Bioconversion of coffee ground to lipase by filamentous fungi isolated from the Igarassu River in the State of Pernambuco, Brazil

Detalhes bibliográficos
Autor(a) principal: França, Eduardo da Silva
Data de Publicação: 2022
Outros Autores: Souza, Adriana Ferreira, Lemos, Diego Guedes de Lima, Lins, Uiara Maria de Barros Lira, Campos-Takaki, Galba Maria de, Silva , Carlos Alberto Alves da, Lima , Marcos Antônio Barbosa de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/27515
Resumo: Most lipases used for commercial purposes are isolated from microorganisms due to their stability and easy recovery, new residues have been studied to obtain enzymes to reduce production costs. Therefore, this work investigated the biotechnological potential of filamentous fungi isolated from the Igarassu River in the conversion of coffee grounds into lipase in solid state fermentation. The fungi were isolated, identified and submitted to a preliminary assay with 10g of coffee grounds, moisture 60% at 28 °C for 144 h to select the filamentous fungus with the highest potential for lipase production. A factorial design of 23 was carried out to evaluate the influence of the variables moisture, temperature and residue concentration on lipase production by the selected species. Five filamentous fungi were isolated and identified: A. flavus UCP 0316, A. fumigatus UCP 0327, P. variotii UCP 0334, M. hiemalis f. luteis UCP 0343 and A. foetidus UCP 0360. All filamentous fungi cultivated on coffee grounds were able to grow and produce lipase, however A. foetidus exhibited higher enzymatic activity of 514.29 U/mL. We observed that the production of lipase was higher (2941.87 U/mL) when A. foetidus was cultivated in 25 g of coffee grounds, 37 °C and 50% moisture. Therefore, we emphasize that coffee grounds are a promising agro-industrial residue to lipase production, contributing to the reduction of environmental pollution and generating value-added products for the industry.
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spelling Bioconversion of coffee ground to lipase by filamentous fungi isolated from the Igarassu River in the State of Pernambuco, BrazilBioconversión de posos de café a lipasa por hungos filamentosos aislados del Rio Igarassu en el Estado de Pernambuco, BrasilBioconversão da borra de café a lipase por fungos filamentosos isolados do Rio Igarassu no Estado de Pernambuco, BrasilMicrorganismosFermentação em estado sólidoResíduos-agroindustrial.MicroorganismosFermentación en estado sólidoResiduos agroindustriales.MicrorganismsSolid state fermentationAgro-industrial residues.Most lipases used for commercial purposes are isolated from microorganisms due to their stability and easy recovery, new residues have been studied to obtain enzymes to reduce production costs. Therefore, this work investigated the biotechnological potential of filamentous fungi isolated from the Igarassu River in the conversion of coffee grounds into lipase in solid state fermentation. The fungi were isolated, identified and submitted to a preliminary assay with 10g of coffee grounds, moisture 60% at 28 °C for 144 h to select the filamentous fungus with the highest potential for lipase production. A factorial design of 23 was carried out to evaluate the influence of the variables moisture, temperature and residue concentration on lipase production by the selected species. Five filamentous fungi were isolated and identified: A. flavus UCP 0316, A. fumigatus UCP 0327, P. variotii UCP 0334, M. hiemalis f. luteis UCP 0343 and A. foetidus UCP 0360. All filamentous fungi cultivated on coffee grounds were able to grow and produce lipase, however A. foetidus exhibited higher enzymatic activity of 514.29 U/mL. We observed that the production of lipase was higher (2941.87 U/mL) when A. foetidus was cultivated in 25 g of coffee grounds, 37 °C and 50% moisture. Therefore, we emphasize that coffee grounds are a promising agro-industrial residue to lipase production, contributing to the reduction of environmental pollution and generating value-added products for the industry.La mayoría de las lipasas utilizadas con fines comerciales son aisladas de microorganismos debido a su estabilidad y fácil recuperación, se han estudiado nuevos residuos para obtener enzimas que reduzcan los costos de producción. Por lo tanto, este trabajo investigó el potencial biotecnológico de hongos filamentosos aislados del río Igarassu en la conversión de posos de café en lipasa en fermentación en estado sólido. Los hongos fueron aislados, identificados y sometidos a un ensayo preliminar con 10g de café molido, 60% de humedad a 28 °C durante 144 h para seleccionar el hongo filamentoso con mayor potencial de producción de lipasa. Se realizó un diseño factorial de 23 para evaluar la influencia de las variables humedad, temperatura y concentración de residuos en la producción de lipasa por parte de las especies seleccionadas. Se aislaron e identificaron cinco hongos filamentosos: A. flavus UCP 0316, A. fumigatus UCP 0327, P. variotii UCP 0334, M. hiemalis f. luteis UCP 0343 y A. foetidus UCP 0360. Todos los hongos filamentosos cultivados en posos de café pudieron crecer y producir lipasa, sin embargo, A. foetidus exhibió una actividad enzimática más alta de 514.29 U/mL. Observamos que la producción de lipasa fue mayor (2941.87 U/mL) cuando se cultivó A. foetidus en 25 g de café molido, 37 °C y 50% de humedad. Por lo tanto, destacamos que los posos de café son un residuo agroindustrial promisorio para la producción de lipasa, contribuyendo a la reducción de la contaminación ambiental y generando productos con valor agregado para la industria.A maioria das lipases usadas para fins comerciais são isoladas de microrganismos devido a sua estabilidade e fácil recuperação, novos resíduos têm sido estudados para obtenção de enzima para redução dos custos de produção. Portanto, este trabalho investigou o potencial biotecnológico de fungos filamentosos isolados do rio Igarassu na conversão da borra de café em lipase em fermentação em estado sólido. Os fungos foram isolados, identificados e submetidos a um ensaio preliminar com 10g da borra de café, umidade 60% em 28 °C por 144 h para selecionar o fungo filamentoso com maior potencial de produção de lipase. Um planejamento fatorial de 23 foi realizado para avaliar a influência das variáveis umidade, temperatura e concentração do resíduo na produção de lipase pela espécie selecionada. Foram isolados e identificados 5 fungos filamentosos: A. flavus UCP 0316, A. fumigatus UCP 0327, P. variotii UCP 0334, M. hiemalis f. luteis UCP 0343 e A. foetidus UCP 0360. Todos os fungos filamentosos cultivados em borra de café foram capazes de crescer e produzir lipase, porém A. foetidus exibiu maior atividade enzimática de 514,29 U/mL. Observamos que a produção de lipase foi mais alta (2941,87 U/mL) quando A. foetidus foi cultivado em 25 g de borra de café, 37 °C e 50% de umidade. Sendo assim, destacamos que a borra de café é um resíduo agroindustrial promissor para a produção de lipase, contribuindo na redução da poluição ambiental e gerando produtos de valor agregado à indústria.Research, Society and Development2022-03-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/2751510.33448/rsd-v11i4.27515Research, Society and Development; Vol. 11 No. 4; e36011427515Research, Society and Development; Vol. 11 Núm. 4; e36011427515Research, Society and Development; v. 11 n. 4; e360114275152525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/27515/23985Copyright (c) 2022 Eduardo da Silva França; Adriana Ferreira Souza; Diego Guedes de Lima Lemos; Uiara Maria de Barros Lira Lins; Galba Maria de Campos-Takaki; Carlos Alberto Alves da Silva ; Marcos Antônio Barbosa de Lima https://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFrança, Eduardo da SilvaSouza, Adriana Ferreira Lemos, Diego Guedes de Lima Lins, Uiara Maria de Barros LiraCampos-Takaki, Galba Maria de Silva , Carlos Alberto Alves da Lima , Marcos Antônio Barbosa de 2022-03-27T17:17:09Zoai:ojs.pkp.sfu.ca:article/27515Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:45:13.959447Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Bioconversion of coffee ground to lipase by filamentous fungi isolated from the Igarassu River in the State of Pernambuco, Brazil
Bioconversión de posos de café a lipasa por hungos filamentosos aislados del Rio Igarassu en el Estado de Pernambuco, Brasil
Bioconversão da borra de café a lipase por fungos filamentosos isolados do Rio Igarassu no Estado de Pernambuco, Brasil
title Bioconversion of coffee ground to lipase by filamentous fungi isolated from the Igarassu River in the State of Pernambuco, Brazil
spellingShingle Bioconversion of coffee ground to lipase by filamentous fungi isolated from the Igarassu River in the State of Pernambuco, Brazil
França, Eduardo da Silva
Microrganismos
Fermentação em estado sólido
Resíduos-agroindustrial.
Microorganismos
Fermentación en estado sólido
Residuos agroindustriales.
Microrganisms
Solid state fermentation
Agro-industrial residues.
title_short Bioconversion of coffee ground to lipase by filamentous fungi isolated from the Igarassu River in the State of Pernambuco, Brazil
title_full Bioconversion of coffee ground to lipase by filamentous fungi isolated from the Igarassu River in the State of Pernambuco, Brazil
title_fullStr Bioconversion of coffee ground to lipase by filamentous fungi isolated from the Igarassu River in the State of Pernambuco, Brazil
title_full_unstemmed Bioconversion of coffee ground to lipase by filamentous fungi isolated from the Igarassu River in the State of Pernambuco, Brazil
title_sort Bioconversion of coffee ground to lipase by filamentous fungi isolated from the Igarassu River in the State of Pernambuco, Brazil
author França, Eduardo da Silva
author_facet França, Eduardo da Silva
Souza, Adriana Ferreira
Lemos, Diego Guedes de Lima
Lins, Uiara Maria de Barros Lira
Campos-Takaki, Galba Maria de
Silva , Carlos Alberto Alves da
Lima , Marcos Antônio Barbosa de
author_role author
author2 Souza, Adriana Ferreira
Lemos, Diego Guedes de Lima
Lins, Uiara Maria de Barros Lira
Campos-Takaki, Galba Maria de
Silva , Carlos Alberto Alves da
Lima , Marcos Antônio Barbosa de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv França, Eduardo da Silva
Souza, Adriana Ferreira
Lemos, Diego Guedes de Lima
Lins, Uiara Maria de Barros Lira
Campos-Takaki, Galba Maria de
Silva , Carlos Alberto Alves da
Lima , Marcos Antônio Barbosa de
dc.subject.por.fl_str_mv Microrganismos
Fermentação em estado sólido
Resíduos-agroindustrial.
Microorganismos
Fermentación en estado sólido
Residuos agroindustriales.
Microrganisms
Solid state fermentation
Agro-industrial residues.
topic Microrganismos
Fermentação em estado sólido
Resíduos-agroindustrial.
Microorganismos
Fermentación en estado sólido
Residuos agroindustriales.
Microrganisms
Solid state fermentation
Agro-industrial residues.
description Most lipases used for commercial purposes are isolated from microorganisms due to their stability and easy recovery, new residues have been studied to obtain enzymes to reduce production costs. Therefore, this work investigated the biotechnological potential of filamentous fungi isolated from the Igarassu River in the conversion of coffee grounds into lipase in solid state fermentation. The fungi were isolated, identified and submitted to a preliminary assay with 10g of coffee grounds, moisture 60% at 28 °C for 144 h to select the filamentous fungus with the highest potential for lipase production. A factorial design of 23 was carried out to evaluate the influence of the variables moisture, temperature and residue concentration on lipase production by the selected species. Five filamentous fungi were isolated and identified: A. flavus UCP 0316, A. fumigatus UCP 0327, P. variotii UCP 0334, M. hiemalis f. luteis UCP 0343 and A. foetidus UCP 0360. All filamentous fungi cultivated on coffee grounds were able to grow and produce lipase, however A. foetidus exhibited higher enzymatic activity of 514.29 U/mL. We observed that the production of lipase was higher (2941.87 U/mL) when A. foetidus was cultivated in 25 g of coffee grounds, 37 °C and 50% moisture. Therefore, we emphasize that coffee grounds are a promising agro-industrial residue to lipase production, contributing to the reduction of environmental pollution and generating value-added products for the industry.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/27515
10.33448/rsd-v11i4.27515
url https://rsdjournal.org/index.php/rsd/article/view/27515
identifier_str_mv 10.33448/rsd-v11i4.27515
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/27515/23985
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 11 No. 4; e36011427515
Research, Society and Development; Vol. 11 Núm. 4; e36011427515
Research, Society and Development; v. 11 n. 4; e36011427515
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
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