Sexing as a tool for reinsertion of Amazona aestiva parrots to nature: use of less invasive technique

Detalhes bibliográficos
Autor(a) principal: Ramos, Carine Novato
Data de Publicação: 2021
Outros Autores: Barros, Tácita Borges, Antonio, Edma Santos de, Mirabal, Bernardo, Silva, Márcio Borba da, Fraga, Ricardo Evangelista, Tomazi, Laize
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/20601
Resumo: The sexing birds is considered an important tool for behavioral studies and programs for the reintroduction of animals into the wild. Several techniques are used for this purpose, such as laparoscopy, magnetic resonance and molecular sexing. The first are considered more invasive and stressful for the animal, and the last is considered the most accurate. According to it, the aim of this study was to compare the effectiveness of using three sets of primers in the molecular sexing process of true parrots (Amazona aestiva). Blood samples from 10 animals were collected at a Wildlife Screening Center (CETAS) in Bahia, Brazil. The DNA was extracted and the molecular markers amplified by Polymerase Chain Reaction (PCR) using primer pairs P2/P8, 1237L/1272H and 2250F/2718R. The amplified material was visualized with electrophoresis performed at 2% agarose and 12.5% polyacrylamide gels. Among the primer sets used, the 2250F/2718R pair showed the best results for the sexing process, including visualization of the amplified products on an agarose gel. Agarose gel electrophoresis is considered to be faster and cheaper. The results revealed a sample composition of 5 males (0.5) and 5 females (0.5).
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spelling Sexing as a tool for reinsertion of Amazona aestiva parrots to nature: use of less invasive techniqueEl sexado como herramienta para la reinserción de los loros Amazona aestiva a la naturaleza: uso de técnica menos invasiveSexagem como ferramenta para reinserção de papagaios Amazona aestiva à natureza: uso de técnica menos invasivaCHD-Z/WPCRSexagem molecularIniciadores 2550F/2718R.CHD-Z/WPCRSexaje molecularCebadores 2550F/2718R.CHD-Z/WPCRMolecular sexingPrimer 2550F/2718R.The sexing birds is considered an important tool for behavioral studies and programs for the reintroduction of animals into the wild. Several techniques are used for this purpose, such as laparoscopy, magnetic resonance and molecular sexing. The first are considered more invasive and stressful for the animal, and the last is considered the most accurate. According to it, the aim of this study was to compare the effectiveness of using three sets of primers in the molecular sexing process of true parrots (Amazona aestiva). Blood samples from 10 animals were collected at a Wildlife Screening Center (CETAS) in Bahia, Brazil. The DNA was extracted and the molecular markers amplified by Polymerase Chain Reaction (PCR) using primer pairs P2/P8, 1237L/1272H and 2250F/2718R. The amplified material was visualized with electrophoresis performed at 2% agarose and 12.5% polyacrylamide gels. Among the primer sets used, the 2250F/2718R pair showed the best results for the sexing process, including visualization of the amplified products on an agarose gel. Agarose gel electrophoresis is considered to be faster and cheaper. The results revealed a sample composition of 5 males (0.5) and 5 females (0.5).El sexaje de aves es considerado una herramienta importante para estudios de comportamiento y programas de reintroducción de animales en el medio natural. Diversas técnicas son utilizadas para este fin, como la laparoscopia, la resonancia magnética y el sexaje molecular. Las primeras son consideradas más invasivas y estresantes para el animal y la última es considerada la más precisa. Ante el expuesto, el presente trabajo tuvo el objetivo de comparar la eficiencia del uso de tres conjuntos de primers en el proceso de sexaje molecular de loros verdaderos (Amazona aestiva). Así, se recogieron muestras sanguíneas de diez animales, en el Centro de Triage de Animales Silvestres (CETAS) del estado de Bahía, Brasil. El ADN fue extraído y los marcadores moleculares amplificados por Reacción en Cadena de la Polimerasa (PCR), utilizando los pares de cebadores: P2/ P8, 1237/1272H y 2250F/2718R. Para la visualización del material amplificado se realizó electroforesis en geles de agarosa 2% y de poliacrilamida 12,5%. Entre los conjuntos de cebadores utilizados, el par 2250F/2718R presentó los mejores resultados para el sexaje, incluyendo la visualización de los productos amplificados en gel de agarosa. La electroforesis en gel de agarosa es considerada más rápida y menos costosa. Los resultados revelaron una composición muestral de 5 machos (0,5) y 5 hembras (0,5).A sexagem das aves é considerada uma importante ferramenta para estudos comportamentais e programas de reintrodução de animais na natureza. Diversas técnicas são utilizadas para esse fim, como laparoscopia, ressonância magnética e sexagem molecular. Os primeiros são considerados mais invasivos e estressantes para o animal, e o último é considerado o mais preciso. Tendo isto em vista, o objetivo deste estudo foi comparar a eficácia do uso de três conjuntos de primers no processo de sexagem molecular de papagaios verdadeiros (Amazona aestiva). Amostras de sangue de 10 animais foram coletadas em um Centro de Triagem de Animais Selvagens (CETAS) na Bahia, Brasil. O DNA foi extraído e os marcadores moleculares amplificados por Reação em Cadeia da Polimerase (PCR) utilizando os iniciadores P2/P8, 1237L/1272H e 2250F/2718R. O material amplificado foi visualizado com eletroforese, realizada em géis de agarose 2% e poliacrilamida 12,5%. Dentre os conjuntos de primers utilizados, o par 2250F/2718R apresentou os melhores resultados para o processo de sexagem, incluindo a visualização dos produtos amplificados em gel de agarose. A eletroforese em gel de agarose é considerada mais rápida e barata. Os resultados revelaram uma composição amostral de 5 machos (0,5) e 5 fêmeas (0,5).Research, Society and Development2021-09-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2060110.33448/rsd-v10i12.20601Research, Society and Development; Vol. 10 No. 12; e380101220601Research, Society and Development; Vol. 10 Núm. 12; e380101220601Research, Society and Development; v. 10 n. 12; e3801012206012525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/20601/18341Copyright (c) 2021 Carine Novato Ramos; Tácita Borges Barros; Edma Santos de Antonio; Bernardo Mirabal; Márcio Borba da Silva; Ricardo Evangelista Fraga; Laize Tomazihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRamos, Carine NovatoBarros, Tácita Borges Antonio, Edma Santos deMirabal, BernardoSilva, Márcio Borba daFraga, Ricardo EvangelistaTomazi, Laize2021-11-14T20:26:51Zoai:ojs.pkp.sfu.ca:article/20601Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:40:12.275568Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Sexing as a tool for reinsertion of Amazona aestiva parrots to nature: use of less invasive technique
El sexado como herramienta para la reinserción de los loros Amazona aestiva a la naturaleza: uso de técnica menos invasive
Sexagem como ferramenta para reinserção de papagaios Amazona aestiva à natureza: uso de técnica menos invasiva
title Sexing as a tool for reinsertion of Amazona aestiva parrots to nature: use of less invasive technique
spellingShingle Sexing as a tool for reinsertion of Amazona aestiva parrots to nature: use of less invasive technique
Ramos, Carine Novato
CHD-Z/W
PCR
Sexagem molecular
Iniciadores 2550F/2718R.
CHD-Z/W
PCR
Sexaje molecular
Cebadores 2550F/2718R.
CHD-Z/W
PCR
Molecular sexing
Primer 2550F/2718R.
title_short Sexing as a tool for reinsertion of Amazona aestiva parrots to nature: use of less invasive technique
title_full Sexing as a tool for reinsertion of Amazona aestiva parrots to nature: use of less invasive technique
title_fullStr Sexing as a tool for reinsertion of Amazona aestiva parrots to nature: use of less invasive technique
title_full_unstemmed Sexing as a tool for reinsertion of Amazona aestiva parrots to nature: use of less invasive technique
title_sort Sexing as a tool for reinsertion of Amazona aestiva parrots to nature: use of less invasive technique
author Ramos, Carine Novato
author_facet Ramos, Carine Novato
Barros, Tácita Borges
Antonio, Edma Santos de
Mirabal, Bernardo
Silva, Márcio Borba da
Fraga, Ricardo Evangelista
Tomazi, Laize
author_role author
author2 Barros, Tácita Borges
Antonio, Edma Santos de
Mirabal, Bernardo
Silva, Márcio Borba da
Fraga, Ricardo Evangelista
Tomazi, Laize
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ramos, Carine Novato
Barros, Tácita Borges
Antonio, Edma Santos de
Mirabal, Bernardo
Silva, Márcio Borba da
Fraga, Ricardo Evangelista
Tomazi, Laize
dc.subject.por.fl_str_mv CHD-Z/W
PCR
Sexagem molecular
Iniciadores 2550F/2718R.
CHD-Z/W
PCR
Sexaje molecular
Cebadores 2550F/2718R.
CHD-Z/W
PCR
Molecular sexing
Primer 2550F/2718R.
topic CHD-Z/W
PCR
Sexagem molecular
Iniciadores 2550F/2718R.
CHD-Z/W
PCR
Sexaje molecular
Cebadores 2550F/2718R.
CHD-Z/W
PCR
Molecular sexing
Primer 2550F/2718R.
description The sexing birds is considered an important tool for behavioral studies and programs for the reintroduction of animals into the wild. Several techniques are used for this purpose, such as laparoscopy, magnetic resonance and molecular sexing. The first are considered more invasive and stressful for the animal, and the last is considered the most accurate. According to it, the aim of this study was to compare the effectiveness of using three sets of primers in the molecular sexing process of true parrots (Amazona aestiva). Blood samples from 10 animals were collected at a Wildlife Screening Center (CETAS) in Bahia, Brazil. The DNA was extracted and the molecular markers amplified by Polymerase Chain Reaction (PCR) using primer pairs P2/P8, 1237L/1272H and 2250F/2718R. The amplified material was visualized with electrophoresis performed at 2% agarose and 12.5% polyacrylamide gels. Among the primer sets used, the 2250F/2718R pair showed the best results for the sexing process, including visualization of the amplified products on an agarose gel. Agarose gel electrophoresis is considered to be faster and cheaper. The results revealed a sample composition of 5 males (0.5) and 5 females (0.5).
publishDate 2021
dc.date.none.fl_str_mv 2021-09-24
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/20601
10.33448/rsd-v10i12.20601
url https://rsdjournal.org/index.php/rsd/article/view/20601
identifier_str_mv 10.33448/rsd-v10i12.20601
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/20601/18341
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 12; e380101220601
Research, Society and Development; Vol. 10 Núm. 12; e380101220601
Research, Society and Development; v. 10 n. 12; e380101220601
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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