Antifungal activity of Sideroxylon obtusifolium against different species of Candida sp.

Detalhes bibliográficos
Autor(a) principal: da Silva, Renata Soares
Data de Publicação: 2017
Outros Autores: de Oliveira, Karla Maria Santos, Cavalcante, Giani Maria
Tipo de documento: Artigo
Idioma: por
Título da fonte: Estação Científica (UNIFAP)
Texto Completo: https://periodicos.unifap.br/index.php/estacao/article/view/3479
Resumo: Candidiasis is the most common and important opportunistic fungal infection with prevalence in the world is 6.9 cases per 1000 patients. The increase use of antifungal drugs in recent years, it has caused drug resistance available for the treatment. The aim of this study was investigate the antifungal activity of Sideroxylon obtusifolium against strains associated candidiasis. In vitro tests were conducted with crude extract and fractions organics against strains of Candida albicans (URM 4385), Candida glabrata (URM 4264) e Candida tropicalis (URM 4262), a courtesy of Federal University of Pernambuco (UFPE), through in agar-well diffusion assay and minimal inhibitory concentration (MIC). The extract ethanolic and fraction organic methanolic were the most active, with diameter of inhibition zones ranging between 24.5-19.5 mm and 25.0-18.0mm, respectively, values similar to the standard drug itraconozole. The best values of MIC were to extract ethanolic in 200 µg/mL with values varied from 0.32 to 0.16 mg/mL. Chemical study to extraction and isolation of actives compounds is recommended to in vitro assays these compounds to investigate the antimicrobial activity.
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spelling Antifungal activity of Sideroxylon obtusifolium against different species of Candida sp.Atividade antifúngica de Sideroxylon obtusifolium frente a diferentes espécies de Candida sp.Sideroxylon obtusifolium. Candidiasis. Antifungal activity.Sideroxylon obtusifolium. Candidíase. Atividade antifúngica.Candidiasis is the most common and important opportunistic fungal infection with prevalence in the world is 6.9 cases per 1000 patients. The increase use of antifungal drugs in recent years, it has caused drug resistance available for the treatment. The aim of this study was investigate the antifungal activity of Sideroxylon obtusifolium against strains associated candidiasis. In vitro tests were conducted with crude extract and fractions organics against strains of Candida albicans (URM 4385), Candida glabrata (URM 4264) e Candida tropicalis (URM 4262), a courtesy of Federal University of Pernambuco (UFPE), through in agar-well diffusion assay and minimal inhibitory concentration (MIC). The extract ethanolic and fraction organic methanolic were the most active, with diameter of inhibition zones ranging between 24.5-19.5 mm and 25.0-18.0mm, respectively, values similar to the standard drug itraconozole. The best values of MIC were to extract ethanolic in 200 µg/mL with values varied from 0.32 to 0.16 mg/mL. Chemical study to extraction and isolation of actives compounds is recommended to in vitro assays these compounds to investigate the antimicrobial activity.Candidiase é uma infecção fúngica oportunista cuja prevalência no mundo é de 6,9 casos por 1000 pacientes. O aumento na utilização de drogas antifúngicas, nos últimos anos, tem causado resistência aos medicamentos disponíveis para o tratamento. O objetivo deste estudo foi investigar a atividade antifúngica de Sideroxylon obtusifolium em estirpes associadas à candidiase. Os testes in vitro foram realizados com o extrato bruto e frações orgânicas contra estirpes de Candida albicans (URM 4385), Candida glabrata (URM 4264) e Candida tropicalis (URM 4262), gentilmente cedidas pela Micoteca da Universidade Federal de Pernambuco (UFPE), através do ensaio de difusão em ágar com poço e determinação da concentração inibitória mínima (CIM).  O extrato etanólico e a fração metanólica das folhas S. obtusifolium apresentaram potencial antifúngico, com diâmetro dos halos de inibição variando entre 24,5-19,5 mm e 25,0-18,0mm, respectivamente, valores semelhantes à droga padrão itraconozol. Os melhores resultados de CIM foram registrados para o extrato etanólico das folhas de S. obtusifolium na concentração de 200 µg/mL, com valores de CIM variando de 0,32 a 0,16 mg/mL. Estudo químico para a extração e o isolamento dos compostos ativos é recomendado para ensaios in vitro destes compostos para investigar a sua atividade antifúngica.Universidade Federal do Amapáda Silva, Renata Soaresde Oliveira, Karla Maria SantosCavalcante, Giani Maria2017-08-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.unifap.br/index.php/estacao/article/view/347910.18468/estcien.2017v7n1.p95-102Estação Científica (UNIFAP); v. 7, n. 1 (2017); 95-102Estação Científica (UNIFAP); v. 7, n. 1 (2017); 95-1022179-1902reponame:Estação Científica (UNIFAP)instname:Universidade Federal do Amapá (UNIFAP)instacron:UNIFAPporhttps://periodicos.unifap.br/index.php/estacao/article/view/3479/renatav7n1.pdfDireitos autorais 2017 Estação Científica (UNIFAP)http://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess2017-08-21T08:04:48Zoai:ojs.pkp.sfu.ca:article/3479Revistahttps://periodicos.unifap.br/index.php/estacaoONGhttps://periodicos.unifap.br/index.php/estacao/oai||fernandogentry@hotmail.com2179-19022179-1902opendoar:2017-08-21T08:04:48Estação Científica (UNIFAP) - Universidade Federal do Amapá (UNIFAP)false
dc.title.none.fl_str_mv Antifungal activity of Sideroxylon obtusifolium against different species of Candida sp.
Atividade antifúngica de Sideroxylon obtusifolium frente a diferentes espécies de Candida sp.
title Antifungal activity of Sideroxylon obtusifolium against different species of Candida sp.
spellingShingle Antifungal activity of Sideroxylon obtusifolium against different species of Candida sp.
da Silva, Renata Soares
Sideroxylon obtusifolium. Candidiasis. Antifungal activity.
Sideroxylon obtusifolium. Candidíase. Atividade antifúngica.
title_short Antifungal activity of Sideroxylon obtusifolium against different species of Candida sp.
title_full Antifungal activity of Sideroxylon obtusifolium against different species of Candida sp.
title_fullStr Antifungal activity of Sideroxylon obtusifolium against different species of Candida sp.
title_full_unstemmed Antifungal activity of Sideroxylon obtusifolium against different species of Candida sp.
title_sort Antifungal activity of Sideroxylon obtusifolium against different species of Candida sp.
author da Silva, Renata Soares
author_facet da Silva, Renata Soares
de Oliveira, Karla Maria Santos
Cavalcante, Giani Maria
author_role author
author2 de Oliveira, Karla Maria Santos
Cavalcante, Giani Maria
author2_role author
author
dc.contributor.none.fl_str_mv

dc.contributor.author.fl_str_mv da Silva, Renata Soares
de Oliveira, Karla Maria Santos
Cavalcante, Giani Maria
dc.subject.por.fl_str_mv Sideroxylon obtusifolium. Candidiasis. Antifungal activity.
Sideroxylon obtusifolium. Candidíase. Atividade antifúngica.
topic Sideroxylon obtusifolium. Candidiasis. Antifungal activity.
Sideroxylon obtusifolium. Candidíase. Atividade antifúngica.
description Candidiasis is the most common and important opportunistic fungal infection with prevalence in the world is 6.9 cases per 1000 patients. The increase use of antifungal drugs in recent years, it has caused drug resistance available for the treatment. The aim of this study was investigate the antifungal activity of Sideroxylon obtusifolium against strains associated candidiasis. In vitro tests were conducted with crude extract and fractions organics against strains of Candida albicans (URM 4385), Candida glabrata (URM 4264) e Candida tropicalis (URM 4262), a courtesy of Federal University of Pernambuco (UFPE), through in agar-well diffusion assay and minimal inhibitory concentration (MIC). The extract ethanolic and fraction organic methanolic were the most active, with diameter of inhibition zones ranging between 24.5-19.5 mm and 25.0-18.0mm, respectively, values similar to the standard drug itraconozole. The best values of MIC were to extract ethanolic in 200 µg/mL with values varied from 0.32 to 0.16 mg/mL. Chemical study to extraction and isolation of actives compounds is recommended to in vitro assays these compounds to investigate the antimicrobial activity.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-15
dc.type.none.fl_str_mv

dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
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dc.identifier.uri.fl_str_mv https://periodicos.unifap.br/index.php/estacao/article/view/3479
10.18468/estcien.2017v7n1.p95-102
url https://periodicos.unifap.br/index.php/estacao/article/view/3479
identifier_str_mv 10.18468/estcien.2017v7n1.p95-102
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.unifap.br/index.php/estacao/article/view/3479/renatav7n1.pdf
dc.rights.driver.fl_str_mv Direitos autorais 2017 Estação Científica (UNIFAP)
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2017 Estação Científica (UNIFAP)
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal do Amapá
publisher.none.fl_str_mv Universidade Federal do Amapá
dc.source.none.fl_str_mv Estação Científica (UNIFAP); v. 7, n. 1 (2017); 95-102
Estação Científica (UNIFAP); v. 7, n. 1 (2017); 95-102
2179-1902
reponame:Estação Científica (UNIFAP)
instname:Universidade Federal do Amapá (UNIFAP)
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instname_str Universidade Federal do Amapá (UNIFAP)
instacron_str UNIFAP
institution UNIFAP
reponame_str Estação Científica (UNIFAP)
collection Estação Científica (UNIFAP)
repository.name.fl_str_mv Estação Científica (UNIFAP) - Universidade Federal do Amapá (UNIFAP)
repository.mail.fl_str_mv ||fernandogentry@hotmail.com
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