Using molecular markers to assess Streptococcusmutans variability and the biological risk for caries
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian journal of oral sciences (Online) |
Texto Completo: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640894 |
Resumo: | Aim: To characterize the genetic variability of Streptococcus mutans isolates and to correlate this variability with different colonization profiles observed during dental caries in a sample of children. Methods: S. mutans samples were isolated from the saliva of 30 children with varying histories of dental caries, and they were characterized according to morphological and biochemical markers and the sequences of their 16S-23S intergenic spacer region. The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. Next, the isolates were differentiated by sequencing a specific region of the gene encoding the enzyme glucosyltransferase B (gtfB). Results: Characterization using RAPD markers uncovered significant genetic variability among the samples and indicated the existence of clusters, which allowed us to reconstruct both the origin and clinical history of the disease. By sequencing the 16S-23S intergenic region, it was found that all of the isolates belonged to the species S. mutans. Based on the genetic similarity of the isolates and pattern of amino acid variations identified by partial sequencing of the gtfB gene, base-pair changes were identified and correlated with different virulence patterns among the isolates. Conclusions: The partial sequencing of the gtfB gene can be a useful tool for elucidating the colonization patterns of S. mutans. As amino acid variations are likely to be correlated with differences in biological risk, molecular characterization, such as that described in this paper, could be the key for assessing the development of dental caries in children. |
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UNICAMP-8 |
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Brazilian journal of oral sciences (Online) |
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Using molecular markers to assess Streptococcusmutans variability and the biological risk for cariesStreptococcus mutansDental cariesRandom amplified polymorphic DNA techniqueGlucosyltransferaseOdontologiaAim: To characterize the genetic variability of Streptococcus mutans isolates and to correlate this variability with different colonization profiles observed during dental caries in a sample of children. Methods: S. mutans samples were isolated from the saliva of 30 children with varying histories of dental caries, and they were characterized according to morphological and biochemical markers and the sequences of their 16S-23S intergenic spacer region. The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. Next, the isolates were differentiated by sequencing a specific region of the gene encoding the enzyme glucosyltransferase B (gtfB). Results: Characterization using RAPD markers uncovered significant genetic variability among the samples and indicated the existence of clusters, which allowed us to reconstruct both the origin and clinical history of the disease. By sequencing the 16S-23S intergenic region, it was found that all of the isolates belonged to the species S. mutans. Based on the genetic similarity of the isolates and pattern of amino acid variations identified by partial sequencing of the gtfB gene, base-pair changes were identified and correlated with different virulence patterns among the isolates. Conclusions: The partial sequencing of the gtfB gene can be a useful tool for elucidating the colonization patterns of S. mutans. As amino acid variations are likely to be correlated with differences in biological risk, molecular characterization, such as that described in this paper, could be the key for assessing the development of dental caries in children.Universidade Estadual de Campinas2010-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640894Brazilian Journal of Oral Sciences; v. 13 n. 3 (2014): Jul./Set.; 235-241Brazilian Journal of Oral Sciences; Vol. 13 No. 3 (2014): Jul./Set.; 235-2411677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPporhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640894/8432Copyright (c) 2015 Ivana Froede Neiva, Mônica Moreira, Renata Rodrigues Gomes, Debora Klisiowicz, Ricardo Lehtonen Rodrigues Souza, Vânia Aparecida Vicentehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessNeiva, Ivana FroedeMoreira, MônicaGomes, Renata RodriguesKlisiowicz, DeboraSouza, Ricardo Lehtonen RodriguesVicente, Vânia Aparecida2023-10-02T11:50:12Zoai:ojs.periodicos.sbu.unicamp.br:article/8640894Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2023-10-02T11:50:12Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
Using molecular markers to assess Streptococcusmutans variability and the biological risk for caries |
title |
Using molecular markers to assess Streptococcusmutans variability and the biological risk for caries |
spellingShingle |
Using molecular markers to assess Streptococcusmutans variability and the biological risk for caries Neiva, Ivana Froede Streptococcus mutans Dental caries Random amplified polymorphic DNA technique Glucosyltransferase Odontologia |
title_short |
Using molecular markers to assess Streptococcusmutans variability and the biological risk for caries |
title_full |
Using molecular markers to assess Streptococcusmutans variability and the biological risk for caries |
title_fullStr |
Using molecular markers to assess Streptococcusmutans variability and the biological risk for caries |
title_full_unstemmed |
Using molecular markers to assess Streptococcusmutans variability and the biological risk for caries |
title_sort |
Using molecular markers to assess Streptococcusmutans variability and the biological risk for caries |
author |
Neiva, Ivana Froede |
author_facet |
Neiva, Ivana Froede Moreira, Mônica Gomes, Renata Rodrigues Klisiowicz, Debora Souza, Ricardo Lehtonen Rodrigues Vicente, Vânia Aparecida |
author_role |
author |
author2 |
Moreira, Mônica Gomes, Renata Rodrigues Klisiowicz, Debora Souza, Ricardo Lehtonen Rodrigues Vicente, Vânia Aparecida |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Neiva, Ivana Froede Moreira, Mônica Gomes, Renata Rodrigues Klisiowicz, Debora Souza, Ricardo Lehtonen Rodrigues Vicente, Vânia Aparecida |
dc.subject.por.fl_str_mv |
Streptococcus mutans Dental caries Random amplified polymorphic DNA technique Glucosyltransferase Odontologia |
topic |
Streptococcus mutans Dental caries Random amplified polymorphic DNA technique Glucosyltransferase Odontologia |
description |
Aim: To characterize the genetic variability of Streptococcus mutans isolates and to correlate this variability with different colonization profiles observed during dental caries in a sample of children. Methods: S. mutans samples were isolated from the saliva of 30 children with varying histories of dental caries, and they were characterized according to morphological and biochemical markers and the sequences of their 16S-23S intergenic spacer region. The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. Next, the isolates were differentiated by sequencing a specific region of the gene encoding the enzyme glucosyltransferase B (gtfB). Results: Characterization using RAPD markers uncovered significant genetic variability among the samples and indicated the existence of clusters, which allowed us to reconstruct both the origin and clinical history of the disease. By sequencing the 16S-23S intergenic region, it was found that all of the isolates belonged to the species S. mutans. Based on the genetic similarity of the isolates and pattern of amino acid variations identified by partial sequencing of the gtfB gene, base-pair changes were identified and correlated with different virulence patterns among the isolates. Conclusions: The partial sequencing of the gtfB gene can be a useful tool for elucidating the colonization patterns of S. mutans. As amino acid variations are likely to be correlated with differences in biological risk, molecular characterization, such as that described in this paper, could be the key for assessing the development of dental caries in children. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640894 |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640894 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8640894/8432 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Oral Sciences; v. 13 n. 3 (2014): Jul./Set.; 235-241 Brazilian Journal of Oral Sciences; Vol. 13 No. 3 (2014): Jul./Set.; 235-241 1677-3225 reponame:Brazilian journal of oral sciences (Online) instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Brazilian journal of oral sciences (Online) |
collection |
Brazilian journal of oral sciences (Online) |
repository.name.fl_str_mv |
Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
brjorals@fop.unicamp.br||brjorals@fop.unicamp.br |
_version_ |
1800216398108033024 |