Evaluation of the toxicity of different concentrations of mallow and marigold tinctures through the bioassay with Artemia salina

Detalhes bibliográficos
Autor(a) principal: Tchemra, Flávia Gouvêa Costa
Data de Publicação: 2022
Outros Autores: Pontes, Mateus Mota, Melo, Aline Rocha de, Geus, Juliana Larocca de, Kozlowski Júnior, Vitoldo Antônio, Rezende, Márcia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/26255
Resumo: The objective was to evaluate the toxicity of tinctures of mallow 10% (Malva sylvestris) and marigold 10% (Calendula officinalis), through the bioassay with Artemia salina. The nauplii were obtained and transferred to test tubes, which contained mallow and marigold tinctures in different concentrations (25, 50, 75, 150, 250, 500, 750 and 1000 μg/ml), and the control substances (saline water and 0.12% chlorhexidine. The test was performed in triplicate and live nauplii were counted after 24 h. The mean lethal concentration (LC50) was obtained and submitted to Probit analysis using the StatPlus® software. Survival curves and LogRank tests were performed for the tinctures compared to controls, using the GraphPad® Prism software. The LC50 (μg/ml ± standard deviation) for mallow was 276.08 ± 41.12, and for marigold 268.95 ± 44.09, both tinctures being considered active. There was a statistical difference in the survival curve for both mallow and marigold when compared to saline solution (p<0.0001), indicating greater toxicity of the tinctures. There was also a statistical difference when the tinctures were compared to chlorhexidine (p<0.0001), indicating greater toxicity of chlorhexidine. There was no difference in the survival rate of nauplii, when the mallow and marigold tinctures were analyzed (p = 0.8089). It was concluded that the tinctures of mallow 10% and marigold 10% presented moderate toxicity against Artemia salina.
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spelling Evaluation of the toxicity of different concentrations of mallow and marigold tinctures through the bioassay with Artemia salina Evaluación de la toxicidad de diferentes concentraciones de tinturas de malva y caléndula mediante el bioensayo con Artemia salinaAvaliação da toxicidade de diferentes concentrações de tinturas de malva e calêndula através do bioensaio com Artemia salina MalvaCalendulaPhytotherapyToxicity tests, acute.MalvaCalendulaFitoterapiaPruebas de toxicidad aguda.MalvaCalendulaFitoterapiaTestes de toxicidade aguda.The objective was to evaluate the toxicity of tinctures of mallow 10% (Malva sylvestris) and marigold 10% (Calendula officinalis), through the bioassay with Artemia salina. The nauplii were obtained and transferred to test tubes, which contained mallow and marigold tinctures in different concentrations (25, 50, 75, 150, 250, 500, 750 and 1000 μg/ml), and the control substances (saline water and 0.12% chlorhexidine. The test was performed in triplicate and live nauplii were counted after 24 h. The mean lethal concentration (LC50) was obtained and submitted to Probit analysis using the StatPlus® software. Survival curves and LogRank tests were performed for the tinctures compared to controls, using the GraphPad® Prism software. The LC50 (μg/ml ± standard deviation) for mallow was 276.08 ± 41.12, and for marigold 268.95 ± 44.09, both tinctures being considered active. There was a statistical difference in the survival curve for both mallow and marigold when compared to saline solution (p<0.0001), indicating greater toxicity of the tinctures. There was also a statistical difference when the tinctures were compared to chlorhexidine (p<0.0001), indicating greater toxicity of chlorhexidine. There was no difference in the survival rate of nauplii, when the mallow and marigold tinctures were analyzed (p = 0.8089). It was concluded that the tinctures of mallow 10% and marigold 10% presented moderate toxicity against Artemia salina.El objetivo fue evaluar la toxicidad de las tinturas de malva 10% (Malva sylvestris) y caléndula 10% (Calendula officinalis), mediante el bioensayo con Artemia salina. Los nauplios se obtuvieron y transfirieron a tubos de ensayo, los cuales contenían tinturas de malva y caléndula en diferentes concentraciones (25, 50, 75, 150, 250, 500, 750 y 1000 μg / ml), y las sustancias de control (agua salina y 0.12% clorhexidina. La prueba se realizó por triplicado y se contaron los nauplios vivos después de 24 h. La concentración letal media (CL50) se obtuvo y se sometió a análisis Probit utilizando el software StatPlus®. Se realizaron curvas de supervivencia y pruebas de LogRank para los tintes en comparación con los controles, utilizando el software GraphPad® Prism. La CL50 (μg/ml ± desviación estándar) para malva fue 276,08 ± 41,12 y para caléndula 268,95 ± 44,09, considerándose ambos colorantes activos. Hubo una diferencia estadística en la curva de supervivencia tanto para la malva como para la caléndula en comparación con la solución salina (p <0,0001), lo que indica una mayor toxicidad de las tinturas. También hubo una diferencia estadística cuando se compararon los tintes con la clorhexidina (p <0,0001), lo que indica una mayor toxicidad de la clorhexidina. No hubo diferencia en la tasa de supervivencia de nauplios, cuando se analizaron las tinturas de malva y caléndula (p = 0,8089). Se concluyó que las tinturas de malva 10% y caléndula 10% presentaron toxicidad moderada frente a Artemia salina.O objetivo foi avaliar a toxicidade das tinturas de malva 10% (Malva sylvestris) e calêndula 10% (Calendula officinalis), através do bioensaio com Artemia salina. Os náuplios foram obtidos e transferidos para tubos de ensaio, os quais continham tinturas de malva e calêndula em diferentes concentrações (25, 50, 75, 150, 250, 500, 750 e 1000 μg/ml), e substâncias controle (água salina e clorexidina 0,12%). O teste foi realizado em triplicata e os náuplios vivos foram contados após 24 h. A concentração letal média (CL50) foi obtida e submetida a análise Probit através do software StatPlus®. As curvas de sobrevivência e testes de LogRank foram realizados para as tinturas comparando com os controles, através do software GraphPad® Prism. A CL50 (μg/ml ± erro-padrão) para a malva foi de 276,08 ± 41,12, e para a calêndula 268,95 ± 44,09, sendo ambas as tinturas consideradas ativas. Verificou-se diferença estatística na curva de sobrevivência tanto para a malva quanto para a calêndula quando comparadas com solução salina (p<0,0001), indicando maior toxicidade das tinturas. Também houve diferença estatística quando as tinturas foram comparadas a clorexidina (p<0,0001), indicando maior toxicidade da clorexidina. Não houve diferença na taxa de sobrevivência dos náuplios, quando analisadas as tinturas de malva e calêndula (p = 0,8089). Pôde-se concluir que as tinturas de malva 10% e calêndula 10% apresentaram toxicidade moderada frente a Artemia salina.Research, Society and Development2022-02-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2625510.33448/rsd-v11i3.26255Research, Society and Development; Vol. 11 No. 3; e20511326255Research, Society and Development; Vol. 11 Núm. 3; e20511326255Research, Society and Development; v. 11 n. 3; e205113262552525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/26255/23157Copyright (c) 2022 Flávia Gouvêa Costa Tchemra; Mateus Mota Pontes; Aline Rocha de Melo; Juliana Larocca de Geus; Vitoldo Antônio Kozlowski Júnior; Márcia Rezendehttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessTchemra, Flávia Gouvêa CostaPontes, Mateus MotaMelo, Aline Rocha de Geus, Juliana Larocca de Kozlowski Júnior, Vitoldo AntônioRezende, Márcia2022-03-09T13:44:38Zoai:ojs.pkp.sfu.ca:article/26255Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:44:20.570344Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Evaluation of the toxicity of different concentrations of mallow and marigold tinctures through the bioassay with Artemia salina
Evaluación de la toxicidad de diferentes concentraciones de tinturas de malva y caléndula mediante el bioensayo con Artemia salina
Avaliação da toxicidade de diferentes concentrações de tinturas de malva e calêndula através do bioensaio com Artemia salina
title Evaluation of the toxicity of different concentrations of mallow and marigold tinctures through the bioassay with Artemia salina
spellingShingle Evaluation of the toxicity of different concentrations of mallow and marigold tinctures through the bioassay with Artemia salina
Tchemra, Flávia Gouvêa Costa
Malva
Calendula
Phytotherapy
Toxicity tests, acute.
Malva
Calendula
Fitoterapia
Pruebas de toxicidad aguda.
Malva
Calendula
Fitoterapia
Testes de toxicidade aguda.
title_short Evaluation of the toxicity of different concentrations of mallow and marigold tinctures through the bioassay with Artemia salina
title_full Evaluation of the toxicity of different concentrations of mallow and marigold tinctures through the bioassay with Artemia salina
title_fullStr Evaluation of the toxicity of different concentrations of mallow and marigold tinctures through the bioassay with Artemia salina
title_full_unstemmed Evaluation of the toxicity of different concentrations of mallow and marigold tinctures through the bioassay with Artemia salina
title_sort Evaluation of the toxicity of different concentrations of mallow and marigold tinctures through the bioassay with Artemia salina
author Tchemra, Flávia Gouvêa Costa
author_facet Tchemra, Flávia Gouvêa Costa
Pontes, Mateus Mota
Melo, Aline Rocha de
Geus, Juliana Larocca de
Kozlowski Júnior, Vitoldo Antônio
Rezende, Márcia
author_role author
author2 Pontes, Mateus Mota
Melo, Aline Rocha de
Geus, Juliana Larocca de
Kozlowski Júnior, Vitoldo Antônio
Rezende, Márcia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Tchemra, Flávia Gouvêa Costa
Pontes, Mateus Mota
Melo, Aline Rocha de
Geus, Juliana Larocca de
Kozlowski Júnior, Vitoldo Antônio
Rezende, Márcia
dc.subject.por.fl_str_mv Malva
Calendula
Phytotherapy
Toxicity tests, acute.
Malva
Calendula
Fitoterapia
Pruebas de toxicidad aguda.
Malva
Calendula
Fitoterapia
Testes de toxicidade aguda.
topic Malva
Calendula
Phytotherapy
Toxicity tests, acute.
Malva
Calendula
Fitoterapia
Pruebas de toxicidad aguda.
Malva
Calendula
Fitoterapia
Testes de toxicidade aguda.
description The objective was to evaluate the toxicity of tinctures of mallow 10% (Malva sylvestris) and marigold 10% (Calendula officinalis), through the bioassay with Artemia salina. The nauplii were obtained and transferred to test tubes, which contained mallow and marigold tinctures in different concentrations (25, 50, 75, 150, 250, 500, 750 and 1000 μg/ml), and the control substances (saline water and 0.12% chlorhexidine. The test was performed in triplicate and live nauplii were counted after 24 h. The mean lethal concentration (LC50) was obtained and submitted to Probit analysis using the StatPlus® software. Survival curves and LogRank tests were performed for the tinctures compared to controls, using the GraphPad® Prism software. The LC50 (μg/ml ± standard deviation) for mallow was 276.08 ± 41.12, and for marigold 268.95 ± 44.09, both tinctures being considered active. There was a statistical difference in the survival curve for both mallow and marigold when compared to saline solution (p<0.0001), indicating greater toxicity of the tinctures. There was also a statistical difference when the tinctures were compared to chlorhexidine (p<0.0001), indicating greater toxicity of chlorhexidine. There was no difference in the survival rate of nauplii, when the mallow and marigold tinctures were analyzed (p = 0.8089). It was concluded that the tinctures of mallow 10% and marigold 10% presented moderate toxicity against Artemia salina.
publishDate 2022
dc.date.none.fl_str_mv 2022-02-19
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/26255
10.33448/rsd-v11i3.26255
url https://rsdjournal.org/index.php/rsd/article/view/26255
identifier_str_mv 10.33448/rsd-v11i3.26255
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/26255/23157
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 3; e20511326255
Research, Society and Development; Vol. 11 Núm. 3; e20511326255
Research, Society and Development; v. 11 n. 3; e20511326255
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052704678215680

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