Effects of hydroxyapatite associated to collagen type I graft in bone repair of critical defects in rabbits
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/30243 |
Resumo: | The aim of this investigation was to evaluate the influences of a malleable bone graft composed by hydroxyapatite and collagen type I in bone formation. Three surgical sites of critical size were prepared in twelve rabbits’ calvarias. The control defect was fulfilled with blood clot and the experimental defects were fulfilled with the graft. The euthanasia occurred in three times: 4, 8 and 12 weeks after the surgical procedures. The specimens were analyzed in light, fluorescence and scanning electronic microscopes. The quantitative evaluation was made by histomorphometric analysis, in order to calculate the percentage of new bone formed and the area of the hydroxyapatite particles. The results were submitted to two way ANOVA test and Tukey`s post hoc. New bone formation was accelerated in early stages of the healing process. It was seen more intensive bone formation in the periosteum side than in the dura mater membrane side in all groups. Besides that, in experimental sides the new bone was surrounding the hydroxyapatite particles with minimal amount of inflammatory cells, which confirmed the biocompatibility and osteoconduction properties of this material. Besides that, the hydroxyapatite particles showed gradual and progressive absorption at the same time that the bone was being formed. |
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Effects of hydroxyapatite associated to collagen type I graft in bone repair of critical defects in rabbitsEfectos de la hidroxiapatita asociada al injerto de colágeno tipo I en la reparación ósea de defectos críticos en conejosEfeitos da hidroxiapatita associada ao enxerto de colágeno tipo I no reparo ósseo de defeitos críticos em coelhosHidroxiapatitaColágenoOsso.HidroxiapatitaColágenoHueso.HydroxyapatiteCollagenBoner.The aim of this investigation was to evaluate the influences of a malleable bone graft composed by hydroxyapatite and collagen type I in bone formation. Three surgical sites of critical size were prepared in twelve rabbits’ calvarias. The control defect was fulfilled with blood clot and the experimental defects were fulfilled with the graft. The euthanasia occurred in three times: 4, 8 and 12 weeks after the surgical procedures. The specimens were analyzed in light, fluorescence and scanning electronic microscopes. The quantitative evaluation was made by histomorphometric analysis, in order to calculate the percentage of new bone formed and the area of the hydroxyapatite particles. The results were submitted to two way ANOVA test and Tukey`s post hoc. New bone formation was accelerated in early stages of the healing process. It was seen more intensive bone formation in the periosteum side than in the dura mater membrane side in all groups. Besides that, in experimental sides the new bone was surrounding the hydroxyapatite particles with minimal amount of inflammatory cells, which confirmed the biocompatibility and osteoconduction properties of this material. Besides that, the hydroxyapatite particles showed gradual and progressive absorption at the same time that the bone was being formed.El objetivo de esta investigación fue evaluar las influencias de un injerto óseo maleable compuesto por hidroxiapatita y colágeno tipo I en la formación ósea. Se prepararon tres sitios quirúrgicos de tamaño crítico en doce calvarias de conejos. El defecto de control se cumplió con el coágulo de sangre y los defectos experimentales se cumplieron con el injerto. La eutanasia ocurrió en tres tiempos: 4, 8 y 12 semanas después de los procedimientos quirúrgicos. Los especímenes fueron analizados en microscopios electrónicos de luz, fluorescencia y barrido. La evaluación cuantitativa se hizo por análisis histomorfométrico, para calcular el porcentaje de hueso nuevo formado y el área de las partículas de hidroxiapatita. Los resultados fueron sometidos a la prueba ANOVA de dos vías y post hoc de Tukey. La formación de hueso nuevo se aceleró en las primeras etapas del proceso de curación. Se observó una formación ósea más intensa en el lado del periostio que en el lado de la membrana de la duramadre en todos los grupos. Además, en los lados experimentales, el hueso nuevo rodeaba las partículas de hidroxiapatita con una cantidad mínima de células inflamatorias, lo que confirmó las propiedades de biocompatibilidad y osteoconducción de este material. Además de eso, las partículas de hidroxiapatita mostraron una absorción gradual y progresiva al mismo tiempo que se iba formando el hueso.O objetivo desta investigação foi avaliar as influências de um enxerto ósseo maleável composto por hidroxiapatita e colágeno tipo I na formação óssea. Três sítios cirúrgicos de tamanho crítico foram preparados em doze calvárias de coelhos. O defeito controle foi preenchido com coágulo sanguíneo e os defeitos experimentais foram preenchidos com o enxerto. A eutanásia ocorreu em três momentos: 4, 8 e 12 semanas após os procedimentos cirúrgicos. Os espécimes foram analisados em microscópios eletrônicos de luz, fluorescência e varredura. A avaliação quantitativa foi feita por análise histomorfométrica, a fim de calcular a porcentagem de osso novo formado e a área das partículas de hidroxiapatita. Os resultados foram submetidos ao teste ANOVA de duas vias e post hoc de Tukey. A nova formação óssea foi acelerada nos estágios iniciais do processo de cicatrização. Foi observada formação óssea mais intensa no lado do periósteo do que no lado da membrana da dura-máter em todos os grupos. Além disso, nas vertentes experimentais o novo osso circundava as partículas de hidroxiapatita com quantidade mínima de células inflamatórias, o que confirmava a biocompatibilidade e as propriedades de osteocondução deste material. Além disso, as partículas de hidroxiapatita apresentaram absorção gradual e progressiva ao mesmo tempo em que o osso estava sendo formado.Research, Society and Development2022-05-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3024310.33448/rsd-v11i7.30243Research, Society and Development; Vol. 11 No. 7; e45411730243Research, Society and Development; Vol. 11 Núm. 7; e45411730243Research, Society and Development; v. 11 n. 7; e454117302432525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIenghttps://rsdjournal.org/index.php/rsd/article/view/30243/26048Copyright (c) 2022 Fernanda Mara de Paiva Bertoli ; Lílian de Mello Gil ; Leonardo Rodrigues de Andrade ; Matheus Melo Pithon; Jayme Bordini Júnior ; Matilde da Cunha Gonçalves Nojimahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBertoli , Fernanda Mara de Paiva Gil , Lílian de Mello Andrade , Leonardo Rodrigues de Pithon, Matheus MeloBordini Júnior , JaymeNojima, Matilde da Cunha Gonçalves 2022-06-06T15:12:05Zoai:ojs.pkp.sfu.ca:article/30243Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:47:03.764901Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Effects of hydroxyapatite associated to collagen type I graft in bone repair of critical defects in rabbits Efectos de la hidroxiapatita asociada al injerto de colágeno tipo I en la reparación ósea de defectos críticos en conejos Efeitos da hidroxiapatita associada ao enxerto de colágeno tipo I no reparo ósseo de defeitos críticos em coelhos |
| title |
Effects of hydroxyapatite associated to collagen type I graft in bone repair of critical defects in rabbits |
| spellingShingle |
Effects of hydroxyapatite associated to collagen type I graft in bone repair of critical defects in rabbits Bertoli , Fernanda Mara de Paiva Hidroxiapatita Colágeno Osso. Hidroxiapatita Colágeno Hueso. Hydroxyapatite Collagen Boner. |
| title_short |
Effects of hydroxyapatite associated to collagen type I graft in bone repair of critical defects in rabbits |
| title_full |
Effects of hydroxyapatite associated to collagen type I graft in bone repair of critical defects in rabbits |
| title_fullStr |
Effects of hydroxyapatite associated to collagen type I graft in bone repair of critical defects in rabbits |
| title_full_unstemmed |
Effects of hydroxyapatite associated to collagen type I graft in bone repair of critical defects in rabbits |
| title_sort |
Effects of hydroxyapatite associated to collagen type I graft in bone repair of critical defects in rabbits |
| author |
Bertoli , Fernanda Mara de Paiva |
| author_facet |
Bertoli , Fernanda Mara de Paiva Gil , Lílian de Mello Andrade , Leonardo Rodrigues de Pithon, Matheus Melo Bordini Júnior , Jayme Nojima, Matilde da Cunha Gonçalves |
| author_role |
author |
| author2 |
Gil , Lílian de Mello Andrade , Leonardo Rodrigues de Pithon, Matheus Melo Bordini Júnior , Jayme Nojima, Matilde da Cunha Gonçalves |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Bertoli , Fernanda Mara de Paiva Gil , Lílian de Mello Andrade , Leonardo Rodrigues de Pithon, Matheus Melo Bordini Júnior , Jayme Nojima, Matilde da Cunha Gonçalves |
| dc.subject.por.fl_str_mv |
Hidroxiapatita Colágeno Osso. Hidroxiapatita Colágeno Hueso. Hydroxyapatite Collagen Boner. |
| topic |
Hidroxiapatita Colágeno Osso. Hidroxiapatita Colágeno Hueso. Hydroxyapatite Collagen Boner. |
| description |
The aim of this investigation was to evaluate the influences of a malleable bone graft composed by hydroxyapatite and collagen type I in bone formation. Three surgical sites of critical size were prepared in twelve rabbits’ calvarias. The control defect was fulfilled with blood clot and the experimental defects were fulfilled with the graft. The euthanasia occurred in three times: 4, 8 and 12 weeks after the surgical procedures. The specimens were analyzed in light, fluorescence and scanning electronic microscopes. The quantitative evaluation was made by histomorphometric analysis, in order to calculate the percentage of new bone formed and the area of the hydroxyapatite particles. The results were submitted to two way ANOVA test and Tukey`s post hoc. New bone formation was accelerated in early stages of the healing process. It was seen more intensive bone formation in the periosteum side than in the dura mater membrane side in all groups. Besides that, in experimental sides the new bone was surrounding the hydroxyapatite particles with minimal amount of inflammatory cells, which confirmed the biocompatibility and osteoconduction properties of this material. Besides that, the hydroxyapatite particles showed gradual and progressive absorption at the same time that the bone was being formed. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-05-31 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/30243 10.33448/rsd-v11i7.30243 |
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https://rsdjournal.org/index.php/rsd/article/view/30243 |
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10.33448/rsd-v11i7.30243 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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https://rsdjournal.org/index.php/rsd/article/view/30243/26048 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 11 No. 7; e45411730243 Research, Society and Development; Vol. 11 Núm. 7; e45411730243 Research, Society and Development; v. 11 n. 7; e45411730243 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052795590803456 |