Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisition

Detalhes bibliográficos
Autor(a) principal: Silva, César Matos Ribeiro da
Data de Publicação: 2021
Outros Autores: Albuquerque , Waleska da Silva, Oliveira , Jucyara Natállia Araujo de, Carneiro , Maria Regina Pires, Jain , Sona, Dolabella , Silvio Santana, Barbosa, Ana Andréa Teixeira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/16178
Resumo: The objective of this study was to analyze the antimicrobial effect of nisin against MRSA (Methicillin-Resistant Staphylococcus aureus) and MSSA (Methicillin-Sensitive Staphylococcus aureus), and at the same time examine the possibility of the bacteria to develope nisin resistance. The antimicrobial susceptibility of the strains was tested using the agar diffusion and/or microdilution methods. To select nisin-resistant strains, bacteria were grown consecutively at sublethal concentrations of the bacteriocin. Nisin showed bactericidal activity against most of the tested strains. MRSA required higher doses of bacteriocin compared to MSSA both for inhibition and cell death. However, transfers in the presence of nisin could completely eliminate nisin activity with an increase in minimal inhibitory concentration value of up to 250 times. Nisin-resistance could be maintained in MRSA and MSSA even in the absence of the bacteriocin. Nisin resistance affected antibiotic susceptibility of both MRSA and MSSA to mainly Cefoxitin, Oxacillin, and Erythromycin. These results indicate that nisin-resistance is a complex trait among MSSA and MRSA and must be elucidated before the therapeutic recommendation of nisin to treat infections caused by these bacterial species.
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spelling Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisitionActividad de nisina contra Staphylococcus aureus resistente y sensible a meticilina y riesgo de adquisición de resistênciaAtividade da nisina contra Staphylococcus aureus resistente e sensível à meticilina e risco de aquisição de resistênciaResistência a antibióticosAtividade antimicrobianaBacteriocinaStaphylococcus aureus.Resistencia a antibióticosActividad antimicrobianaBacteriocinaStaphylococcus aureus.Antibiotic resistanceAntimicrobial activityBacteriocinStaphylococcus aureus.The objective of this study was to analyze the antimicrobial effect of nisin against MRSA (Methicillin-Resistant Staphylococcus aureus) and MSSA (Methicillin-Sensitive Staphylococcus aureus), and at the same time examine the possibility of the bacteria to develope nisin resistance. The antimicrobial susceptibility of the strains was tested using the agar diffusion and/or microdilution methods. To select nisin-resistant strains, bacteria were grown consecutively at sublethal concentrations of the bacteriocin. Nisin showed bactericidal activity against most of the tested strains. MRSA required higher doses of bacteriocin compared to MSSA both for inhibition and cell death. However, transfers in the presence of nisin could completely eliminate nisin activity with an increase in minimal inhibitory concentration value of up to 250 times. Nisin-resistance could be maintained in MRSA and MSSA even in the absence of the bacteriocin. Nisin resistance affected antibiotic susceptibility of both MRSA and MSSA to mainly Cefoxitin, Oxacillin, and Erythromycin. These results indicate that nisin-resistance is a complex trait among MSSA and MRSA and must be elucidated before the therapeutic recommendation of nisin to treat infections caused by these bacterial species.El objetivo de este estudio fue analizar el efecto antimicrobiano de la nisina contra MRSA y MSSA (Staphylococcus aureus resistente a meticilina y sensible a meticilina), y al mismo tiempo examinar la posibilidad de que la bacteria desarrolle resistencia a la nisina. La susceptibilidad antimicrobiana de las cepas se evaluó mediante los métodos de difusión en agar y / o microdilución. Para seleccionar cepas resistentes a la nisina, se cultivaron bacterias consecutivamente a concentraciones subletales de la bacteriocina. La nisina mostró actividad bactericida contra la mayoría de las cepas probadas. MRSA requirió dosis más altas de bacteriocina en comparación con MSSA tanto para la inhibición como para la muerte celular. Sin embargo, las transferencias en presencia de nisina podrían eliminar completamente la actividad de la nisina con un aumento en el valor de MIC de hasta 250 veces. La resistencia a la nisina podría mantenerse en MRSA y MSSA incluso en ausencia de la bacteriocina. La resistencia a la nisina afectó la susceptibilidad antibiótica de MRSA y MSSA principalmente a cefoxitina, oxacilina y eritromicina. Estos resultados indican que la resistencia a la nisina es un rasgo complejo entre MSSA y MRSA y debe aclararse antes de la recomendación terapéutica de la nisina para tratar infecciones causadas por estas especies bacterianas.O objetivo deste estudo foi analisar o efeito antimicrobiano da nisina contra MRSA (Staphylococcus aureus resistente à meticilina) e MSSA (Staphylococcus aureus sensível à meticilina) e, ao mesmo tempo, examinar a possibilidade da bactéria desenvolver resistência à nisina. A susceptibilidade antimicrobiana das cepas foi testada pelos métodos de difusão em ágar e /ou microdiluição. Para selecionar cepas resistentes à nisina, as bactérias foram cultivadas consecutivamente em concentrações subletais da bacteriocina. A nisina apresentou atividade bactericida contra a maioria das cepas testadas. MRSA exigiu doses mais altas de bacteriocina em comparação com MSSA, tanto para inibição quanto para morte celular. No entanto, as transferências na presença de nisina podem eliminar completamente a atividade da nisina com um aumento no valor da concentração mínima inibitória de até 250 vezes. A resistência à nisina pode ser mantida em MRSA e MSSA mesmo na ausência da bacteriocina. A resistência à nisina afetou a suscetibilidade das linhagens principalmente aos antibióticos cefoxitina, oxacilina e eritromicina. Esses resultados indicam que a resistência à nisina é uma característica complexa entre MSSA e MRSA e deve ser elucidada antes da recomendação terapêutica da bacteriocina para o tratamento de infecções causadas por esse microrganismo.Research, Society and Development2021-06-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1617810.33448/rsd-v10i7.16178Research, Society and Development; Vol. 10 No. 7; e4610716178Research, Society and Development; Vol. 10 Núm. 7; e4610716178Research, Society and Development; v. 10 n. 7; e46107161782525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/16178/14518Copyright (c) 2021 César Matos Ribeiro da Silva; Waleska da Silva Albuquerque ; Jucyara Natállia Araujo de Oliveira ; Maria Regina Pires Carneiro ; Sona Jain ; Silvio Santana Dolabella ; Ana Andréa Teixeira Barbosahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, César Matos Ribeiro da Albuquerque , Waleska da Silva Oliveira , Jucyara Natállia Araujo de Carneiro , Maria Regina Pires Jain , SonaDolabella , Silvio Santana Barbosa, Ana Andréa Teixeira 2021-07-18T21:07:03Zoai:ojs.pkp.sfu.ca:article/16178Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:36:48.252827Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisition
Actividad de nisina contra Staphylococcus aureus resistente y sensible a meticilina y riesgo de adquisición de resistência
Atividade da nisina contra Staphylococcus aureus resistente e sensível à meticilina e risco de aquisição de resistência
title Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisition
spellingShingle Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisition
Silva, César Matos Ribeiro da
Resistência a antibióticos
Atividade antimicrobiana
Bacteriocina
Staphylococcus aureus.
Resistencia a antibióticos
Actividad antimicrobiana
Bacteriocina
Staphylococcus aureus.
Antibiotic resistance
Antimicrobial activity
Bacteriocin
Staphylococcus aureus.
title_short Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisition
title_full Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisition
title_fullStr Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisition
title_full_unstemmed Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisition
title_sort Nisin activity against methicillin-resistant and methicillin-sensitive Staphylococcus aureus and risk of resistance acquisition
author Silva, César Matos Ribeiro da
author_facet Silva, César Matos Ribeiro da
Albuquerque , Waleska da Silva
Oliveira , Jucyara Natállia Araujo de
Carneiro , Maria Regina Pires
Jain , Sona
Dolabella , Silvio Santana
Barbosa, Ana Andréa Teixeira
author_role author
author2 Albuquerque , Waleska da Silva
Oliveira , Jucyara Natállia Araujo de
Carneiro , Maria Regina Pires
Jain , Sona
Dolabella , Silvio Santana
Barbosa, Ana Andréa Teixeira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, César Matos Ribeiro da
Albuquerque , Waleska da Silva
Oliveira , Jucyara Natállia Araujo de
Carneiro , Maria Regina Pires
Jain , Sona
Dolabella , Silvio Santana
Barbosa, Ana Andréa Teixeira
dc.subject.por.fl_str_mv Resistência a antibióticos
Atividade antimicrobiana
Bacteriocina
Staphylococcus aureus.
Resistencia a antibióticos
Actividad antimicrobiana
Bacteriocina
Staphylococcus aureus.
Antibiotic resistance
Antimicrobial activity
Bacteriocin
Staphylococcus aureus.
topic Resistência a antibióticos
Atividade antimicrobiana
Bacteriocina
Staphylococcus aureus.
Resistencia a antibióticos
Actividad antimicrobiana
Bacteriocina
Staphylococcus aureus.
Antibiotic resistance
Antimicrobial activity
Bacteriocin
Staphylococcus aureus.
description The objective of this study was to analyze the antimicrobial effect of nisin against MRSA (Methicillin-Resistant Staphylococcus aureus) and MSSA (Methicillin-Sensitive Staphylococcus aureus), and at the same time examine the possibility of the bacteria to develope nisin resistance. The antimicrobial susceptibility of the strains was tested using the agar diffusion and/or microdilution methods. To select nisin-resistant strains, bacteria were grown consecutively at sublethal concentrations of the bacteriocin. Nisin showed bactericidal activity against most of the tested strains. MRSA required higher doses of bacteriocin compared to MSSA both for inhibition and cell death. However, transfers in the presence of nisin could completely eliminate nisin activity with an increase in minimal inhibitory concentration value of up to 250 times. Nisin-resistance could be maintained in MRSA and MSSA even in the absence of the bacteriocin. Nisin resistance affected antibiotic susceptibility of both MRSA and MSSA to mainly Cefoxitin, Oxacillin, and Erythromycin. These results indicate that nisin-resistance is a complex trait among MSSA and MRSA and must be elucidated before the therapeutic recommendation of nisin to treat infections caused by these bacterial species.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/16178
10.33448/rsd-v10i7.16178
url https://rsdjournal.org/index.php/rsd/article/view/16178
identifier_str_mv 10.33448/rsd-v10i7.16178
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/16178/14518
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 7; e4610716178
Research, Society and Development; Vol. 10 Núm. 7; e4610716178
Research, Society and Development; v. 10 n. 7; e4610716178
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052825199443968

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