The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/14765 |
Resumo: | To evaluate the influence of reciprocating single-file instrumentation with different working lengths (WL) on the reduction of planktonic bacteria and bacterial biofilm in Enterococcus faecalis-contaminated oval root canals. Methodology: Fifty-five human single-rooted canines were used. Fifty were inoculated with E. faecalis for 21 days for biofilm formation. To confirm the formation of biofilm adhered to the root canal wall, 5 contaminated samples from positive control group were analyzed by SEM. Samples were assigned into 3 groups (n = 15) according to working length determined, G+1 root canal preparation 1 mm beyond the apical foramen, G0 root canal preparation at the major foramen, and G-1 root canal preparation 1 mm short of the major foramen. Five roots were not inoculated to serve as a negative control. Bacteriological samples were collected prior to preparation, initial collection (S1), and after reciprocating instrumentation (S2) by disaggregating biofilm to quantify the reduction of planktonic bacteria and intracanal biofilm at different WL. Bacterial quantitation was performed using colony-forming units per milliliter (CFU / mL) count. Statistical analysis was performed at the significance level of 0.05. Results: No bacterial growth was observed in the negative control. All positive controls demonstrated bacterial growth; S1 from all teeth were positive for bacteria with no significant difference. The post-hoc analysis showed G+1 promoting a significantly higher disinfection than G-1 (p<0,05) and G-1 similar disinfection to G0 (P=962). Conclusion: Instrumentation as close as possible to major foramen or beyond it improves decontamination in oval root canals with reciprocating instrumentation. |
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The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentationLa influencia de la longitud de trabajo en la reducción de biopelículas y bacterias plantónicas en canales ovalados con instrumentación recíprocaA influência do comprimento de trabalho na redução de biofilme e bactérias plantônicas em canais ovais com instrumentação reciprocanteEnterococcus faecalisForame apicalDesinfecçãoTratamento do canal radicular.Enterococcus faecalisApical foramenDisinfectionRoot canal therapy.Enterococcus faecalisForamen apicalDesinfecciónTratamiento de conducto.To evaluate the influence of reciprocating single-file instrumentation with different working lengths (WL) on the reduction of planktonic bacteria and bacterial biofilm in Enterococcus faecalis-contaminated oval root canals. Methodology: Fifty-five human single-rooted canines were used. Fifty were inoculated with E. faecalis for 21 days for biofilm formation. To confirm the formation of biofilm adhered to the root canal wall, 5 contaminated samples from positive control group were analyzed by SEM. Samples were assigned into 3 groups (n = 15) according to working length determined, G+1 root canal preparation 1 mm beyond the apical foramen, G0 root canal preparation at the major foramen, and G-1 root canal preparation 1 mm short of the major foramen. Five roots were not inoculated to serve as a negative control. Bacteriological samples were collected prior to preparation, initial collection (S1), and after reciprocating instrumentation (S2) by disaggregating biofilm to quantify the reduction of planktonic bacteria and intracanal biofilm at different WL. Bacterial quantitation was performed using colony-forming units per milliliter (CFU / mL) count. Statistical analysis was performed at the significance level of 0.05. Results: No bacterial growth was observed in the negative control. All positive controls demonstrated bacterial growth; S1 from all teeth were positive for bacteria with no significant difference. The post-hoc analysis showed G+1 promoting a significantly higher disinfection than G-1 (p<0,05) and G-1 similar disinfection to G0 (P=962). Conclusion: Instrumentation as close as possible to major foramen or beyond it improves decontamination in oval root canals with reciprocating instrumentation.Evaluar la influencia de la instrumentación recíproca con diferentes longitudes de trabajo (LT) en la reducción de bacterias planctónicas y biofilm bacteriano en conductos ovalados contaminados con Enterococcus faecalis. Metodología: Se utilizaron 55 caninos humanos; 50 se inocularon con E. faecalis durante 21 días para la formación de biopelículas. Para confirmar la formación de biopelícula se analizaron por SEM 5 muestras contaminadas del grupo de control positivo. Las muestras se asignaron en 3 grupos (n = 15) de acuerdo con la LT determinada, preparación del conducto radicular G + 1 1 mm más allá del foramen apical, preparación del conducto radicular G0 en el foramen mayor y preparación del conducto radicular G-1 1 mm antes de la el foramen mayor. No se inocularon cinco raíces (control negativo). Se recolectaron muestras bacteriológicas antes de la preparación (S1) y después de la instrumentación recíproca (S2) para cuantificar la reducción de bacterias planctónicas y biofilm intracanal en diferentes WL. La cuantificación bacteriana se realizó utilizando unidades formadoras de colonias por mililitro (CFU / mL) de recuento. El análisis estadístico se realizó al nivel de significancia de 0.05. Resultados: No se observó crecimiento bacteriano en el control negativo. Todos los controles positivos demostraron crecimiento bacteriano; El S1 de todos los dientes fue positivo para bacterias sin diferencias significativas. El análisis post-hoc mostró que G + 1 promueve una desinfección mayor que G-1 (p <0,05) y G-1 una desinfección similar a G0 (P = 962). Conclusión: La instrumentación lo más cerca posible del foramen mayor o más allá de este mejora la descontaminación en los conductos radiculares con instrumentación recíproca.Avaliar a influência da instrumentação reciprocante com diferentes comprimentos de trabalho (CT) na redução de bactérias planctônicas e biofilme bacteriano em canais ovais contaminados com Enterococcus faecalis. Metodologia: Foram utilizados 55 caninos humanos unirradiculares. Cinqüenta foram inoculados com E. faecalis por 21 dias para a formação de biofilme. Para confirmar a formação de biofilme, 5 amostras contaminadas do grupo controle positivo foram analisadas por MEV. As amostras foram divididas em 3 grupos (n = 15) de acordo com o CT, preparo do canal radicular G + 1, 1 mm além do forame apical, preparo do canal radicular G0 no forame principal e preparo do canal radicular G-1m 1 mm antes de o forame principal. Cinco raízes não foram inoculadas para (controle negativo). Amostras bacteriológicas foram coletadas antes do preparo (S1) e após instrumentação (S2) quantificar a redução de bactérias planctônicas e biofilme intracanal em diferentes WL. A quantificação bacteriana foi realizada usando unidades formadoras de colônias por mililitro (UFC / mL) contagem. A análise estatística foi realizada com significância de 0,05. Resultados: Nenhum crescimento bacteriano foi observado no controle negativo. Todos os controles positivos demonstraram crescimento bacteriano; S1 de todos os dentes foram positivos para bactérias sem diferença significativa. A análise post-hoc mostrou que o G + 1 promoveu uma desinfecção maior do que o G-1 (p <0,05) e o G-1 desinfecção semelhante ao G0 (P = 962). Conclusão: A instrumentação o mais próximo possível do forame principal ou além melhora a descontaminação em canais ovais com instrumentação reciprocante.Research, Society and Development2021-04-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1476510.33448/rsd-v10i5.14765Research, Society and Development; Vol. 10 No. 5; e9810514765Research, Society and Development; Vol. 10 Núm. 5; e9810514765Research, Society and Development; v. 10 n. 5; e98105147652525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/14765/13195Copyright (c) 2021 Arieth Cristina Sacomani; Fernanda Cintra; Adriana de Jesus Soares; Marcos Frozonihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSacomani, Arieth Cristina Cintra, Fernanda TessaroSoares, Adriana de JesusFrozoni, Marcos2021-05-17T18:20:49Zoai:ojs.pkp.sfu.ca:article/14765Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:35:47.312751Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation La influencia de la longitud de trabajo en la reducción de biopelículas y bacterias plantónicas en canales ovalados con instrumentación recíproca A influência do comprimento de trabalho na redução de biofilme e bactérias plantônicas em canais ovais com instrumentação reciprocante |
title |
The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation |
spellingShingle |
The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation Sacomani, Arieth Cristina Enterococcus faecalis Forame apical Desinfecção Tratamento do canal radicular. Enterococcus faecalis Apical foramen Disinfection Root canal therapy. Enterococcus faecalis Foramen apical Desinfección Tratamiento de conducto. |
title_short |
The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation |
title_full |
The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation |
title_fullStr |
The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation |
title_full_unstemmed |
The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation |
title_sort |
The influence of working length on the reduction of biofilm and planktonic bacteria in oval canals with reciprocating instrumentation |
author |
Sacomani, Arieth Cristina |
author_facet |
Sacomani, Arieth Cristina Cintra, Fernanda Tessaro Soares, Adriana de Jesus Frozoni, Marcos |
author_role |
author |
author2 |
Cintra, Fernanda Tessaro Soares, Adriana de Jesus Frozoni, Marcos |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Sacomani, Arieth Cristina Cintra, Fernanda Tessaro Soares, Adriana de Jesus Frozoni, Marcos |
dc.subject.por.fl_str_mv |
Enterococcus faecalis Forame apical Desinfecção Tratamento do canal radicular. Enterococcus faecalis Apical foramen Disinfection Root canal therapy. Enterococcus faecalis Foramen apical Desinfección Tratamiento de conducto. |
topic |
Enterococcus faecalis Forame apical Desinfecção Tratamento do canal radicular. Enterococcus faecalis Apical foramen Disinfection Root canal therapy. Enterococcus faecalis Foramen apical Desinfección Tratamiento de conducto. |
description |
To evaluate the influence of reciprocating single-file instrumentation with different working lengths (WL) on the reduction of planktonic bacteria and bacterial biofilm in Enterococcus faecalis-contaminated oval root canals. Methodology: Fifty-five human single-rooted canines were used. Fifty were inoculated with E. faecalis for 21 days for biofilm formation. To confirm the formation of biofilm adhered to the root canal wall, 5 contaminated samples from positive control group were analyzed by SEM. Samples were assigned into 3 groups (n = 15) according to working length determined, G+1 root canal preparation 1 mm beyond the apical foramen, G0 root canal preparation at the major foramen, and G-1 root canal preparation 1 mm short of the major foramen. Five roots were not inoculated to serve as a negative control. Bacteriological samples were collected prior to preparation, initial collection (S1), and after reciprocating instrumentation (S2) by disaggregating biofilm to quantify the reduction of planktonic bacteria and intracanal biofilm at different WL. Bacterial quantitation was performed using colony-forming units per milliliter (CFU / mL) count. Statistical analysis was performed at the significance level of 0.05. Results: No bacterial growth was observed in the negative control. All positive controls demonstrated bacterial growth; S1 from all teeth were positive for bacteria with no significant difference. The post-hoc analysis showed G+1 promoting a significantly higher disinfection than G-1 (p<0,05) and G-1 similar disinfection to G0 (P=962). Conclusion: Instrumentation as close as possible to major foramen or beyond it improves decontamination in oval root canals with reciprocating instrumentation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14765 10.33448/rsd-v10i5.14765 |
url |
https://rsdjournal.org/index.php/rsd/article/view/14765 |
identifier_str_mv |
10.33448/rsd-v10i5.14765 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/14765/13195 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 5; e9810514765 Research, Society and Development; Vol. 10 Núm. 5; e9810514765 Research, Society and Development; v. 10 n. 5; e9810514765 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052675999662080 |