Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradation
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/31559 |
Resumo: | Activities involving the use of petroleum and its derivatives have been a cause of constant concern for the damage caused to ecosystems, due to the high toxicity and difficult degradation of these compounds. Consequently, several decontamination methods have been carried out, highlighting among them bioremediation, an ecologically viable technique that aims at the application of microorganisms and/or their products to remove or degrade petroleum derivatives and minimize the harmful effects in the impacted areas. In this sense, the use of microorganisms isolated from the ecosystem itself constitutes a promising strategy, considering that they already have mechanisms of resistance to these adverse environmental conditions. Thus, the aim of this work was to evaluate the biodegradation potential of petroderivatives of filamentous fungi isolated from mangrove sediments in the State of Pernambuco, contaminated with the spill that occurred in August 2019 on the coast of Northeast Brazil. For this, four fungal isolates belonging to the genera Aspergillus sp., Penicillium sp., Talaromyces sp. and Trichoderma sp. were subjected to acclimatization in Sabouraud Agar medium containing 1-20% of burnt engine oil, and growth was verified at all concentrations after 72 h. Then, the microorganisms acclimated to 20% were selected for the diesel and kerosene biodegradation test, in Bushnell Haas medium containing the redox indicator 2,6-dichlorophenol-indophenol (DCPIP), obtaining results above 75% after 72 h. However, Aspergillus sp. demonstrated the greatest potential, since it reached 88.4% of biodegradation of both petroderivatives. Thus, it is suggested the use of this promising microorganism in bioremediation processes of ecosystems impacted by oil and derivatives. |
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Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradationBioprospección de hongos filamentosos aislados de sedimentos de manglar del estado de Pernambuco para la biodegradación de petroderivadosBioprospecção de fungos filamentosos isolados de sedimentos de mangue do estado de Pernambuco para biodegradação de petroderivadosFilamentous fungiOil spill bioremediationSelection of biodegrading microorganismsEnvironmental recovery.Hongos filamentososBiorremediación de derrames de petróleoSelección de microorganismos biodegradadoresRecuperación ambiental.Fungos filamentososBiorremediação de derramamento de óleoSeleção de micro-organismos biodegradadoresRecuperação ambiental.Activities involving the use of petroleum and its derivatives have been a cause of constant concern for the damage caused to ecosystems, due to the high toxicity and difficult degradation of these compounds. Consequently, several decontamination methods have been carried out, highlighting among them bioremediation, an ecologically viable technique that aims at the application of microorganisms and/or their products to remove or degrade petroleum derivatives and minimize the harmful effects in the impacted areas. In this sense, the use of microorganisms isolated from the ecosystem itself constitutes a promising strategy, considering that they already have mechanisms of resistance to these adverse environmental conditions. Thus, the aim of this work was to evaluate the biodegradation potential of petroderivatives of filamentous fungi isolated from mangrove sediments in the State of Pernambuco, contaminated with the spill that occurred in August 2019 on the coast of Northeast Brazil. For this, four fungal isolates belonging to the genera Aspergillus sp., Penicillium sp., Talaromyces sp. and Trichoderma sp. were subjected to acclimatization in Sabouraud Agar medium containing 1-20% of burnt engine oil, and growth was verified at all concentrations after 72 h. Then, the microorganisms acclimated to 20% were selected for the diesel and kerosene biodegradation test, in Bushnell Haas medium containing the redox indicator 2,6-dichlorophenol-indophenol (DCPIP), obtaining results above 75% after 72 h. However, Aspergillus sp. demonstrated the greatest potential, since it reached 88.4% of biodegradation of both petroderivatives. Thus, it is suggested the use of this promising microorganism in bioremediation processes of ecosystems impacted by oil and derivatives.Las actividades que involucran el uso del petróleo y sus derivados han sido un motivo de preocupación constante por los daños causados a los ecosistemas, debido a la alta toxicidad y difícil degradación de estos compuestos. En consecuencia, se han desarrollado varios métodos de descontaminación, destacándose entre ellos la biorremediación, una técnica ecológicamente viable que tiene como objetivo aplicar microorganismos y/o sus productos para remover o degradar petroderivados y minimizar los efectos nocivos en las áreas impactadas. En este sentido, el uso de microorganismos aislados del propio ecosistema constituye una estrategia promisoria, considerando que ya cuentan con mecanismos de resistencia a esas condiciones ambientales adversas. Así, el objetivo de este trabajo fue evaluar el potencial de biodegradación de petroderivados de hongos filamentosos aislados de sedimentos de manglares en el Estado de Pernambuco, contaminado con el derrame ocurrido en agosto de 2019 en la costa del noreste de Brasil. Para ello, cuatro aislamientos fúngicos pertenecientes a los géneros Aspergillus sp., Penicillium sp., Talaromyces sp. y Trichoderma sp. fueron aclimatados en medio Agar Sabouraud que contenía 1-20% de aceite de motor usado, y se comprobó el crecimiento en todas las concentraciones después de 72 h. Luego, los microorganismos aclimatados al 20% fueron seleccionados para el ensayo de biodegradación de diesel y queroseno, en medio Bushnell Haas conteniendo el indicador redox 2,6-diclorofenol-indofenol (DCPIP), verificándose resultados superiores al 75% después de 72 h. Sin embargo, Aspergillus sp. demostró el mayor potencial, ya que alcanzó un 88,4% de biodegradación de ambos petroderivados. Así, se sugiere el uso de este prometedor microorganismo en procesos de biorremediación de ecosistemas impactados por petróleo y derivados.As atividades que envolvem a utilização do petróleo e seus derivados têm sido motivo de constante preocupação pelos danos causados aos ecossistemas, devido à elevada toxicidade e difícil degradação destes compostos. Consequentemente, diversos métodos de descontaminação vêm sendo desenvolvidos, destacando-se dentre eles a biorremediação, uma técnica ecologicamente viável que visa à aplicação de micro-organismos e/ou seus produtos para remover ou degradar derivados do petróleo e minimizar os efeitos nocivos nas áreas impactadas. Neste sentido, a utilização de micro-organismos isolados do próprio ecossistema constitui uma estratégia promissória, considerando que já possuem mecanismos de resistência a essas condições ambientais adversas. Assim, o objetivo deste trabalho foi avaliar o potencial de biodegradação de petroderivados de fungos filamentosos isolados de sedimentos de mangue do Estado de Pernambuco, contaminados com o derramamento ocorrido em agosto de 2019 na costa do Nordeste brasileiro. Para isso, quatro isolados fúngicos pertencentes aos gêneros Aspergillus sp., Penicillium sp., Talaromyces sp. e Trichoderma sp. foram submetidos a aclimatação em meio Ágar Sabouraud contendo 1-20% de óleo de motor queimado, constatando-se o crescimento em todas as concentrações após 72 h. Em seguida, os micro-organismos aclimatados a 20% foram selecionados para o ensaio de biodegradação de diesel e querosene, no meio Bushnell Haas contendo o indicador redox 2,6-diclorofenol-indofenol (DCPIP), verificando-se resultados acima de 75% após 72 h. Contudo, Aspergillus sp. demonstrou o maior potencial, uma vez que alcançou 88,4% de biodegradação de ambos petroderivados. Assim, sugere-se a utilização deste micro-organismo promissor em processos de biorremediação de ecossistemas impactados por petróleo e derivados.Research, Society and Development2022-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/3155910.33448/rsd-v11i9.31559Research, Society and Development; Vol. 11 No. 9; e11311931559Research, Society and Development; Vol. 11 Núm. 9; e11311931559Research, Society and Development; v. 11 n. 9; e113119315592525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/31559/26973Copyright (c) 2022 Paulo Gustavo Otaviano da Silva; Rafael de Souza Mendonça; Everton Ricardo Carneiro Costa; Galba Maria de Campos-Takaki; Rosileide Fontenele da Silva Andrade; Dayana Montero Rodríguezhttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSilva, Paulo Gustavo Otaviano da Mendonça, Rafael de Souza Costa, Everton Ricardo Carneiro Campos-Takaki, Galba Maria de Andrade, Rosileide Fontenele da Silva Montero Rodríguez, Dayana 2022-07-21T12:36:16Zoai:ojs.pkp.sfu.ca:article/31559Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:47:51.494708Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradation Bioprospección de hongos filamentosos aislados de sedimentos de manglar del estado de Pernambuco para la biodegradación de petroderivados Bioprospecção de fungos filamentosos isolados de sedimentos de mangue do estado de Pernambuco para biodegradação de petroderivados |
title |
Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradation |
spellingShingle |
Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradation Silva, Paulo Gustavo Otaviano da Filamentous fungi Oil spill bioremediation Selection of biodegrading microorganisms Environmental recovery. Hongos filamentosos Biorremediación de derrames de petróleo Selección de microorganismos biodegradadores Recuperación ambiental. Fungos filamentosos Biorremediação de derramamento de óleo Seleção de micro-organismos biodegradadores Recuperação ambiental. |
title_short |
Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradation |
title_full |
Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradation |
title_fullStr |
Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradation |
title_full_unstemmed |
Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradation |
title_sort |
Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradation |
author |
Silva, Paulo Gustavo Otaviano da |
author_facet |
Silva, Paulo Gustavo Otaviano da Mendonça, Rafael de Souza Costa, Everton Ricardo Carneiro Campos-Takaki, Galba Maria de Andrade, Rosileide Fontenele da Silva Montero Rodríguez, Dayana |
author_role |
author |
author2 |
Mendonça, Rafael de Souza Costa, Everton Ricardo Carneiro Campos-Takaki, Galba Maria de Andrade, Rosileide Fontenele da Silva Montero Rodríguez, Dayana |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Paulo Gustavo Otaviano da Mendonça, Rafael de Souza Costa, Everton Ricardo Carneiro Campos-Takaki, Galba Maria de Andrade, Rosileide Fontenele da Silva Montero Rodríguez, Dayana |
dc.subject.por.fl_str_mv |
Filamentous fungi Oil spill bioremediation Selection of biodegrading microorganisms Environmental recovery. Hongos filamentosos Biorremediación de derrames de petróleo Selección de microorganismos biodegradadores Recuperación ambiental. Fungos filamentosos Biorremediação de derramamento de óleo Seleção de micro-organismos biodegradadores Recuperação ambiental. |
topic |
Filamentous fungi Oil spill bioremediation Selection of biodegrading microorganisms Environmental recovery. Hongos filamentosos Biorremediación de derrames de petróleo Selección de microorganismos biodegradadores Recuperación ambiental. Fungos filamentosos Biorremediação de derramamento de óleo Seleção de micro-organismos biodegradadores Recuperação ambiental. |
description |
Activities involving the use of petroleum and its derivatives have been a cause of constant concern for the damage caused to ecosystems, due to the high toxicity and difficult degradation of these compounds. Consequently, several decontamination methods have been carried out, highlighting among them bioremediation, an ecologically viable technique that aims at the application of microorganisms and/or their products to remove or degrade petroleum derivatives and minimize the harmful effects in the impacted areas. In this sense, the use of microorganisms isolated from the ecosystem itself constitutes a promising strategy, considering that they already have mechanisms of resistance to these adverse environmental conditions. Thus, the aim of this work was to evaluate the biodegradation potential of petroderivatives of filamentous fungi isolated from mangrove sediments in the State of Pernambuco, contaminated with the spill that occurred in August 2019 on the coast of Northeast Brazil. For this, four fungal isolates belonging to the genera Aspergillus sp., Penicillium sp., Talaromyces sp. and Trichoderma sp. were subjected to acclimatization in Sabouraud Agar medium containing 1-20% of burnt engine oil, and growth was verified at all concentrations after 72 h. Then, the microorganisms acclimated to 20% were selected for the diesel and kerosene biodegradation test, in Bushnell Haas medium containing the redox indicator 2,6-dichlorophenol-indophenol (DCPIP), obtaining results above 75% after 72 h. However, Aspergillus sp. demonstrated the greatest potential, since it reached 88.4% of biodegradation of both petroderivatives. Thus, it is suggested the use of this promising microorganism in bioremediation processes of ecosystems impacted by oil and derivatives. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/31559 10.33448/rsd-v11i9.31559 |
url |
https://rsdjournal.org/index.php/rsd/article/view/31559 |
identifier_str_mv |
10.33448/rsd-v11i9.31559 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/31559/26973 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 11 No. 9; e11311931559 Research, Society and Development; Vol. 11 Núm. 9; e11311931559 Research, Society and Development; v. 11 n. 9; e11311931559 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
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Universidade Federal de Itajubá (UNIFEI) |
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UNIFEI |
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UNIFEI |
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Research, Society and Development |
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Research, Society and Development |
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Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
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rsd.articles@gmail.com |
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1797052796247212032 |