ABSENCE OF CYTOTOXIC AND GENOTOXIC EFFECTS OF THE AQUEOUS EXTRACT OF Stryphnodendron adstringens (BARBATIMÃO) BARK USING Allium cepa TEST

• Autor(a) principal: dos Reis, Herald Souza
• Data de Publicação: 2020
• Outros Autores: da Costa, Mary Helen Pestana, Moraes, Sheyla Michele de Souza, Monteiro, José Augusto do Nascimento, Leão, Diana do Vale, da Rocha, Carlos Alberto Machado
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Biota Amazônia
• Texto Completo: https://periodicos.unifap.br/index.php/biota/article/view/5019
• topic: medicinal plants; folk medicine; toxicity; infusion; ethnobotany

Barbatimão [Stryphnodendron adstringens (Mart.) Coville] is a plant of Mimosaceae family found in several regions of Brazil. Its bark is intensively used in folk medicine, mainly for its healing and antimicrobial actions. Medicinal plants could be dangerous to human health because some of them may have many toxicity products. The aim study was to investigate the cytotoxic and genotoxic effects of aqueous extract from the stem bark of barbatimão through Allium cepa test. The A. cepa seeds were watered with two concentrations (50 and 100 mg.mL-1) of barbatimão extract, a negative control (distilled water) and positive control (MNU solution 0.125 mg.L-1). It were analyzed 4000 cells per treatment and observed the number of cells in each phase of mitosis and changes in the cell cycle. Statistical analysis of the data was performed through analysis of variance (one-way ANOVA), with a probability level 0.05. About the cytotoxic effects, the four groups did not differ in the germination rate (p0.05) but mean values of root length, weight and mitotic index were significantly lower in the positive control group (p0.05). Regarding genotoxic effects, there were no significant differences between the barbatimão extract treatments and the negative control (p0.05). At the positive control, however, there was a significant increase in micronucleus frequency (p=0.0042) and mitotic cycle anomalies (p=0.0076). It was concluded that the barbatimão extract not presented cytotoxic or genotoxic effects.Keywords: medicinal plants; folk medicine; toxicity; infusion; ethnobotany.