; rocha potássica.
Fodder, solubilizing microorganisms, potassic rock.
CIENCIAS AGRARIAS::AGRONOMIA
title_short Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu
title_full Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu
title_fullStr Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu
title_full_unstemmed Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu
title_sort Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu
author MIRANDA, C. C. B.
author_facet MIRANDA, C. C. B.
author_role author
dc.contributor.advisor1.fl_str_mv Florentino, Ligiane Aparecida
dc.contributor.advisor1ID.fl_str_mv 048.747.976-95
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/3699622468467325
dc.contributor.referee1.fl_str_mv REZENDE, Adauton Vilela de
dc.contributor.referee1ID.fl_str_mv 583.316.206-82
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/8772511485766191
dc.contributor.referee2.fl_str_mv RUFINI, Márcia
dc.contributor.referee2ID.fl_str_mv 076.382.746-01
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/0183405541192870
dc.contributor.authorID.fl_str_mv 099.953.976-09
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/6542678313700066
dc.contributor.author.fl_str_mv MIRANDA, C. C. B.
contributor_str_mv Florentino, Ligiane Aparecida
REZENDE, Adauton Vilela de
RUFINI, Márcia
dc.subject.por.fl_str_mv Forragem; micro-organismos solubilizadores; rocha potássica.
topic Forragem; micro-organismos solubilizadores; rocha potássica.
Fodder, solubilizing microorganisms, potassic rock.
CIENCIAS AGRARIAS::AGRONOMIA
dc.subject.eng.fl_str_mv Fodder, solubilizing microorganisms, potassic rock.
dc.subject.cnpq.fl_str_mv CIENCIAS AGRARIAS::AGRONOMIA
description Potassium (K) is one of the most required nutrients on crops and their availability to plants depends on soil reserves and potassium fertilization. Brazil imports about 90% of K used in agriculture, requiring, therefore, studies related to the discovery of alternative sources of this nutrient, such as use of phonolite rock dust. However, this has slow release of K in the soil, making the utilization unfeasible in agriculture. In this sense, the objectives of this study were to evaluate the potential solubilization of potassium phonolite rock by strains of associative diazotrophs supplied with two carbon sources and verify the feasibility of the use of the phonolite rock powder Brachiaria brizantha cv. Marandu and if the inoculation with bacterial strains diazotrophic and potassium solubilizing contribute to the development of forage. For this, two experiments were conducted, the first one in vitro, in which different bacterial strains were inoculated in Aleksandrov culture modified containing phonolite rock dust. We used two carbon sources, sucrose and glucose to check the effect of these on K. solubilization. The experiment was installed in design completely randomized in a factorial scheme 13 x 2, being 12 bacterial strains and an uninoculated control and two carbon sources. In general, all strains contributed to increase the concentration of K in the culture, highlighting the UNIFENAS 100-13 and 100-94 UNIFENAS strains, which were selected for use in the second experiment, in which these strains were inoculated in Brachiaria brizantha fertilized with different doses of phonolite. This experiment was installed in pots with capacity of 15 soil dm3, using the randomized complete block design (RBD), with treatments arranged in a factorial scheme 6 x 3 x 3, with utilization of KCl and five doses of phonolite: 100, 75, 50 and 25% of the recommended dose of KCl and 3 inoculants variables (UNIFENAS 100-13 strain, UNIFENAS 100-94 strain and control without inoculation), being evaluated by 3 cutting periods. Were evaluated morphological parameters and bromatologics. The use of rock silicate powder phonolite associated with diazotrophs in the development of B. brizantha cv. Marandu promoted better nutritional value as a substitute for potassium chloride ( KCl) . The UNIFENAS 100-94 inculante with the treatment without inoculation had better nutritional value.
publishDate 2016
dc.date.issued.fl_str_mv 2016-06-03
dc.date.accessioned.fl_str_mv 2019-05-08T17:45:12Z
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dc.identifier.citation.fl_str_mv MIRANDA, C. C. B.. Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu. 2016. 68 f. Dissertação( Programa de Mestrado em Ciência Animal) - Universidade José do Rosário Vellano, Alfenas .
dc.identifier.uri.fl_str_mv http://tede2.unifenas.br:8080/jspui/handle/jspui/223
identifier_str_mv MIRANDA, C. C. B.. Biossolubilização do fonolito por bactérias diazotróficas e potencial de utilização em Brachiaria Brizantha VC. Marandu. 2016. 68 f. Dissertação( Programa de Mestrado em Ciência Animal) - Universidade José do Rosário Vellano, Alfenas .
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