Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains

Detalhes bibliográficos
Autor(a) principal: Silva, Alexsandra Prudêncio da
Data de Publicação: 2012
Outros Autores: Silva, Adriana Neves da, Santos, Ana Bárbara, Hardoim, Edna Lopes, Batista, Selma Baia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista Brasileira Multidisciplinar
Texto Completo: http://revistarebram.com/index.php/revistauniara/article/view/89
Resumo: Antibiotics are known to have antibacterial, antifungal and antiviral activities that may lead to inhibition or death of the infectious agent. A species of plant that has this characteristic is the garlic, Allium Sativum. Therefore, the aim of this study was to test garlic antibacterial efficiency with different concentration gradients both in the form of watery extract and in natura on a Bacillus thuringiensis berliner. The samples of garlic passed through two methods to obtain the aqueous extract and in natura; at first, the garlic was previously washed with distilled water, triturated and filtered to get concentrations of 30%, 40%, 50% and 60 %; in natura garlic was just macerated. After the incubation period (24, 48 and 72 hours) 43 halos were mensured: 9 with concentration equal to 30%, 10 with 40%, 13 with 50% and 11 halos with a concentration of 60%. The average size of the inhibition halos ranged from 0.4 mm to 40%, 0.9 to 60%. This result shows a probable bacterial efficiency, for the inhibition halo increased in proportion to increasing concentrations, as well as bacteriostatic, for 48 hours in a concentration of 60%. Another conclusion is that the garlic in natura acted as bacteriostatic, although for a longer period, about 72 hours, than on Bacillus thuringiensis. So, this research shows that garlic has a bacterial and bacteriostatic action, but this methodology needs to be adapted to maintain the active principles of antibacterial action for a longer period, both in the garlic extract and in natura.
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spelling Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner StrainsAtividade Antibacteriana do Alho (Allium Sativum Lineu) na Forma de Extrato Aquoso e in Natura Sobre a Cepa Bacillus Thuringiensis BerlinerAllium sativumAntibioticsBacillus thuringiensis.Allium sativumAntibióticosBacillus thuringiensis.Antibiotics are known to have antibacterial, antifungal and antiviral activities that may lead to inhibition or death of the infectious agent. A species of plant that has this characteristic is the garlic, Allium Sativum. Therefore, the aim of this study was to test garlic antibacterial efficiency with different concentration gradients both in the form of watery extract and in natura on a Bacillus thuringiensis berliner. The samples of garlic passed through two methods to obtain the aqueous extract and in natura; at first, the garlic was previously washed with distilled water, triturated and filtered to get concentrations of 30%, 40%, 50% and 60 %; in natura garlic was just macerated. After the incubation period (24, 48 and 72 hours) 43 halos were mensured: 9 with concentration equal to 30%, 10 with 40%, 13 with 50% and 11 halos with a concentration of 60%. The average size of the inhibition halos ranged from 0.4 mm to 40%, 0.9 to 60%. This result shows a probable bacterial efficiency, for the inhibition halo increased in proportion to increasing concentrations, as well as bacteriostatic, for 48 hours in a concentration of 60%. Another conclusion is that the garlic in natura acted as bacteriostatic, although for a longer period, about 72 hours, than on Bacillus thuringiensis. So, this research shows that garlic has a bacterial and bacteriostatic action, but this methodology needs to be adapted to maintain the active principles of antibacterial action for a longer period, both in the garlic extract and in natura.Os antibióticos são conhecidos por apresentarem atividade antibacteriana, antifúngica e antiviral, podendo levar à inibição ou morte de agente infeccioso. Uma espécie de planta que tem esta característica é o alho, Allium sativum. Assim sendo, o objetivo deste trabalho foi testar a eficiência antibacteriana do alho com diferentes gradientes de concentração tanto do extrato aquoso quanto in natura em uma cepa padrão de Bacillus thuringiensis. Para a obtenção do extrato aquoso nas concentrações desejadas (30%, 40%, 50% e 60%), o alho foi previamente lavado com água destilada, triturado e filtrado, enquanto in natura o alho foi apenas macerado. Após o período de incubação (24, 48 e 72 horas) foram mensurados 43 halos de inibição no extrato aquoso, sendo: 9 com concentração de 30%, 10 com 40%, 13 em 50% e 11 halos na concentração de 60%. A média do tamanho desses halos variou de 0,4 mm para 40%, a 0,9 para 60%. O resultado demonstra uma provável eficiência bactericida, pois o halo de inibição aumentou em proporção ao aumento das concentrações, assim como bacteriostática por 48 horas na concentração de 60%. Os resultados usando o alho in natura também demonstraram ação bacteriostática, porém, por um período maior, cerca de 72 horas, sobre o Bacillus thuringiensis. Assim sendo, este trabalho mostra que o alho tem ação tanto bactericida quanto bacteriostática, mas há necessidade de adequação dessa metodologia, a fim de manter os princípios ativos dessas ações por um maior tempo, tanto no extrato do alho quanto in natura.Revista Brasileira Multidisciplinar - ReBraM2012-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistarebram.com/index.php/revistauniara/article/view/8910.25061/2527-2675/ReBraM/2012.v15i2.89Revista Brasileira Multidisciplinar - ReBraM; v. 15 n. 2 (2012): Julho/dezembro; 59-672527-26751415-3580reponame:Revista Brasileira Multidisciplinarinstname:Universidade de Araraquara (UNIARA)instacron:UNIARAporhttp://revistarebram.com/index.php/revistauniara/article/view/89/66Silva, Alexsandra Prudêncio daSilva, Adriana Neves daSantos, Ana BárbaraHardoim, Edna LopesBatista, Selma Baiainfo:eu-repo/semantics/openAccess2018-03-08T14:22:10Zoai:ojs.revistarebram.com:article/89Revistahttp://revistarebram.com/index.php/revistauniaraPRIhttps://revistarebram.com/index.php/revistauniara/oairevistauniara@uniara.com||2527-26752527-2675opendoar:2018-03-08T14:22:10Revista Brasileira Multidisciplinar - Universidade de Araraquara (UNIARA)false
dc.title.none.fl_str_mv Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains
Atividade Antibacteriana do Alho (Allium Sativum Lineu) na Forma de Extrato Aquoso e in Natura Sobre a Cepa Bacillus Thuringiensis Berliner
title Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains
spellingShingle Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains
Silva, Alexsandra Prudêncio da
Allium sativum
Antibiotics
Bacillus thuringiensis.
Allium sativum
Antibióticos
Bacillus thuringiensis.
title_short Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains
title_full Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains
title_fullStr Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains
title_full_unstemmed Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains
title_sort Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains
author Silva, Alexsandra Prudêncio da
author_facet Silva, Alexsandra Prudêncio da
Silva, Adriana Neves da
Santos, Ana Bárbara
Hardoim, Edna Lopes
Batista, Selma Baia
author_role author
author2 Silva, Adriana Neves da
Santos, Ana Bárbara
Hardoim, Edna Lopes
Batista, Selma Baia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Silva, Alexsandra Prudêncio da
Silva, Adriana Neves da
Santos, Ana Bárbara
Hardoim, Edna Lopes
Batista, Selma Baia
dc.subject.por.fl_str_mv Allium sativum
Antibiotics
Bacillus thuringiensis.
Allium sativum
Antibióticos
Bacillus thuringiensis.
topic Allium sativum
Antibiotics
Bacillus thuringiensis.
Allium sativum
Antibióticos
Bacillus thuringiensis.
description Antibiotics are known to have antibacterial, antifungal and antiviral activities that may lead to inhibition or death of the infectious agent. A species of plant that has this characteristic is the garlic, Allium Sativum. Therefore, the aim of this study was to test garlic antibacterial efficiency with different concentration gradients both in the form of watery extract and in natura on a Bacillus thuringiensis berliner. The samples of garlic passed through two methods to obtain the aqueous extract and in natura; at first, the garlic was previously washed with distilled water, triturated and filtered to get concentrations of 30%, 40%, 50% and 60 %; in natura garlic was just macerated. After the incubation period (24, 48 and 72 hours) 43 halos were mensured: 9 with concentration equal to 30%, 10 with 40%, 13 with 50% and 11 halos with a concentration of 60%. The average size of the inhibition halos ranged from 0.4 mm to 40%, 0.9 to 60%. This result shows a probable bacterial efficiency, for the inhibition halo increased in proportion to increasing concentrations, as well as bacteriostatic, for 48 hours in a concentration of 60%. Another conclusion is that the garlic in natura acted as bacteriostatic, although for a longer period, about 72 hours, than on Bacillus thuringiensis. So, this research shows that garlic has a bacterial and bacteriostatic action, but this methodology needs to be adapted to maintain the active principles of antibacterial action for a longer period, both in the garlic extract and in natura.
publishDate 2012
dc.date.none.fl_str_mv 2012-07-06
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://revistarebram.com/index.php/revistauniara/article/view/89
10.25061/2527-2675/ReBraM/2012.v15i2.89
url http://revistarebram.com/index.php/revistauniara/article/view/89
identifier_str_mv 10.25061/2527-2675/ReBraM/2012.v15i2.89
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://revistarebram.com/index.php/revistauniara/article/view/89/66
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Revista Brasileira Multidisciplinar - ReBraM
publisher.none.fl_str_mv Revista Brasileira Multidisciplinar - ReBraM
dc.source.none.fl_str_mv Revista Brasileira Multidisciplinar - ReBraM; v. 15 n. 2 (2012): Julho/dezembro; 59-67
2527-2675
1415-3580
reponame:Revista Brasileira Multidisciplinar
instname:Universidade de Araraquara (UNIARA)
instacron:UNIARA
instname_str Universidade de Araraquara (UNIARA)
instacron_str UNIARA
institution UNIARA
reponame_str Revista Brasileira Multidisciplinar
collection Revista Brasileira Multidisciplinar
repository.name.fl_str_mv Revista Brasileira Multidisciplinar - Universidade de Araraquara (UNIARA)
repository.mail.fl_str_mv revistauniara@uniara.com||
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