In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian journal of oral sciences (Online) |
Texto Completo: | https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642090 |
Resumo: | Aim: An approach for three-dimensional (3D) bone tissue generation from bone marrow mesenchymal adult stem cells (BMSC-AS) was investigated. Methods: The BMSC-AS cells were induced to differentiate into osteogenic precursors, capable of proliferating, and subsequently differentiating into bone-forming cells. The differentiated cells were seeded on the surface of coral discs with a mean diameter 10 (±2) mm and a mean thickness 1 (±0.5) mm. The seeded scaffolds were characterized using von Kossa and Alizarin Red staining, electron and confocal microscopy and RT-PCR analysis. Results: The results demonstrated that BMSC-AS derived bone-forming cells attached to and colonized into coral scaffolds. Furthermore, these cells produced bone nodules when grown for 3-4 weeks in mineralization medium containing ascorbic acid and beta-glycerophosphate both in tissue culture plates and in scaffolds. The differentiated cells also expressed osteospecific markers when grown both in the culture plates and in 3D scaffolds. Osteogenic cells expressed alkaline phosphatase, osteocalcin, and osteopontin, but not a BMSC-AS cell-specific marker, oct-4. Conclusion: These findings suggest that Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) with BMSC-AS cells can be used for in vitro tissue engineering to cultivation of graftable skeletal structures. |
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Brazilian journal of oral sciences (Online) |
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In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary studyIn vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary studyIn vitroCoral reefCORAGRAF3 D scaffoldsBone tissue engineeringOdontologyIn vitroCoral reefCORAGRAF3 D scaffoldsBone tissue engineeringOdontologyAim: An approach for three-dimensional (3D) bone tissue generation from bone marrow mesenchymal adult stem cells (BMSC-AS) was investigated. Methods: The BMSC-AS cells were induced to differentiate into osteogenic precursors, capable of proliferating, and subsequently differentiating into bone-forming cells. The differentiated cells were seeded on the surface of coral discs with a mean diameter 10 (±2) mm and a mean thickness 1 (±0.5) mm. The seeded scaffolds were characterized using von Kossa and Alizarin Red staining, electron and confocal microscopy and RT-PCR analysis. Results: The results demonstrated that BMSC-AS derived bone-forming cells attached to and colonized into coral scaffolds. Furthermore, these cells produced bone nodules when grown for 3-4 weeks in mineralization medium containing ascorbic acid and beta-glycerophosphate both in tissue culture plates and in scaffolds. The differentiated cells also expressed osteospecific markers when grown both in the culture plates and in 3D scaffolds. Osteogenic cells expressed alkaline phosphatase, osteocalcin, and osteopontin, but not a BMSC-AS cell-specific marker, oct-4. Conclusion: These findings suggest that Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) with BMSC-AS cells can be used for in vitro tissue engineering to cultivation of graftable skeletal structures.Aim: An approach for three-dimensional (3D) bone tissue generation from bone marrow mesenchymal adult stem cells (BMSC-AS) was investigated. Methods: The BMSC-AS cells were induced to differentiate into osteogenic precursors, capable of proliferating, and subsequently differentiating into bone-forming cells. The differentiated cells were seeded on the surface of coral discs with a mean diameter 10 (±2) mm and a mean thickness 1 (±0.5) mm. The seeded scaffolds were characterized using von Kossa and Alizarin Red staining, electron and confocal microscopy and RT-PCR analysis. Results: The results demonstrated that BMSC-AS derived bone-forming cells attached to and colonized into coral scaffolds. Furthermore, these cells produced bone nodules when grown for 3-4 weeks in mineralization medium containing ascorbic acid and beta-glycerophosphate both in tissue culture plates and in scaffolds. The differentiated cells also expressed osteospecific markers when grown both in the culture plates and in 3D scaffolds. Osteogenic cells expressed alkaline phosphatase, osteocalcin, and osteopontin, but not a BMSC-AS cell-specific marker, oct-4. Conclusion: These findings suggest that Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) with BMSC-AS cells can be used for in vitro tissue engineering to cultivation of graftable skeletal structures.Universidade Estadual de Campinas2015-12-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/864209010.20396/bjos.v8i4.8642090Brazilian Journal of Oral Sciences; v. 8 n. 4 (2009): Oct./Dec.; 210-216Brazilian Journal of Oral Sciences; Vol. 8 No. 4 (2009): Oct./Dec.; 210-2161677-3225reponame:Brazilian journal of oral sciences (Online)instname:Universidade Estadual de Campinas (UNICAMP)instacron:UNICAMPenghttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642090/9581AL-Salihi, K. A.info:eu-repo/semantics/openAccess2016-02-25T09:13:28Zoai:ojs.periodicos.sbu.unicamp.br:article/8642090Revistahttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/PUBhttps://periodicos.sbu.unicamp.br/ojs/index.php/bjos/oaibrjorals@fop.unicamp.br||brjorals@fop.unicamp.br1677-32251677-3217opendoar:2016-02-25T09:13:28Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP)false |
dc.title.none.fl_str_mv |
In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
title |
In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
spellingShingle |
In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study AL-Salihi, K. A. In vitro Coral reef CORAGRAF 3 D scaffolds Bone tissue engineering Odontology In vitro Coral reef CORAGRAF 3 D scaffolds Bone tissue engineering Odontology |
title_short |
In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
title_full |
In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
title_fullStr |
In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
title_full_unstemmed |
In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
title_sort |
In vitro evaluation of Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) for bone tissue engineering: a preliminary study |
author |
AL-Salihi, K. A. |
author_facet |
AL-Salihi, K. A. |
author_role |
author |
dc.contributor.author.fl_str_mv |
AL-Salihi, K. A. |
dc.subject.por.fl_str_mv |
In vitro Coral reef CORAGRAF 3 D scaffolds Bone tissue engineering Odontology In vitro Coral reef CORAGRAF 3 D scaffolds Bone tissue engineering Odontology |
topic |
In vitro Coral reef CORAGRAF 3 D scaffolds Bone tissue engineering Odontology In vitro Coral reef CORAGRAF 3 D scaffolds Bone tissue engineering Odontology |
description |
Aim: An approach for three-dimensional (3D) bone tissue generation from bone marrow mesenchymal adult stem cells (BMSC-AS) was investigated. Methods: The BMSC-AS cells were induced to differentiate into osteogenic precursors, capable of proliferating, and subsequently differentiating into bone-forming cells. The differentiated cells were seeded on the surface of coral discs with a mean diameter 10 (±2) mm and a mean thickness 1 (±0.5) mm. The seeded scaffolds were characterized using von Kossa and Alizarin Red staining, electron and confocal microscopy and RT-PCR analysis. Results: The results demonstrated that BMSC-AS derived bone-forming cells attached to and colonized into coral scaffolds. Furthermore, these cells produced bone nodules when grown for 3-4 weeks in mineralization medium containing ascorbic acid and beta-glycerophosphate both in tissue culture plates and in scaffolds. The differentiated cells also expressed osteospecific markers when grown both in the culture plates and in 3D scaffolds. Osteogenic cells expressed alkaline phosphatase, osteocalcin, and osteopontin, but not a BMSC-AS cell-specific marker, oct-4. Conclusion: These findings suggest that Malaysian Natural coral Porites bone graft substitutes (CORAGRAF) with BMSC-AS cells can be used for in vitro tissue engineering to cultivation of graftable skeletal structures. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642090 10.20396/bjos.v8i4.8642090 |
url |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642090 |
identifier_str_mv |
10.20396/bjos.v8i4.8642090 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8642090/9581 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
publisher.none.fl_str_mv |
Universidade Estadual de Campinas |
dc.source.none.fl_str_mv |
Brazilian Journal of Oral Sciences; v. 8 n. 4 (2009): Oct./Dec.; 210-216 Brazilian Journal of Oral Sciences; Vol. 8 No. 4 (2009): Oct./Dec.; 210-216 1677-3225 reponame:Brazilian journal of oral sciences (Online) instname:Universidade Estadual de Campinas (UNICAMP) instacron:UNICAMP |
instname_str |
Universidade Estadual de Campinas (UNICAMP) |
instacron_str |
UNICAMP |
institution |
UNICAMP |
reponame_str |
Brazilian journal of oral sciences (Online) |
collection |
Brazilian journal of oral sciences (Online) |
repository.name.fl_str_mv |
Brazilian journal of oral sciences (Online) - Universidade Estadual de Campinas (UNICAMP) |
repository.mail.fl_str_mv |
brjorals@fop.unicamp.br||brjorals@fop.unicamp.br |
_version_ |
1800216400640344064 |