Bacillus toyonensis SUPPLEMENTATION MODULATES AND IMPROVES THE ANTIBODY PRODUCTION IN MICE IMMUNIZED WITH Leishmania (Leishmania) infatum chagasi ANTIGENS

• Autor(a) principal: Sampaio, Ana Paula Presley Oliveira
• Data de Publicação: 2020
• Outros Autores: Lima, Joelson Sousa, da Silva, Flavio, Monteiro, Maiara Vasconcelos, Bezerra, Isis Abel, Leivas Leite, Fabio Pereira, de Moraes, Carina Martins, Roos, Talita Bandeira
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Biota Amazônia
• Texto Completo: https://periodicos.unifap.br/index.php/biota/article/view/5160
• topic: Leishmaniose canina; probiótico; resposta imunológica.

The aim of this study was to evaluate the effect of supplementation with the probiotic Bacillus toyonensis on the production of IgG, IgG1 and IgG2a antibodies against Leishmania (Leishmania) infantum chagasi antigens. Twenty-four female albino BALB/c mice, 21 days old, were immunized experimentally against L. (L.) infantum chagasi, divided into three experimental groups. Group A received no supplementation, group B was continuously supplemented until day 56 and in group C the probiotic was administered seven days before and seven days after each immunization for the same period. The experiment was conducted until day 84. Seroconversion was used to evaluate the humoral immune response. During the supplementation, all the animals presented total IgG seroconversion against the antigen used, without statistical difference (p0.05) between the groups. In the isotype analysis, the group supplemented with probiotic in the continuous period presented seroconversion results of the upper IgG2a / IgG1 ray when compared to the control group (1.8 times) and to that supplemented seven days before and seven days after supplementation (1.2 times) on day 70, keeping their titre superior to the groups in question until the end of the experiment (1.2 times on day 84). Based on these results, it can observed a greater ability of the supplemented group to continuously modulate favorably the humoral immune response and to maintain the production of IgG2a isotype antibodies against the antigen in question.Keywords: Canine leishmaniasis; probiotic; immune response.