Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Brasileira Multidisciplinar |
Texto Completo: | http://revistarebram.com/index.php/revistauniara/article/view/89 |
Resumo: | Antibiotics are known to have antibacterial, antifungal and antiviral activities that may lead to inhibition or death of the infectious agent. A species of plant that has this characteristic is the garlic, Allium Sativum. Therefore, the aim of this study was to test garlic antibacterial efficiency with different concentration gradients both in the form of watery extract and in natura on a Bacillus thuringiensis berliner. The samples of garlic passed through two methods to obtain the aqueous extract and in natura; at first, the garlic was previously washed with distilled water, triturated and filtered to get concentrations of 30%, 40%, 50% and 60 %; in natura garlic was just macerated. After the incubation period (24, 48 and 72 hours) 43 halos were mensured: 9 with concentration equal to 30%, 10 with 40%, 13 with 50% and 11 halos with a concentration of 60%. The average size of the inhibition halos ranged from 0.4 mm to 40%, 0.9 to 60%. This result shows a probable bacterial efficiency, for the inhibition halo increased in proportion to increasing concentrations, as well as bacteriostatic, for 48 hours in a concentration of 60%. Another conclusion is that the garlic in natura acted as bacteriostatic, although for a longer period, about 72 hours, than on Bacillus thuringiensis. So, this research shows that garlic has a bacterial and bacteriostatic action, but this methodology needs to be adapted to maintain the active principles of antibacterial action for a longer period, both in the garlic extract and in natura. |
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Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner StrainsAtividade Antibacteriana do Alho (Allium Sativum Lineu) na Forma de Extrato Aquoso e in Natura Sobre a Cepa Bacillus Thuringiensis BerlinerAllium sativumAntibioticsBacillus thuringiensis.Allium sativumAntibióticosBacillus thuringiensis.Antibiotics are known to have antibacterial, antifungal and antiviral activities that may lead to inhibition or death of the infectious agent. A species of plant that has this characteristic is the garlic, Allium Sativum. Therefore, the aim of this study was to test garlic antibacterial efficiency with different concentration gradients both in the form of watery extract and in natura on a Bacillus thuringiensis berliner. The samples of garlic passed through two methods to obtain the aqueous extract and in natura; at first, the garlic was previously washed with distilled water, triturated and filtered to get concentrations of 30%, 40%, 50% and 60 %; in natura garlic was just macerated. After the incubation period (24, 48 and 72 hours) 43 halos were mensured: 9 with concentration equal to 30%, 10 with 40%, 13 with 50% and 11 halos with a concentration of 60%. The average size of the inhibition halos ranged from 0.4 mm to 40%, 0.9 to 60%. This result shows a probable bacterial efficiency, for the inhibition halo increased in proportion to increasing concentrations, as well as bacteriostatic, for 48 hours in a concentration of 60%. Another conclusion is that the garlic in natura acted as bacteriostatic, although for a longer period, about 72 hours, than on Bacillus thuringiensis. So, this research shows that garlic has a bacterial and bacteriostatic action, but this methodology needs to be adapted to maintain the active principles of antibacterial action for a longer period, both in the garlic extract and in natura.Os antibióticos são conhecidos por apresentarem atividade antibacteriana, antifúngica e antiviral, podendo levar à inibição ou morte de agente infeccioso. Uma espécie de planta que tem esta característica é o alho, Allium sativum. Assim sendo, o objetivo deste trabalho foi testar a eficiência antibacteriana do alho com diferentes gradientes de concentração tanto do extrato aquoso quanto in natura em uma cepa padrão de Bacillus thuringiensis. Para a obtenção do extrato aquoso nas concentrações desejadas (30%, 40%, 50% e 60%), o alho foi previamente lavado com água destilada, triturado e filtrado, enquanto in natura o alho foi apenas macerado. Após o período de incubação (24, 48 e 72 horas) foram mensurados 43 halos de inibição no extrato aquoso, sendo: 9 com concentração de 30%, 10 com 40%, 13 em 50% e 11 halos na concentração de 60%. A média do tamanho desses halos variou de 0,4 mm para 40%, a 0,9 para 60%. O resultado demonstra uma provável eficiência bactericida, pois o halo de inibição aumentou em proporção ao aumento das concentrações, assim como bacteriostática por 48 horas na concentração de 60%. Os resultados usando o alho in natura também demonstraram ação bacteriostática, porém, por um período maior, cerca de 72 horas, sobre o Bacillus thuringiensis. Assim sendo, este trabalho mostra que o alho tem ação tanto bactericida quanto bacteriostática, mas há necessidade de adequação dessa metodologia, a fim de manter os princípios ativos dessas ações por um maior tempo, tanto no extrato do alho quanto in natura.Revista Brasileira Multidisciplinar - ReBraM2012-07-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://revistarebram.com/index.php/revistauniara/article/view/8910.25061/2527-2675/ReBraM/2012.v15i2.89Revista Brasileira Multidisciplinar - ReBraM; v. 15 n. 2 (2012): Julho/dezembro; 59-672527-26751415-3580reponame:Revista Brasileira Multidisciplinarinstname:Universidade de Araraquara (UNIARA)instacron:UNIARAporhttp://revistarebram.com/index.php/revistauniara/article/view/89/66Silva, Alexsandra Prudêncio daSilva, Adriana Neves daSantos, Ana BárbaraHardoim, Edna LopesBatista, Selma Baiainfo:eu-repo/semantics/openAccess2018-03-08T14:22:10Zoai:ojs.revistarebram.com:article/89Revistahttp://revistarebram.com/index.php/revistauniaraPRIhttps://revistarebram.com/index.php/revistauniara/oairevistauniara@uniara.com||2527-26752527-2675opendoar:2018-03-08T14:22:10Revista Brasileira Multidisciplinar - Universidade de Araraquara (UNIARA)false |
dc.title.none.fl_str_mv |
Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains Atividade Antibacteriana do Alho (Allium Sativum Lineu) na Forma de Extrato Aquoso e in Natura Sobre a Cepa Bacillus Thuringiensis Berliner |
title |
Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains |
spellingShingle |
Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains Silva, Alexsandra Prudêncio da Allium sativum Antibiotics Bacillus thuringiensis. Allium sativum Antibióticos Bacillus thuringiensis. |
title_short |
Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains |
title_full |
Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains |
title_fullStr |
Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains |
title_full_unstemmed |
Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains |
title_sort |
Antibacterial Activity of Garlic (Allium Sativum Linnaeus) of Aqueous Extract Form and in Natura on Bacillus Thuringiensis Berliner Strains |
author |
Silva, Alexsandra Prudêncio da |
author_facet |
Silva, Alexsandra Prudêncio da Silva, Adriana Neves da Santos, Ana Bárbara Hardoim, Edna Lopes Batista, Selma Baia |
author_role |
author |
author2 |
Silva, Adriana Neves da Santos, Ana Bárbara Hardoim, Edna Lopes Batista, Selma Baia |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, Alexsandra Prudêncio da Silva, Adriana Neves da Santos, Ana Bárbara Hardoim, Edna Lopes Batista, Selma Baia |
dc.subject.por.fl_str_mv |
Allium sativum Antibiotics Bacillus thuringiensis. Allium sativum Antibióticos Bacillus thuringiensis. |
topic |
Allium sativum Antibiotics Bacillus thuringiensis. Allium sativum Antibióticos Bacillus thuringiensis. |
description |
Antibiotics are known to have antibacterial, antifungal and antiviral activities that may lead to inhibition or death of the infectious agent. A species of plant that has this characteristic is the garlic, Allium Sativum. Therefore, the aim of this study was to test garlic antibacterial efficiency with different concentration gradients both in the form of watery extract and in natura on a Bacillus thuringiensis berliner. The samples of garlic passed through two methods to obtain the aqueous extract and in natura; at first, the garlic was previously washed with distilled water, triturated and filtered to get concentrations of 30%, 40%, 50% and 60 %; in natura garlic was just macerated. After the incubation period (24, 48 and 72 hours) 43 halos were mensured: 9 with concentration equal to 30%, 10 with 40%, 13 with 50% and 11 halos with a concentration of 60%. The average size of the inhibition halos ranged from 0.4 mm to 40%, 0.9 to 60%. This result shows a probable bacterial efficiency, for the inhibition halo increased in proportion to increasing concentrations, as well as bacteriostatic, for 48 hours in a concentration of 60%. Another conclusion is that the garlic in natura acted as bacteriostatic, although for a longer period, about 72 hours, than on Bacillus thuringiensis. So, this research shows that garlic has a bacterial and bacteriostatic action, but this methodology needs to be adapted to maintain the active principles of antibacterial action for a longer period, both in the garlic extract and in natura. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-07-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://revistarebram.com/index.php/revistauniara/article/view/89 10.25061/2527-2675/ReBraM/2012.v15i2.89 |
url |
http://revistarebram.com/index.php/revistauniara/article/view/89 |
identifier_str_mv |
10.25061/2527-2675/ReBraM/2012.v15i2.89 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://revistarebram.com/index.php/revistauniara/article/view/89/66 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Revista Brasileira Multidisciplinar - ReBraM |
publisher.none.fl_str_mv |
Revista Brasileira Multidisciplinar - ReBraM |
dc.source.none.fl_str_mv |
Revista Brasileira Multidisciplinar - ReBraM; v. 15 n. 2 (2012): Julho/dezembro; 59-67 2527-2675 1415-3580 reponame:Revista Brasileira Multidisciplinar instname:Universidade de Araraquara (UNIARA) instacron:UNIARA |
instname_str |
Universidade de Araraquara (UNIARA) |
instacron_str |
UNIARA |
institution |
UNIARA |
reponame_str |
Revista Brasileira Multidisciplinar |
collection |
Revista Brasileira Multidisciplinar |
repository.name.fl_str_mv |
Revista Brasileira Multidisciplinar - Universidade de Araraquara (UNIARA) |
repository.mail.fl_str_mv |
revistauniara@uniara.com|| |
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1797174604901384192 |