Evaluation of the antibacterial and antifungal potential of organic hydrolate and organic essential oil from Lavandula dentata L. (Lamiaceae)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/36076 |
Resumo: | The Lavandula dentata L. (Lamiaceae) is used by Mediterranean peoples for its numerous applications. Its secondary pathways operate producing a wide variety of volatile terpenoids that act as efficient antifungals and bactericides. Therefore, this research aimed to evaluate the antimicrobial potential of the organic hydrolate and organic essential oil of L. dentata against bacterial strains of Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538 and ATCC 25923), Streptococcus pyogenes (ATCC 19615) and Mycobacterium tuberculosis (ATCC 25177), yeast strains of Candida albicans (ATCC 10231), Candida parapsilosis (ATCC 22019), Cryptococcus neoformans var. grubii. (ATCC 90113) and the filamentous fungus Fusarium oxysporum f. sp. cubense (CMAA 1807) by disk diffusion method. The disk diffusion test was performed in Mueller Hinton Agar medium, with 2% glucose supplementation for the yeasts. As a positive control, 70% ethyl alcohol was adopted, the antibiotic cephalexin (5 ug) and the antifungal fluconazole (25 ug) were chosen to evaluate the sensitivity profile. The statistical test of variance ANOVA was adopted to determine the significance of the results obtained. The essential oil was efficient against the microorganisms evaluated, especially against the gram-positive strains of Staphylococcus aureus and Mycobacterium tuberculosis. It also showed a higher inhibitory potential to ethyl alcohol against Candida albicans and Streptococcus pyogenes. Therefore, it is inferred that the essential oil of Lavandula dentata has high antimicrobial capacity against gram-positive strains, presenting itself as a potential antimicrobial agent against microbial strains of considerable importance. |
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Evaluation of the antibacterial and antifungal potential of organic hydrolate and organic essential oil from Lavandula dentata L. (Lamiaceae)Evaluación del potencial antibacteriano y antifúngico del hidrolato orgánico y aceite esencial orgánico de Lavandula dentata L. (Lamiaceae)Avaliação do potencial antibacteriano e antifúngico do hidrolato e óleo essencial orgânicos de Lavandula dentata L. (Lamiaceae)AntimicrobialBacteriaLavenderYeastFungus.AntimicrobianoBacteriasLavandaLevaduraHongos.AntimicrobianoBactériasLavandaLevedurasFungos.The Lavandula dentata L. (Lamiaceae) is used by Mediterranean peoples for its numerous applications. Its secondary pathways operate producing a wide variety of volatile terpenoids that act as efficient antifungals and bactericides. Therefore, this research aimed to evaluate the antimicrobial potential of the organic hydrolate and organic essential oil of L. dentata against bacterial strains of Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538 and ATCC 25923), Streptococcus pyogenes (ATCC 19615) and Mycobacterium tuberculosis (ATCC 25177), yeast strains of Candida albicans (ATCC 10231), Candida parapsilosis (ATCC 22019), Cryptococcus neoformans var. grubii. (ATCC 90113) and the filamentous fungus Fusarium oxysporum f. sp. cubense (CMAA 1807) by disk diffusion method. The disk diffusion test was performed in Mueller Hinton Agar medium, with 2% glucose supplementation for the yeasts. As a positive control, 70% ethyl alcohol was adopted, the antibiotic cephalexin (5 ug) and the antifungal fluconazole (25 ug) were chosen to evaluate the sensitivity profile. The statistical test of variance ANOVA was adopted to determine the significance of the results obtained. The essential oil was efficient against the microorganisms evaluated, especially against the gram-positive strains of Staphylococcus aureus and Mycobacterium tuberculosis. It also showed a higher inhibitory potential to ethyl alcohol against Candida albicans and Streptococcus pyogenes. Therefore, it is inferred that the essential oil of Lavandula dentata has high antimicrobial capacity against gram-positive strains, presenting itself as a potential antimicrobial agent against microbial strains of considerable importance.La Lavandula dentata L. (Lamiaceae) es utilizada por los pueblos mediterráneos por sus numerosas aplicaciones. Sus rutas secundarias operan produciendo una amplia variedad de terpenoides volátiles que actúan como eficientes antifúngicos y bactericidas. Por lo tanto, esta investigación tuvo como objetivo evaluar el potencial antimicrobiano del hidrolato orgánico y aceite esencial orgánico de L. dentata contra cepas bacterianas de Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538 y ATCC 25923), Streptococcus pyogenes (ATCC 19615) y Mycobacterium tuberculosis (ATCC 25177), cepas de levadura de Candida albicans (ATCC 10231), Candida parapsilosis (ATCC 22019), Cryptococcus neoformans var. grubii. (ATCC 90113) y el hongo filamentoso Fusarium oxysporum f. sp. cubense (CMAA 1807) utilizando el método de difusión con discos. La prueba de difusión con disco se realizó en medio Mueller Hinton Agar, con suplemento de glucosa al 2% para las levaduras. Como control positivo se adoptó alcohol etílico al 70%, se eligió el antibiótico cefalexina (5 ug) y el antifúngico fluconazol (25 ug) para evaluar el perfil de sensibilidad. Se adoptó la prueba estadística de varianza ANOVA para determinar la significación de los resultados obtenidos. El aceite esencial fue eficaz contra los microorganismos evaluados, especialmente contra las cepas grampositivas de Staphylococcus aureus y Mycobacterium tuberculosis. También mostró un potencial inhibitorio superior al alcohol etílico contra Candida albicans y Streptococcus pyogenes. Por lo tanto, se infiere que el aceite esencial de Lavandula dentata tiene alta capacidad antimicrobiana frente a cepas grampositivas, presentándose como un potencial agente antimicrobiano frente a cepas microbianas de considerable importancia.A Lavandula dentata L. (Lamiaceae) é utilizada por povos mediterrânicos pelas suas inúmeras aplicações na medicina tradicional. Suas vias secundárias operam produzindo grande variedade de terpenoides voláteis que atuam como eficientes antifúngicos e bactericidas. Logo, objetivou-se nesta pesquisa avaliar o potencial antimicrobiano do hidrolato e óleo essencial orgânicos de L. dentata, frente estirpes bacterianas de Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538 e ATCC 25923), Streptococcus pyogenes (ATCC 19615) e Mycobacterium tuberculosis (ATCC 25177), estirpes leveduriformes de Candida albicans (ATCC 10231), Candida parapsilosis (ATCC 22019), Cryptococcus neoformans var. grubii. (ATCC 90113) e o fungo filamentoso Fusarium oxysporum f. sp. cubense (CMAA 1807) pela técnica de disco-difusão em meio. O teste de disco-difusão foi realizado em meio Ágar Mueller Hinton, com suplementação de 2% de glicose para as leveduras. Como controle positivo adotou-se o álcool etílico 70%, o antibiótico cefalexina (5 ug) e o antifúngico fluconazol (25 ug) foram escolhidos para avaliação do perfil de sensibilidade. Adotou-se o teste estatístico de variância ANOVA para determinação da significância dos resultados obtidos. O óleo essencial foi eficiente frente os microrganismos avaliados, especialmente frente as cepas gram-positivas de Staphylococcus aureus e Mycobacterium tuberculosis. Este, ainda, apresentou potencial inibitório superior ao álcool etílico frente Candida albicans e Streptococcus pyogenes. Portanto, infere-se que o óleo essencial de Lavandula dentata detém alta capacidade antimicrobiana frente estirpes gram-positivas, apresentando-se como um potencial agente antimicrobiano frente cepas microbianas de considerável importância.Research, Society and Development2022-10-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3607610.33448/rsd-v11i14.36076Research, Society and Development; Vol. 11 No. 14; e95111436076Research, Society and Development; Vol. 11 Núm. 14; e95111436076Research, Society and Development; v. 11 n. 14; e951114360762525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/36076/30153Copyright (c) 2022 Túlio Custódio Reis; Mateus Carvalho Pereira; Carolina Passarelli Gonçalves; Francisco Eduardo de Carvalho Costahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessReis, Túlio Custódio Pereira, Mateus Carvalho Gonçalves, Carolina Passarelli Costa, Francisco Eduardo de Carvalho 2022-11-08T13:36:27Zoai:ojs.pkp.sfu.ca:article/36076Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:50:45.562670Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Evaluation of the antibacterial and antifungal potential of organic hydrolate and organic essential oil from Lavandula dentata L. (Lamiaceae) Evaluación del potencial antibacteriano y antifúngico del hidrolato orgánico y aceite esencial orgánico de Lavandula dentata L. (Lamiaceae) Avaliação do potencial antibacteriano e antifúngico do hidrolato e óleo essencial orgânicos de Lavandula dentata L. (Lamiaceae) |
title |
Evaluation of the antibacterial and antifungal potential of organic hydrolate and organic essential oil from Lavandula dentata L. (Lamiaceae) |
spellingShingle |
Evaluation of the antibacterial and antifungal potential of organic hydrolate and organic essential oil from Lavandula dentata L. (Lamiaceae) Reis, Túlio Custódio Antimicrobial Bacteria Lavender Yeast Fungus. Antimicrobiano Bacterias Lavanda Levadura Hongos. Antimicrobiano Bactérias Lavanda Leveduras Fungos. |
title_short |
Evaluation of the antibacterial and antifungal potential of organic hydrolate and organic essential oil from Lavandula dentata L. (Lamiaceae) |
title_full |
Evaluation of the antibacterial and antifungal potential of organic hydrolate and organic essential oil from Lavandula dentata L. (Lamiaceae) |
title_fullStr |
Evaluation of the antibacterial and antifungal potential of organic hydrolate and organic essential oil from Lavandula dentata L. (Lamiaceae) |
title_full_unstemmed |
Evaluation of the antibacterial and antifungal potential of organic hydrolate and organic essential oil from Lavandula dentata L. (Lamiaceae) |
title_sort |
Evaluation of the antibacterial and antifungal potential of organic hydrolate and organic essential oil from Lavandula dentata L. (Lamiaceae) |
author |
Reis, Túlio Custódio |
author_facet |
Reis, Túlio Custódio Pereira, Mateus Carvalho Gonçalves, Carolina Passarelli Costa, Francisco Eduardo de Carvalho |
author_role |
author |
author2 |
Pereira, Mateus Carvalho Gonçalves, Carolina Passarelli Costa, Francisco Eduardo de Carvalho |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Reis, Túlio Custódio Pereira, Mateus Carvalho Gonçalves, Carolina Passarelli Costa, Francisco Eduardo de Carvalho |
dc.subject.por.fl_str_mv |
Antimicrobial Bacteria Lavender Yeast Fungus. Antimicrobiano Bacterias Lavanda Levadura Hongos. Antimicrobiano Bactérias Lavanda Leveduras Fungos. |
topic |
Antimicrobial Bacteria Lavender Yeast Fungus. Antimicrobiano Bacterias Lavanda Levadura Hongos. Antimicrobiano Bactérias Lavanda Leveduras Fungos. |
description |
The Lavandula dentata L. (Lamiaceae) is used by Mediterranean peoples for its numerous applications. Its secondary pathways operate producing a wide variety of volatile terpenoids that act as efficient antifungals and bactericides. Therefore, this research aimed to evaluate the antimicrobial potential of the organic hydrolate and organic essential oil of L. dentata against bacterial strains of Escherichia coli (ATCC 8739), Staphylococcus aureus (ATCC 6538 and ATCC 25923), Streptococcus pyogenes (ATCC 19615) and Mycobacterium tuberculosis (ATCC 25177), yeast strains of Candida albicans (ATCC 10231), Candida parapsilosis (ATCC 22019), Cryptococcus neoformans var. grubii. (ATCC 90113) and the filamentous fungus Fusarium oxysporum f. sp. cubense (CMAA 1807) by disk diffusion method. The disk diffusion test was performed in Mueller Hinton Agar medium, with 2% glucose supplementation for the yeasts. As a positive control, 70% ethyl alcohol was adopted, the antibiotic cephalexin (5 ug) and the antifungal fluconazole (25 ug) were chosen to evaluate the sensitivity profile. The statistical test of variance ANOVA was adopted to determine the significance of the results obtained. The essential oil was efficient against the microorganisms evaluated, especially against the gram-positive strains of Staphylococcus aureus and Mycobacterium tuberculosis. It also showed a higher inhibitory potential to ethyl alcohol against Candida albicans and Streptococcus pyogenes. Therefore, it is inferred that the essential oil of Lavandula dentata has high antimicrobial capacity against gram-positive strains, presenting itself as a potential antimicrobial agent against microbial strains of considerable importance. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/36076 10.33448/rsd-v11i14.36076 |
url |
https://rsdjournal.org/index.php/rsd/article/view/36076 |
identifier_str_mv |
10.33448/rsd-v11i14.36076 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/36076/30153 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 14; e95111436076 Research, Society and Development; Vol. 11 Núm. 14; e95111436076 Research, Society and Development; v. 11 n. 14; e95111436076 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052726450847744 |