Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications

Detalhes bibliográficos
Autor(a) principal: Lima, Gustavo Danilo Nascimento
Data de Publicação: 2021
Outros Autores: Chisini, Luiz Alexandre, Conde, Marcus Cristian Muniz, Faria e Silva, André Luís, Demarco, Flávio Fernando, Ribeiro, Maria Amália Gonzaga
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian journal of oral sciences (Online)
Texto Completo: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8660053
Resumo: Aim: The literature has not yet reported investigations about the effect of laser photobiomodulation (LPBM) over the cytotoxicity of drugs for endodontic treatments. Thus, the aim of this study was to evaluate, in vitro, the effect of the association between LPBM and intracanal medications on fibroblasts viability in different exposure times. Methods: Calcium hydroxide (Ca(OH)2) and iodoform (IO) were used pure or associated to LPBM. Eluates of medications were prepared and placed in contact with the cells in three different periods: 24h, 48h and 72h. Laser irradiation (emitting radiation λ 660nm, power density of 10mW, energy density of 3 J/cm²) has been performed in two sessions within a six hour interval, for 12s per well. After each experimental time, the colorimetric assay (MTT) has been performed. Statistical analysis was applied for Mann-Whitney test with 5% α error admitted test. Results: At 24h, the use of LPBM did not increase cell viability while after 72h cell proliferation was stimulated in the group without medications. LPBM application did not increase cell viability in Ca(OH)2 group and IO at any tested time. Ca(OH)2 cytotoxicity at 24h was higher than iodoform, while at 72h not difference was observed. Therefore, after 72 hours was no statistical difference between the IO and Ca(OH)2 groups. Conclusion: LPBM was able to increase cell viability in 72h in the group without medication, although no improvement was observed in the other groups. Thus, LPBM was not able to reduce the cytotoxic effects of the materials on fibroblasts in vitro.  
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spelling Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medicationsEndodonticsLow-level light therapyFibroblastsAim: The literature has not yet reported investigations about the effect of laser photobiomodulation (LPBM) over the cytotoxicity of drugs for endodontic treatments. Thus, the aim of this study was to evaluate, in vitro, the effect of the association between LPBM and intracanal medications on fibroblasts viability in different exposure times. Methods: Calcium hydroxide (Ca(OH)2) and iodoform (IO) were used pure or associated to LPBM. Eluates of medications were prepared and placed in contact with the cells in three different periods: 24h, 48h and 72h. Laser irradiation (emitting radiation λ 660nm, power density of 10mW, energy density of 3 J/cm²) has been performed in two sessions within a six hour interval, for 12s per well. After each experimental time, the colorimetric assay (MTT) has been performed. Statistical analysis was applied for Mann-Whitney test with 5% α error admitted test. Results: At 24h, the use of LPBM did not increase cell viability while after 72h cell proliferation was stimulated in the group without medications. LPBM application did not increase cell viability in Ca(OH)2 group and IO at any tested time. Ca(OH)2 cytotoxicity at 24h was higher than iodoform, while at 72h not difference was observed. Therefore, after 72 hours was no statistical difference between the IO and Ca(OH)2 groups. Conclusion: LPBM was able to increase cell viability in 72h in the group without medication, although no improvement was observed in the other groups. Thus, LPBM was not able to reduce the cytotoxic effects of the materials on fibroblasts in vitro.  Universidade Estadual de Campinas2021-06-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/otherapplication/pdfhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/866005310.20396/bjos.v20i00.8660053Brazilian Journal of Oral Sciences; v. 20 (2021): Continuous Publication; e210053Brazilian Journal of Oral Sciences; Vol. 20 (2021): Continuous Publication; e2100531677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPenghttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8660053/26903Brazil; ContemporanyCopyright (c) 2021 Brazilian Journal of Oral Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessLima, Gustavo Danilo Nascimento Chisini, Luiz AlexandreConde, Marcus Cristian MunizFaria e Silva, André Luís Demarco, Flávio FernandoRibeiro, Maria Amália Gonzaga 2021-06-15T18:40:44Zoai:ojs.periodicos.sbu.unicamp.br:article/8660053Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2021-06-15T18:40:44Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false
dc.title.none.fl_str_mv Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications
title Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications
spellingShingle Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications
Lima, Gustavo Danilo Nascimento
Endodontics
Low-level light therapy
Fibroblasts
title_short Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications
title_full Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications
title_fullStr Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications
title_full_unstemmed Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications
title_sort Laser photobiomodulation effect on fibroblasts viability exposed to endodontic medications
author Lima, Gustavo Danilo Nascimento
author_facet Lima, Gustavo Danilo Nascimento
Chisini, Luiz Alexandre
Conde, Marcus Cristian Muniz
Faria e Silva, André Luís
Demarco, Flávio Fernando
Ribeiro, Maria Amália Gonzaga
author_role author
author2 Chisini, Luiz Alexandre
Conde, Marcus Cristian Muniz
Faria e Silva, André Luís
Demarco, Flávio Fernando
Ribeiro, Maria Amália Gonzaga
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lima, Gustavo Danilo Nascimento
Chisini, Luiz Alexandre
Conde, Marcus Cristian Muniz
Faria e Silva, André Luís
Demarco, Flávio Fernando
Ribeiro, Maria Amália Gonzaga
dc.subject.por.fl_str_mv Endodontics
Low-level light therapy
Fibroblasts
topic Endodontics
Low-level light therapy
Fibroblasts
description Aim: The literature has not yet reported investigations about the effect of laser photobiomodulation (LPBM) over the cytotoxicity of drugs for endodontic treatments. Thus, the aim of this study was to evaluate, in vitro, the effect of the association between LPBM and intracanal medications on fibroblasts viability in different exposure times. Methods: Calcium hydroxide (Ca(OH)2) and iodoform (IO) were used pure or associated to LPBM. Eluates of medications were prepared and placed in contact with the cells in three different periods: 24h, 48h and 72h. Laser irradiation (emitting radiation λ 660nm, power density of 10mW, energy density of 3 J/cm²) has been performed in two sessions within a six hour interval, for 12s per well. After each experimental time, the colorimetric assay (MTT) has been performed. Statistical analysis was applied for Mann-Whitney test with 5% α error admitted test. Results: At 24h, the use of LPBM did not increase cell viability while after 72h cell proliferation was stimulated in the group without medications. LPBM application did not increase cell viability in Ca(OH)2 group and IO at any tested time. Ca(OH)2 cytotoxicity at 24h was higher than iodoform, while at 72h not difference was observed. Therefore, after 72 hours was no statistical difference between the IO and Ca(OH)2 groups. Conclusion: LPBM was able to increase cell viability in 72h in the group without medication, although no improvement was observed in the other groups. Thus, LPBM was not able to reduce the cytotoxic effects of the materials on fibroblasts in vitro.  
publishDate 2021
dc.date.none.fl_str_mv 2021-06-10
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/other
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8660053
10.20396/bjos.v20i00.8660053
url https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8660053
identifier_str_mv 10.20396/bjos.v20i00.8660053
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8660053/26903
dc.rights.driver.fl_str_mv Copyright (c) 2021 Brazilian Journal of Oral Sciences
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Brazilian Journal of Oral Sciences
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporany
dc.publisher.none.fl_str_mv Universidade Estadual de Campinas
publisher.none.fl_str_mv Universidade Estadual de Campinas
dc.source.none.fl_str_mv Brazilian Journal of Oral Sciences; v. 20 (2021): Continuous Publication; e210053
Brazilian Journal of Oral Sciences; Vol. 20 (2021): Continuous Publication; e210053
1677-3225
reponame:Brazilian journal of oral sciences (Online)
instname:Universidade Estadual de Campinas (UNICAMP)
instacron:UNICAMP
instname_str Universidade Estadual de Campinas (UNICAMP)
instacron_str UNICAMP
institution UNICAMP
reponame_str Brazilian journal of oral sciences (Online)
collection Brazilian journal of oral sciences (Online)
repository.name.fl_str_mv Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)
repository.mail.fl_str_mv brjorals@fop.unicamp.br||brjorals@fop.unicamp.br
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