Chemical composition and biological activity of propolis from Melipona quadrifasciata

Detalhes bibliográficos
Autor(a) principal: Piccinini, Alexandre
Data de Publicação: 2022
Outros Autores: Sousa, Matheus Henrique Oliveira de, Freitas, Marta dos Santos Diniz, Cesca, Karina, Moura, Neusa Fernandes de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/34175
Resumo: Propolis is a viscous product produced and collected by worker bees from shots or plant exudates used for the protection of hive. The aims of this work were to perform the phytochemical profile and biological activity of propolis from stingless bee (SB) Melipona quadrifasciata (Mandaçaia) native from Rio Grande do Sul, Brazil. The propolis had a total phenolic concentration of 126,52 mg g-1, and the major phenolic compounds identified were p-coumaric acid (35,64 mg g-1) and gallic acid (18,33 mg g-1). The carotenoids β-carotene, β-criptoxanthin, lutein and lycopene were identified in the propolis of M. quadrifasciata, and β-carotene showed the highest concentration (1.56 mg/100g). The headspace analysis of volatile compounds identified the highest concentration of α-pinene, followed by limonene, sylvestrene and β-pinene. The propolis of M. quadrifasciata showed high antioxidant activity by the DPPH method, with EC50 of 2,29 µg mL-1, antimicrobial activity (MIC) against Escherichia coli and Staphylococcus aureus and low reduction in cell viability of cell lines L929, GL, A549, MDA and SKMEL.
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spelling Chemical composition and biological activity of propolis from Melipona quadrifasciataComposición química y actividad biológica del propóleo de Melipona quadrifasciataComposição química e atividade biológica da própolis de Melipona quadrifasciataMeliponinesStingless beePhenolicsCarotenoidsVolatile compounds.MeliponinasAbeja sin aguijónFenólicosCarotenoidesCompuestos volátiles.MeliponineosAbelha sem ferrãoFenólicosCarotenóidesCompostos voláteis.Propolis is a viscous product produced and collected by worker bees from shots or plant exudates used for the protection of hive. The aims of this work were to perform the phytochemical profile and biological activity of propolis from stingless bee (SB) Melipona quadrifasciata (Mandaçaia) native from Rio Grande do Sul, Brazil. The propolis had a total phenolic concentration of 126,52 mg g-1, and the major phenolic compounds identified were p-coumaric acid (35,64 mg g-1) and gallic acid (18,33 mg g-1). The carotenoids β-carotene, β-criptoxanthin, lutein and lycopene were identified in the propolis of M. quadrifasciata, and β-carotene showed the highest concentration (1.56 mg/100g). The headspace analysis of volatile compounds identified the highest concentration of α-pinene, followed by limonene, sylvestrene and β-pinene. The propolis of M. quadrifasciata showed high antioxidant activity by the DPPH method, with EC50 of 2,29 µg mL-1, antimicrobial activity (MIC) against Escherichia coli and Staphylococcus aureus and low reduction in cell viability of cell lines L929, GL, A549, MDA and SKMEL.El propóleo es un producto viscoso producido y recolectado por abejas obreras, a partir de yemas o exudados de plantas, utilizado para proteger la colmena. El objetivo de este trabajo fue realizar el perfil fitoquímico y la actividad biológica del propóleo de la abeja sin aguijón (ASA) Melipona quadrifasciata (Mandaçaia) originaria de Rio Grande do Sul, Brasil. El propóleo tuvo una concentración fenólica total de 126,52 mg g-1, y los principales compuestos fenólicos identificados fueron ácido p-cumárico (35,64 mg g-1) y ácido gálico (18,33 mg g-1). Los carotenoides β-caroteno, β-criptoxantina, luteína y licopeno fueron identificados en el propóleo de M. quadrifasciata, y el β-caroteno tuvo la mayor concentración (1,56 mg/100g). Los compuestos volátiles se obtuvieron por espacio de cabeza y se identificaron por GC/MS, siendo el α-pineno el compuesto mayoritario, seguido por el limoneno, el silvestreno y el β-pineno. Entre las actividades biológicas realizadas con el propóleo de M. quadrifasciata, mostró alta actividad antirradicalaria por el método DPPH, con EC50 de 2,29 µg mL-1, actividad antimicrobiana (MIC) contra Escherichia coli y Staphylococcus aureus y baja reducción en la viabilidad celular de las líneas celulares L929, GL, A549, MDA y SKMEL.A própolis é um produto viscoso produzido e coletado por abelhas operárias, a partir de brotos ou exsudatos de plantas, utilizados para proteção da colmeia. O objetivo deste trabalho foi realizar o perfil fitoquímico e a atividade biológica da própolis de abelha sem ferrão (ASF) Melipona quadrifasciata (Mandaçaia) nativa do Rio Grande do Sul, Brasil. A própolis apresentou concentração fenólica total de 126,52 mg g-1, e os principais compostos fenólicos identificados foram ácido p-cumárico (35,64 mg g-1) e ácido gálico (18,33 mg g-1). Os carotenóides β-caroteno, β-criptoxantina, luteína e licopeno foram identificados na própolis de M. quadrifasciata, sendo que o β-caroteno apresentou a maior concentração (1,56 mg/100g). Os compostos voláteis foram obtidos por headspace e identificados por CG/EM, sendo o α-pineno o composto majoritário, seguido de limoneno, silvestreno e β-pineno. A própolis apresentou alta atividade antiradicalar pelo método DPPH, com EC50 de 2,29 µg mL-1, atividade antimicrobiana (CIM) contra Escherichia coli e Staphylococcus aureus e baixa redução na viabilidade celular das linhagens celulares L929, GL, A549, MDA e SKMEL.Research, Society and Development2022-09-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3417510.33448/rsd-v11i12.34175Research, Society and Development; Vol. 11 No. 12; e193111234175Research, Society and Development; Vol. 11 Núm. 12; e193111234175Research, Society and Development; v. 11 n. 12; e1931112341752525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/34175/29002Copyright (c) 2022 Alexandre Piccinini; Matheus Henrique Oliveira de Sousa; Marta dos Santos Diniz Freitas; Karina Cesca; Neusa Fernandes de Mourahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPiccinini, AlexandreSousa, Matheus Henrique Oliveira deFreitas, Marta dos Santos DinizCesca, KarinaMoura, Neusa Fernandes de2022-09-26T11:56:08Zoai:ojs.pkp.sfu.ca:article/34175Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:49:34.846155Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Chemical composition and biological activity of propolis from Melipona quadrifasciata
Composición química y actividad biológica del propóleo de Melipona quadrifasciata
Composição química e atividade biológica da própolis de Melipona quadrifasciata
title Chemical composition and biological activity of propolis from Melipona quadrifasciata
spellingShingle Chemical composition and biological activity of propolis from Melipona quadrifasciata
Piccinini, Alexandre
Meliponines
Stingless bee
Phenolics
Carotenoids
Volatile compounds.
Meliponinas
Abeja sin aguijón
Fenólicos
Carotenoides
Compuestos volátiles.
Meliponineos
Abelha sem ferrão
Fenólicos
Carotenóides
Compostos voláteis.
title_short Chemical composition and biological activity of propolis from Melipona quadrifasciata
title_full Chemical composition and biological activity of propolis from Melipona quadrifasciata
title_fullStr Chemical composition and biological activity of propolis from Melipona quadrifasciata
title_full_unstemmed Chemical composition and biological activity of propolis from Melipona quadrifasciata
title_sort Chemical composition and biological activity of propolis from Melipona quadrifasciata
author Piccinini, Alexandre
author_facet Piccinini, Alexandre
Sousa, Matheus Henrique Oliveira de
Freitas, Marta dos Santos Diniz
Cesca, Karina
Moura, Neusa Fernandes de
author_role author
author2 Sousa, Matheus Henrique Oliveira de
Freitas, Marta dos Santos Diniz
Cesca, Karina
Moura, Neusa Fernandes de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Piccinini, Alexandre
Sousa, Matheus Henrique Oliveira de
Freitas, Marta dos Santos Diniz
Cesca, Karina
Moura, Neusa Fernandes de
dc.subject.por.fl_str_mv Meliponines
Stingless bee
Phenolics
Carotenoids
Volatile compounds.
Meliponinas
Abeja sin aguijón
Fenólicos
Carotenoides
Compuestos volátiles.
Meliponineos
Abelha sem ferrão
Fenólicos
Carotenóides
Compostos voláteis.
topic Meliponines
Stingless bee
Phenolics
Carotenoids
Volatile compounds.
Meliponinas
Abeja sin aguijón
Fenólicos
Carotenoides
Compuestos volátiles.
Meliponineos
Abelha sem ferrão
Fenólicos
Carotenóides
Compostos voláteis.
description Propolis is a viscous product produced and collected by worker bees from shots or plant exudates used for the protection of hive. The aims of this work were to perform the phytochemical profile and biological activity of propolis from stingless bee (SB) Melipona quadrifasciata (Mandaçaia) native from Rio Grande do Sul, Brazil. The propolis had a total phenolic concentration of 126,52 mg g-1, and the major phenolic compounds identified were p-coumaric acid (35,64 mg g-1) and gallic acid (18,33 mg g-1). The carotenoids β-carotene, β-criptoxanthin, lutein and lycopene were identified in the propolis of M. quadrifasciata, and β-carotene showed the highest concentration (1.56 mg/100g). The headspace analysis of volatile compounds identified the highest concentration of α-pinene, followed by limonene, sylvestrene and β-pinene. The propolis of M. quadrifasciata showed high antioxidant activity by the DPPH method, with EC50 of 2,29 µg mL-1, antimicrobial activity (MIC) against Escherichia coli and Staphylococcus aureus and low reduction in cell viability of cell lines L929, GL, A549, MDA and SKMEL.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-11
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/34175
10.33448/rsd-v11i12.34175
url https://rsdjournal.org/index.php/rsd/article/view/34175
identifier_str_mv 10.33448/rsd-v11i12.34175
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/34175/29002
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 12; e193111234175
Research, Society and Development; Vol. 11 Núm. 12; e193111234175
Research, Society and Development; v. 11 n. 12; e193111234175
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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