Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian journal of oral sciences (Online) |
Texto Completo: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640941 |
Resumo: | Aim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge’s criteria (examiner´ Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 ºC for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level. Results: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). Conclusions: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group. |
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Brazilian journal of oral sciences (Online) |
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Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical studyStreptococcus mutansDental cariesDental restorationOdontologiaAim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge’s criteria (examiner´ Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 ºC for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level. Results: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). Conclusions: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group.Universidade Estadual de Campinas2015-10-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640941Brazilian Journal of Oral Sciences; v. 12 n. 4 (2013): Oct./Dec.; 292-297Brazilian Journal of Oral Sciences; Vol. 12 No. 4 (2013): Oct./Dec.; 292-2971677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPporhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640941/8474Copyright (c) 2015 Patricio Vildósola Grez, Patricia Palma Fluxa, Paula Zuñiga Saavedra, Eduardo Fernandez Godoy, Osmir Batista de Oliveira Junior, Gustavo Moncada Cortéshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGrez, Patricio VildósolaFluxa, Patricia PalmaSaavedra, Paula ZuñigaGodoy, Eduardo FernandezOliveira Junior, Osmir Batista deCortés, Gustavo Moncada2023-09-29T15:39:26Zoai:ojs.periodicos.sbu.unicamp.br:article/8640941Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2023-09-29T15:39:26Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
title |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
spellingShingle |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study Grez, Patricio Vildósola Streptococcus mutans Dental caries Dental restoration Odontologia |
title_short |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
title_full |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
title_fullStr |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
title_full_unstemmed |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
title_sort |
Recovering Streptococcus mutans over restorations by the tray technique - a randomized clinical study |
author |
Grez, Patricio Vildósola |
author_facet |
Grez, Patricio Vildósola Fluxa, Patricia Palma Saavedra, Paula Zuñiga Godoy, Eduardo Fernandez Oliveira Junior, Osmir Batista de Cortés, Gustavo Moncada |
author_role |
author |
author2 |
Fluxa, Patricia Palma Saavedra, Paula Zuñiga Godoy, Eduardo Fernandez Oliveira Junior, Osmir Batista de Cortés, Gustavo Moncada |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Grez, Patricio Vildósola Fluxa, Patricia Palma Saavedra, Paula Zuñiga Godoy, Eduardo Fernandez Oliveira Junior, Osmir Batista de Cortés, Gustavo Moncada |
dc.subject.por.fl_str_mv |
Streptococcus mutans Dental caries Dental restoration Odontologia |
topic |
Streptococcus mutans Dental caries Dental restoration Odontologia |
description |
Aim: To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique. Methods: Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were: occlusal restoration evaluated as alpha, according to the Ryge’s criteria (examiner´ Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 ºC for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level. Results: TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47). Conclusions: TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640941 |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640941 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640941/8474 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Oral Sciences; v. 12 n. 4 (2013): Oct./Dec.; 292-297 Brazilian Journal of Oral Sciences; Vol. 12 No. 4 (2013): Oct./Dec.; 292-297 1677-3225 reponame:Brazilian journal of oral sciences (Online) instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Brazilian journal of oral sciences (Online) |
collection |
Brazilian journal of oral sciences (Online) |
repository.name.fl_str_mv |
Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
brjorals@fop.unicamp.br||brjorals@fop.unicamp.br |
_version_ |
1800216398173044736 |