Chemical characterization, antimicrobial activity and toxicity of essential oils of Pimenta dioica L. and Citrus sinensis L. Osbeck

Detalhes bibliográficos
Autor(a) principal: Everton, Gustavo Oliveira
Data de Publicação: 2020
Outros Autores: Rosa, Paulo Victor Serra, Neves, Silvia Cristina, Pereira, Ana Patricia Matos, Lima, Ericka Cristina Silva, Mendonça, Iago Pereira, Souza, Laurilene dos Santos, Fonseca, Danielly, Cunha, Jean Carlos Rodrigues da, Souza, Lauriane dos Santos, Oliveira, Isaquiel Machado de, Arruda, Mariana Oliveira, Mouchrek Filho, Victor Elias
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/4842
Resumo: This study evaluated the toxicity and antimicrobial activity in the face of Escherichia coli and Staphylococcus aureus of essential oils of Pimenta dioica Lindl. and Citrus sinensis L. The essential oils (EOs) were extracted by hydrodistillation, with chemical characterization by gas chromatography coupled and mass spectrometry (GC-MS). Physicochemical parameters were determined according to the Brazilian Pharmacopeia. The toxicity test followed the bioassay with Artemia salina Leach, the EOs approved in this assay followed to evaluate its biological properties. The antimicrobial activity followed the methodology described by the Clinical and Laboratory Standards Institute using the Disc Diffusion Method, Broth Dilution for Minimum Inhibitory Concentration (MIC) and subsequent minimum bactericide concentration for to evaluate the action of EOs against E. coli and S. aureus. Both EOs showed low toxicity, and thus were evaluated for the biological antimicrobial properties. Both EOs presented bactericidal potential against the microorganisms tested, showing satisfactory results for their action. The results indicate that the evaluated EOs are composed of substances that provide and encourage their application due to their potential for antimicrobial biological activity.
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spelling Chemical characterization, antimicrobial activity and toxicity of essential oils of Pimenta dioica L. and Citrus sinensis L. OsbeckCaracterización química, actividad antimicrobiana y toxicidad de los aceites esenciales de Pimenta dioica L. y Citrus sinensis L. OsbeckCaracterização química, atividade antimicrobiana e toxicidade dos óleos essenciais da Pimenta dioica L. e Citrus sinensis L. OsbeckAceites esencialesActividad antimicrobianaToxicidad.Óleos essenciaisAtividade antimicrobianaToxicidade.Essential oilsActivity antimicrobialToxicity.This study evaluated the toxicity and antimicrobial activity in the face of Escherichia coli and Staphylococcus aureus of essential oils of Pimenta dioica Lindl. and Citrus sinensis L. The essential oils (EOs) were extracted by hydrodistillation, with chemical characterization by gas chromatography coupled and mass spectrometry (GC-MS). Physicochemical parameters were determined according to the Brazilian Pharmacopeia. The toxicity test followed the bioassay with Artemia salina Leach, the EOs approved in this assay followed to evaluate its biological properties. The antimicrobial activity followed the methodology described by the Clinical and Laboratory Standards Institute using the Disc Diffusion Method, Broth Dilution for Minimum Inhibitory Concentration (MIC) and subsequent minimum bactericide concentration for to evaluate the action of EOs against E. coli and S. aureus. Both EOs showed low toxicity, and thus were evaluated for the biological antimicrobial properties. Both EOs presented bactericidal potential against the microorganisms tested, showing satisfactory results for their action. The results indicate that the evaluated EOs are composed of substances that provide and encourage their application due to their potential for antimicrobial biological activity.Este estudio evaluó la toxicidad y la actividad antimicrobiana contra Escherichia coli y Staphylococcus aureus a partir de los aceites esenciales de Pimenta dioica Lindl. y Citrus sinensis L. Los aceites esenciales (AEs) fueron extraídos por hidrodestilación, con caracterización química a través de cromatografía de gases acoplado a espectrometría de masas (GC-MS). Los parámetros fisicoquímicos se determinaron de acuerdo con la Farmacopea Brasileña. El ensayo de toxicidad siguió al bioensayo con Artemia salina Leach, la AE aprobada en este ensayo siguió para evaluar sus propiedades biológicas. La actividad antimicrobiana siguió la metodología descrita por el Instituto de Normas Clínicas y de Laboratorio utilizando el Método de Difusión de Discos, la Dilución de Caldo para la Concentración Mínima Inhibitoria (MIC) y la posterior Concentración Bactericida Mínima para evaluar la acción de las AE contra E. coli y S. aureus. Ambos AEs mostraron baja toxicidad, y por lo tanto fueron evaluados para propiedades biológicas antimicrobianas. Ambos AE presentaron posibles bactericidas contra los microorganismos analizados, mostrando resultados satisfactorios para su acción. Los resultados indican que las AE evaluadas están compuestas de sustancias que proporcionan y fomentan su aplicación debido a su potencial para la actividad biológica antimicrobiana.Este estudo avaliou a toxicidade e a atividade antimicrobiana frente a Escherichia coli e Staphylococcus aureus dos óleos essenciais de Pimenta dioica Lindl. e Citrus sinensis L. Os óleos essenciais (OE’s) foram extraídos por hidrodestilação, com caracterização química através de Cromatografia Gasosa acoplada a espectrometria de massas (CG-EM). Os parâmetros físico-químicos foram determinados de acordo com a Farmacopeia Brasileira. O ensaio de toxicidade seguiu o bioensaio com Artemia salina Leach, os OE’s aprovados neste ensaio seguiram para avaliação das suas propriedades biológicas. A atividade antimicrobiana seguiu a metodologia descrita pelo Clinical and Laboratory Standards Institute utilizando o Método de Difusão de Disco, Diluição em Caldo para Concentração Inibitória Mínima (CIM) e posterior Concentração Bactericida Mínima para avaliar a ação dos OE’s frente a E. coli e S. aureus. Ambos os OE’s apresentaram toxicidade baixa, e assim foram avaliados quanto as propriedades biológicas antimicrobianas. Ambos os OE’s apresentaram potenciais bactericidas frente aos microrganismos testados, exibindo resultados satisfatórios para a ação dos mesmos. Os resultados indicam que os OE’s avaliados são compostos por substâncias que propiciam e incentivam sua aplicação em virtude de seus potenciais para atividade biológica antimicrobianas.Research, Society and Development2020-06-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/484210.33448/rsd-v9i7.4842Research, Society and Development; Vol. 9 No. 7; e803974842Research, Society and Development; Vol. 9 Núm. 7; e803974842Research, Society and Development; v. 9 n. 7; e8039748422525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/4842/4224Copyright (c) 2020 Gustavo Oliveira Everton, Paulo Victor Serra Rosa, Silvia Cristina Neves, Ana Patricia Matos Pereira, Ericka Cristina Silva Lima, Iago Pereira Mendonça, Laurilene dos Santos Souza, Danielly Fonseca, Jean Carlos Rodrigues da Cunha, Lauriane dos Santos Souza, Mariana Oliveira Arruda, Victor Elias Mouchrek Filhoinfo:eu-repo/semantics/openAccessEverton, Gustavo OliveiraRosa, Paulo Victor SerraNeves, Silvia CristinaPereira, Ana Patricia MatosLima, Ericka Cristina SilvaMendonça, Iago PereiraSouza, Laurilene dos SantosFonseca, DaniellyCunha, Jean Carlos Rodrigues daSouza, Lauriane dos SantosOliveira, Isaquiel Machado deArruda, Mariana OliveiraMouchrek Filho, Victor Elias2020-08-20T18:05:03Zoai:ojs.pkp.sfu.ca:article/4842Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:28:33.081884Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Chemical characterization, antimicrobial activity and toxicity of essential oils of Pimenta dioica L. and Citrus sinensis L. Osbeck
Caracterización química, actividad antimicrobiana y toxicidad de los aceites esenciales de Pimenta dioica L. y Citrus sinensis L. Osbeck
Caracterização química, atividade antimicrobiana e toxicidade dos óleos essenciais da Pimenta dioica L. e Citrus sinensis L. Osbeck
title Chemical characterization, antimicrobial activity and toxicity of essential oils of Pimenta dioica L. and Citrus sinensis L. Osbeck
spellingShingle Chemical characterization, antimicrobial activity and toxicity of essential oils of Pimenta dioica L. and Citrus sinensis L. Osbeck
Everton, Gustavo Oliveira
Aceites esenciales
Actividad antimicrobiana
Toxicidad.
Óleos essenciais
Atividade antimicrobiana
Toxicidade.
Essential oils
Activity antimicrobial
Toxicity.
title_short Chemical characterization, antimicrobial activity and toxicity of essential oils of Pimenta dioica L. and Citrus sinensis L. Osbeck
title_full Chemical characterization, antimicrobial activity and toxicity of essential oils of Pimenta dioica L. and Citrus sinensis L. Osbeck
title_fullStr Chemical characterization, antimicrobial activity and toxicity of essential oils of Pimenta dioica L. and Citrus sinensis L. Osbeck
title_full_unstemmed Chemical characterization, antimicrobial activity and toxicity of essential oils of Pimenta dioica L. and Citrus sinensis L. Osbeck
title_sort Chemical characterization, antimicrobial activity and toxicity of essential oils of Pimenta dioica L. and Citrus sinensis L. Osbeck
author Everton, Gustavo Oliveira
author_facet Everton, Gustavo Oliveira
Rosa, Paulo Victor Serra
Neves, Silvia Cristina
Pereira, Ana Patricia Matos
Lima, Ericka Cristina Silva
Mendonça, Iago Pereira
Souza, Laurilene dos Santos
Fonseca, Danielly
Cunha, Jean Carlos Rodrigues da
Souza, Lauriane dos Santos
Oliveira, Isaquiel Machado de
Arruda, Mariana Oliveira
Mouchrek Filho, Victor Elias
author_role author
author2 Rosa, Paulo Victor Serra
Neves, Silvia Cristina
Pereira, Ana Patricia Matos
Lima, Ericka Cristina Silva
Mendonça, Iago Pereira
Souza, Laurilene dos Santos
Fonseca, Danielly
Cunha, Jean Carlos Rodrigues da
Souza, Lauriane dos Santos
Oliveira, Isaquiel Machado de
Arruda, Mariana Oliveira
Mouchrek Filho, Victor Elias
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Everton, Gustavo Oliveira
Rosa, Paulo Victor Serra
Neves, Silvia Cristina
Pereira, Ana Patricia Matos
Lima, Ericka Cristina Silva
Mendonça, Iago Pereira
Souza, Laurilene dos Santos
Fonseca, Danielly
Cunha, Jean Carlos Rodrigues da
Souza, Lauriane dos Santos
Oliveira, Isaquiel Machado de
Arruda, Mariana Oliveira
Mouchrek Filho, Victor Elias
dc.subject.por.fl_str_mv Aceites esenciales
Actividad antimicrobiana
Toxicidad.
Óleos essenciais
Atividade antimicrobiana
Toxicidade.
Essential oils
Activity antimicrobial
Toxicity.
topic Aceites esenciales
Actividad antimicrobiana
Toxicidad.
Óleos essenciais
Atividade antimicrobiana
Toxicidade.
Essential oils
Activity antimicrobial
Toxicity.
description This study evaluated the toxicity and antimicrobial activity in the face of Escherichia coli and Staphylococcus aureus of essential oils of Pimenta dioica Lindl. and Citrus sinensis L. The essential oils (EOs) were extracted by hydrodistillation, with chemical characterization by gas chromatography coupled and mass spectrometry (GC-MS). Physicochemical parameters were determined according to the Brazilian Pharmacopeia. The toxicity test followed the bioassay with Artemia salina Leach, the EOs approved in this assay followed to evaluate its biological properties. The antimicrobial activity followed the methodology described by the Clinical and Laboratory Standards Institute using the Disc Diffusion Method, Broth Dilution for Minimum Inhibitory Concentration (MIC) and subsequent minimum bactericide concentration for to evaluate the action of EOs against E. coli and S. aureus. Both EOs showed low toxicity, and thus were evaluated for the biological antimicrobial properties. Both EOs presented bactericidal potential against the microorganisms tested, showing satisfactory results for their action. The results indicate that the evaluated EOs are composed of substances that provide and encourage their application due to their potential for antimicrobial biological activity.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/4842
10.33448/rsd-v9i7.4842
url https://rsdjournal.org/index.php/rsd/article/view/4842
identifier_str_mv 10.33448/rsd-v9i7.4842
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/4842/4224
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 9 No. 7; e803974842
Research, Society and Development; Vol. 9 Núm. 7; e803974842
Research, Society and Development; v. 9 n. 7; e803974842
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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