Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models
| Autor(a) principal: | |
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| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Research, Society and Development |
| Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/16060 |
Resumo: | Lippia species (Verbenaceae) are widely used on folk medicine to treat respiratory and gastrointestinal disorders. In this work, we evaluated the relaxant effect of the essential oil from Lippia microphylla Cham. (LM‑EO) and its major components on guinea pig ileum as well as elucidate the involved mechanisms. LM-EO inhibited phasic contractions as well as relaxed tonic contractions induced by several contractile agents on guinea pig ileum. Similar results were observed in tests using thymol and carvacrol, the major constituents of LM-EO. Voltage-dependent calcium channel (CaV) blockade was suggested since LM-EO shifted to the right non parallelly CaCl2-induced cumulative contractions and confirmed when LM-EO relaxed the ileum pre contracted by S-(-)-Bay K8644, a selective CaV agonist. On circular layer, LM-EO inhibited phasic contractions, being equipotent to the effect observed in the intact ileum. In the myocytes from ileum longitudinal layer, LM-EO reduced fluorescence intensity induced by histamine, similarly to verapamil (CaV blocker), indicating a decrease in cytosolic concentration of Ca2+ ([Ca2+]c). In conclusion, LM-EO presents a spasmolytic activity on guinea pig ileum by CaV blockade and [Ca2+]c reduction. These results present a rationale for the use of L. microphylla as a potential product to intestinal disorders. |
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Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular modelsLa actividad espasmolítica del aceite esencial obtenido de Lippia microphylla Cham. (Verbenaceae) está mediada por la modulación de la señalización del calcio en modelos animales y celularesA atividade espasmolítica do óleo essencial obtido de Lippia microphylla Cham. (Verbenaceae) é mediada pela modulação da sinalização do cálcio em modelos animais e celularesLippia microphylla ChamVerbenaceaeÓleo essencialTimolCarvacrolMúsculo liso intestinal.Lippia microphylla ChamVerbenaceaeEssential oilTimolCarvacrolMúsculo liso intestinal.Lippia microphylla ChamVerbenaceaeAceite essencialTimol; CarvacrolMúsculo liso intestinal.Lippia species (Verbenaceae) are widely used on folk medicine to treat respiratory and gastrointestinal disorders. In this work, we evaluated the relaxant effect of the essential oil from Lippia microphylla Cham. (LM‑EO) and its major components on guinea pig ileum as well as elucidate the involved mechanisms. LM-EO inhibited phasic contractions as well as relaxed tonic contractions induced by several contractile agents on guinea pig ileum. Similar results were observed in tests using thymol and carvacrol, the major constituents of LM-EO. Voltage-dependent calcium channel (CaV) blockade was suggested since LM-EO shifted to the right non parallelly CaCl2-induced cumulative contractions and confirmed when LM-EO relaxed the ileum pre contracted by S-(-)-Bay K8644, a selective CaV agonist. On circular layer, LM-EO inhibited phasic contractions, being equipotent to the effect observed in the intact ileum. In the myocytes from ileum longitudinal layer, LM-EO reduced fluorescence intensity induced by histamine, similarly to verapamil (CaV blocker), indicating a decrease in cytosolic concentration of Ca2+ ([Ca2+]c). In conclusion, LM-EO presents a spasmolytic activity on guinea pig ileum by CaV blockade and [Ca2+]c reduction. These results present a rationale for the use of L. microphylla as a potential product to intestinal disorders.Las especies del género Lippia (Verbenaceae) se utilizan ampliamente en la medicina tradicional para tratar desórdenes respiratorios y gastrointestinales. En este estudio, el efecto relajante del aceite esencial obtenido de Lippia microphylla Cham. (LM-EO) y sus componentes principales en el íleon de cobaya, así como los mecanismos implicados. LM-EO inhibió las contracciones fásicas y relajó la tonicidad inducida por varios agentes contráctiles en el íleon de cobaya. Se observaron resultados similares en los protocolos que utilizan timol y carvacrol, los componentes principales de LM-EO. Se sugirió el bloqueo de los canales de calcio dependientes de voltaje (CaV) ya que LM-EO se desplazó hacia la derecha y de manera no paralela las contracciones acumulativas inducidas por CaCl2 y se confirmó cuando LM-EO relajó el íleon precontraído por S‑(‑)‑Bay K8644, un agonista selectivo de CaV. En la capa muscular circular del íleon, LM-EO inhibió las contracciones fásicas, siendo equipotente en relación a los efectos sobre el íleon intacto. En miocitos aislados de la capa de íleon longitudinal, LM-EO redujo la intensidad de la fluorescencia inducida por histamina, de manera similar al verapamilo (bloqueador de CaV), lo que indica una reducción en la concentración de calcio citosólico ([Ca2+]c). Por tanto, LM-EO presenta actividad espasmolítica en el íleo de cobaya al bloquear el CaV y la consiguiente reducción de [Ca2+]c. Estos resultados se muestran como una justificación para el uso de L. microphylla como producto terapéutico en trastornos intestinales.As espécies do gênero Lippia (Verbenaceae) são amplamente usadas na medicina tradicional no tratamento de desordens respiratórias e gastrointestinais. Neste estudo, avaliou-se o efeito relaxante do óleo essencial obtido de Lippia microphylla Cham. (LM‑EO) e seus componentes majoritários em íleo isolado de cobaias bem como elucidou‑se os mecanismos envolvidos. LM-EO inibiu as contrações fásicas e relaxou as tônicas induzidas por vários agentes contráteis em íleo de cobaia. Resultados semelhantes foram observados nos protocolos utilizando-se timol e carvacrol, os componentes majoritários de LM-EO. O bloqueio dos canais de cálcio dependentes de voltagem (CaV) foi sugerido uma vez que LM-EO deslocou para a direita e de maneira não paralela as contrações cumulativas induzidas por CaCl2 e confirmou-se quando LM-EO relaxou o íleo pré-contraído por S‑(‑)‑Bay K8644, um agonista seletivo dos CaV. Na camada muscular circular do íleo, LM-EO inibiu as contrações fásicas, sendo equipotente em relação aos efeitos no íleo íntegro. No miócitos isolados da camada longitudinal do íleo, LM-EO reduziu a intensidade da fluorescência induzida por histamina, semelhantemente ao verapamil (bloqueador CaV), indicando uma redução na concentração citosólica de cálcio ([Ca2+]c). Dessa forma, LM-EO apresenta uma atividade espasmolítica em íleo de cobaia pelo bloqueio dos CaV e consequente redução da [Ca2+]c. Esses resultados demostram-se como uma justificativa para o uso de L. microphylla como um produto terapêutico nas desordens intestinais.Research, Society and Development2021-06-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1606010.33448/rsd-v10i7.16060Research, Society and Development; Vol. 10 No. 7; e0410716060Research, Society and Development; Vol. 10 Núm. 7; e0410716060Research, Society and Development; v. 10 n. 7; e04107160602525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/16060/14466Copyright (c) 2021 Gislaine Alves de Oliveira; Joedna Cavalcante Pereira; Italo Rossi Roseno Martins; Ana Carolina de Carvalho Correia; Rafael de Almeida Travassos; Maria da Conceição Correia Silva; Iara Leão Luna de Souza; Josean Fechine Tavares; Edgar Julian Paredes-Gamero; Bagnólia Araújo da Silvahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira, Gislaine Alves dePereira, Joedna CavalcanteMartins, Italo Rossi RosenoCorreia, Ana Carolina de CarvalhoTravassos, Rafael de AlmeidaSilva, Maria da Conceição CorreiaSouza, Iara Leão Luna deTavares, Josean FechineParedes-Gamero, Edgar Julian Silva, Bagnólia Araújo da2021-07-18T21:07:03Zoai:ojs.pkp.sfu.ca:article/16060Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:36:43.540255Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
| dc.title.none.fl_str_mv |
Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models La actividad espasmolítica del aceite esencial obtenido de Lippia microphylla Cham. (Verbenaceae) está mediada por la modulación de la señalización del calcio en modelos animales y celulares A atividade espasmolítica do óleo essencial obtido de Lippia microphylla Cham. (Verbenaceae) é mediada pela modulação da sinalização do cálcio em modelos animais e celulares |
| title |
Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models |
| spellingShingle |
Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models Oliveira, Gislaine Alves de Lippia microphylla Cham Verbenaceae Óleo essencial Timol Carvacrol Músculo liso intestinal. Lippia microphylla Cham Verbenaceae Essential oil Timol Carvacrol Músculo liso intestinal. Lippia microphylla Cham Verbenaceae Aceite essencial Timol ; Carvacrol Músculo liso intestinal. |
| title_short |
Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models |
| title_full |
Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models |
| title_fullStr |
Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models |
| title_full_unstemmed |
Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models |
| title_sort |
Spasmolytic activity of essential oil from Lippia microphylla Cham. (Verbenaceae) is mediated by modulation of Ca2+ signaling on animal and cellular models |
| author |
Oliveira, Gislaine Alves de |
| author_facet |
Oliveira, Gislaine Alves de Pereira, Joedna Cavalcante Martins, Italo Rossi Roseno Correia, Ana Carolina de Carvalho Travassos, Rafael de Almeida Silva, Maria da Conceição Correia Souza, Iara Leão Luna de Tavares, Josean Fechine Paredes-Gamero, Edgar Julian Silva, Bagnólia Araújo da |
| author_role |
author |
| author2 |
Pereira, Joedna Cavalcante Martins, Italo Rossi Roseno Correia, Ana Carolina de Carvalho Travassos, Rafael de Almeida Silva, Maria da Conceição Correia Souza, Iara Leão Luna de Tavares, Josean Fechine Paredes-Gamero, Edgar Julian Silva, Bagnólia Araújo da |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Gislaine Alves de Pereira, Joedna Cavalcante Martins, Italo Rossi Roseno Correia, Ana Carolina de Carvalho Travassos, Rafael de Almeida Silva, Maria da Conceição Correia Souza, Iara Leão Luna de Tavares, Josean Fechine Paredes-Gamero, Edgar Julian Silva, Bagnólia Araújo da |
| dc.subject.por.fl_str_mv |
Lippia microphylla Cham Verbenaceae Óleo essencial Timol Carvacrol Músculo liso intestinal. Lippia microphylla Cham Verbenaceae Essential oil Timol Carvacrol Músculo liso intestinal. Lippia microphylla Cham Verbenaceae Aceite essencial Timol ; Carvacrol Músculo liso intestinal. |
| topic |
Lippia microphylla Cham Verbenaceae Óleo essencial Timol Carvacrol Músculo liso intestinal. Lippia microphylla Cham Verbenaceae Essential oil Timol Carvacrol Músculo liso intestinal. Lippia microphylla Cham Verbenaceae Aceite essencial Timol ; Carvacrol Músculo liso intestinal. |
| description |
Lippia species (Verbenaceae) are widely used on folk medicine to treat respiratory and gastrointestinal disorders. In this work, we evaluated the relaxant effect of the essential oil from Lippia microphylla Cham. (LM‑EO) and its major components on guinea pig ileum as well as elucidate the involved mechanisms. LM-EO inhibited phasic contractions as well as relaxed tonic contractions induced by several contractile agents on guinea pig ileum. Similar results were observed in tests using thymol and carvacrol, the major constituents of LM-EO. Voltage-dependent calcium channel (CaV) blockade was suggested since LM-EO shifted to the right non parallelly CaCl2-induced cumulative contractions and confirmed when LM-EO relaxed the ileum pre contracted by S-(-)-Bay K8644, a selective CaV agonist. On circular layer, LM-EO inhibited phasic contractions, being equipotent to the effect observed in the intact ileum. In the myocytes from ileum longitudinal layer, LM-EO reduced fluorescence intensity induced by histamine, similarly to verapamil (CaV blocker), indicating a decrease in cytosolic concentration of Ca2+ ([Ca2+]c). In conclusion, LM-EO presents a spasmolytic activity on guinea pig ileum by CaV blockade and [Ca2+]c reduction. These results present a rationale for the use of L. microphylla as a potential product to intestinal disorders. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-06-11 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://rsdjournal.org/index.php/rsd/article/view/16060 10.33448/rsd-v10i7.16060 |
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https://rsdjournal.org/index.php/rsd/article/view/16060 |
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10.33448/rsd-v10i7.16060 |
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por |
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por |
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https://rsdjournal.org/index.php/rsd/article/view/16060/14466 |
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https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development; Vol. 10 No. 7; e0410716060 Research, Society and Development; Vol. 10 Núm. 7; e0410716060 Research, Society and Development; v. 10 n. 7; e0410716060 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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