Chemical composition and biological activity of propolis from Melipona quadrifasciata
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/34175 |
Resumo: | Propolis is a viscous product produced and collected by worker bees from shots or plant exudates used for the protection of hive. The aims of this work were to perform the phytochemical profile and biological activity of propolis from stingless bee (SB) Melipona quadrifasciata (Mandaçaia) native from Rio Grande do Sul, Brazil. The propolis had a total phenolic concentration of 126,52 mg g-1, and the major phenolic compounds identified were p-coumaric acid (35,64 mg g-1) and gallic acid (18,33 mg g-1). The carotenoids β-carotene, β-criptoxanthin, lutein and lycopene were identified in the propolis of M. quadrifasciata, and β-carotene showed the highest concentration (1.56 mg/100g). The headspace analysis of volatile compounds identified the highest concentration of α-pinene, followed by limonene, sylvestrene and β-pinene. The propolis of M. quadrifasciata showed high antioxidant activity by the DPPH method, with EC50 of 2,29 µg mL-1, antimicrobial activity (MIC) against Escherichia coli and Staphylococcus aureus and low reduction in cell viability of cell lines L929, GL, A549, MDA and SKMEL. |
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Chemical composition and biological activity of propolis from Melipona quadrifasciataComposición química y actividad biológica del propóleo de Melipona quadrifasciataComposição química e atividade biológica da própolis de Melipona quadrifasciataMeliponinesStingless beePhenolicsCarotenoidsVolatile compounds.MeliponinasAbeja sin aguijónFenólicosCarotenoidesCompuestos volátiles.MeliponineosAbelha sem ferrãoFenólicosCarotenóidesCompostos voláteis.Propolis is a viscous product produced and collected by worker bees from shots or plant exudates used for the protection of hive. The aims of this work were to perform the phytochemical profile and biological activity of propolis from stingless bee (SB) Melipona quadrifasciata (Mandaçaia) native from Rio Grande do Sul, Brazil. The propolis had a total phenolic concentration of 126,52 mg g-1, and the major phenolic compounds identified were p-coumaric acid (35,64 mg g-1) and gallic acid (18,33 mg g-1). The carotenoids β-carotene, β-criptoxanthin, lutein and lycopene were identified in the propolis of M. quadrifasciata, and β-carotene showed the highest concentration (1.56 mg/100g). The headspace analysis of volatile compounds identified the highest concentration of α-pinene, followed by limonene, sylvestrene and β-pinene. The propolis of M. quadrifasciata showed high antioxidant activity by the DPPH method, with EC50 of 2,29 µg mL-1, antimicrobial activity (MIC) against Escherichia coli and Staphylococcus aureus and low reduction in cell viability of cell lines L929, GL, A549, MDA and SKMEL.El propóleo es un producto viscoso producido y recolectado por abejas obreras, a partir de yemas o exudados de plantas, utilizado para proteger la colmena. El objetivo de este trabajo fue realizar el perfil fitoquímico y la actividad biológica del propóleo de la abeja sin aguijón (ASA) Melipona quadrifasciata (Mandaçaia) originaria de Rio Grande do Sul, Brasil. El propóleo tuvo una concentración fenólica total de 126,52 mg g-1, y los principales compuestos fenólicos identificados fueron ácido p-cumárico (35,64 mg g-1) y ácido gálico (18,33 mg g-1). Los carotenoides β-caroteno, β-criptoxantina, luteína y licopeno fueron identificados en el propóleo de M. quadrifasciata, y el β-caroteno tuvo la mayor concentración (1,56 mg/100g). Los compuestos volátiles se obtuvieron por espacio de cabeza y se identificaron por GC/MS, siendo el α-pineno el compuesto mayoritario, seguido por el limoneno, el silvestreno y el β-pineno. Entre las actividades biológicas realizadas con el propóleo de M. quadrifasciata, mostró alta actividad antirradicalaria por el método DPPH, con EC50 de 2,29 µg mL-1, actividad antimicrobiana (MIC) contra Escherichia coli y Staphylococcus aureus y baja reducción en la viabilidad celular de las líneas celulares L929, GL, A549, MDA y SKMEL.A própolis é um produto viscoso produzido e coletado por abelhas operárias, a partir de brotos ou exsudatos de plantas, utilizados para proteção da colmeia. O objetivo deste trabalho foi realizar o perfil fitoquímico e a atividade biológica da própolis de abelha sem ferrão (ASF) Melipona quadrifasciata (Mandaçaia) nativa do Rio Grande do Sul, Brasil. A própolis apresentou concentração fenólica total de 126,52 mg g-1, e os principais compostos fenólicos identificados foram ácido p-cumárico (35,64 mg g-1) e ácido gálico (18,33 mg g-1). Os carotenóides β-caroteno, β-criptoxantina, luteína e licopeno foram identificados na própolis de M. quadrifasciata, sendo que o β-caroteno apresentou a maior concentração (1,56 mg/100g). Os compostos voláteis foram obtidos por headspace e identificados por CG/EM, sendo o α-pineno o composto majoritário, seguido de limoneno, silvestreno e β-pineno. A própolis apresentou alta atividade antiradicalar pelo método DPPH, com EC50 de 2,29 µg mL-1, atividade antimicrobiana (CIM) contra Escherichia coli e Staphylococcus aureus e baixa redução na viabilidade celular das linhagens celulares L929, GL, A549, MDA e SKMEL.Research, Society and Development2022-09-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3417510.33448/rsd-v11i12.34175Research, Society and Development; Vol. 11 No. 12; e193111234175Research, Society and Development; Vol. 11 Núm. 12; e193111234175Research, Society and Development; v. 11 n. 12; e1931112341752525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/34175/29002Copyright (c) 2022 Alexandre Piccinini; Matheus Henrique Oliveira de Sousa; Marta dos Santos Diniz Freitas; Karina Cesca; Neusa Fernandes de Mourahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessPiccinini, AlexandreSousa, Matheus Henrique Oliveira deFreitas, Marta dos Santos DinizCesca, KarinaMoura, Neusa Fernandes de2022-09-26T11:56:08Zoai:ojs.pkp.sfu.ca:article/34175Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:49:34.846155Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Chemical composition and biological activity of propolis from Melipona quadrifasciata Composición química y actividad biológica del propóleo de Melipona quadrifasciata Composição química e atividade biológica da própolis de Melipona quadrifasciata |
title |
Chemical composition and biological activity of propolis from Melipona quadrifasciata |
spellingShingle |
Chemical composition and biological activity of propolis from Melipona quadrifasciata Piccinini, Alexandre Meliponines Stingless bee Phenolics Carotenoids Volatile compounds. Meliponinas Abeja sin aguijón Fenólicos Carotenoides Compuestos volátiles. Meliponineos Abelha sem ferrão Fenólicos Carotenóides Compostos voláteis. |
title_short |
Chemical composition and biological activity of propolis from Melipona quadrifasciata |
title_full |
Chemical composition and biological activity of propolis from Melipona quadrifasciata |
title_fullStr |
Chemical composition and biological activity of propolis from Melipona quadrifasciata |
title_full_unstemmed |
Chemical composition and biological activity of propolis from Melipona quadrifasciata |
title_sort |
Chemical composition and biological activity of propolis from Melipona quadrifasciata |
author |
Piccinini, Alexandre |
author_facet |
Piccinini, Alexandre Sousa, Matheus Henrique Oliveira de Freitas, Marta dos Santos Diniz Cesca, Karina Moura, Neusa Fernandes de |
author_role |
author |
author2 |
Sousa, Matheus Henrique Oliveira de Freitas, Marta dos Santos Diniz Cesca, Karina Moura, Neusa Fernandes de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Piccinini, Alexandre Sousa, Matheus Henrique Oliveira de Freitas, Marta dos Santos Diniz Cesca, Karina Moura, Neusa Fernandes de |
dc.subject.por.fl_str_mv |
Meliponines Stingless bee Phenolics Carotenoids Volatile compounds. Meliponinas Abeja sin aguijón Fenólicos Carotenoides Compuestos volátiles. Meliponineos Abelha sem ferrão Fenólicos Carotenóides Compostos voláteis. |
topic |
Meliponines Stingless bee Phenolics Carotenoids Volatile compounds. Meliponinas Abeja sin aguijón Fenólicos Carotenoides Compuestos volátiles. Meliponineos Abelha sem ferrão Fenólicos Carotenóides Compostos voláteis. |
description |
Propolis is a viscous product produced and collected by worker bees from shots or plant exudates used for the protection of hive. The aims of this work were to perform the phytochemical profile and biological activity of propolis from stingless bee (SB) Melipona quadrifasciata (Mandaçaia) native from Rio Grande do Sul, Brazil. The propolis had a total phenolic concentration of 126,52 mg g-1, and the major phenolic compounds identified were p-coumaric acid (35,64 mg g-1) and gallic acid (18,33 mg g-1). The carotenoids β-carotene, β-criptoxanthin, lutein and lycopene were identified in the propolis of M. quadrifasciata, and β-carotene showed the highest concentration (1.56 mg/100g). The headspace analysis of volatile compounds identified the highest concentration of α-pinene, followed by limonene, sylvestrene and β-pinene. The propolis of M. quadrifasciata showed high antioxidant activity by the DPPH method, with EC50 of 2,29 µg mL-1, antimicrobial activity (MIC) against Escherichia coli and Staphylococcus aureus and low reduction in cell viability of cell lines L929, GL, A549, MDA and SKMEL. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/34175 10.33448/rsd-v11i12.34175 |
url |
https://rsdjournal.org/index.php/rsd/article/view/34175 |
identifier_str_mv |
10.33448/rsd-v11i12.34175 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/34175/29002 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 12; e193111234175 Research, Society and Development; Vol. 11 Núm. 12; e193111234175 Research, Society and Development; v. 11 n. 12; e193111234175 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052722303729664 |